Agromyces albus
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3965 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q2KWU5|A0A4Q2KWU5_9MICO Uncharacterized protein OS=Agromyces albus OX=205332 GN=ESP51_13830 PE=4 SV=1
MM1 pKa = 7.06 VSRR4 pKa = 11.84 RR5 pKa = 11.84 QRR7 pKa = 11.84 TALLAAGALVAGLIAPVALAAPVYY31 pKa = 10.51 AAVTCDD37 pKa = 3.09 VGGFEE42 pKa = 5.02 IDD44 pKa = 3.72 GNFTAEE50 pKa = 3.88 DD51 pKa = 4.12 CAPGFDD57 pKa = 4.25 DD58 pKa = 4.49 WDD60 pKa = 3.64 NVAYY64 pKa = 10.42 DD65 pKa = 3.59 STTQGGTYY73 pKa = 9.86 SASSKK78 pKa = 10.18 EE79 pKa = 4.26 DD80 pKa = 3.49 EE81 pKa = 5.57 DD82 pKa = 4.15 PAGWTSAGNTPDD94 pKa = 3.2 KK95 pKa = 10.9 GTFAEE100 pKa = 4.44 VFSFARR106 pKa = 11.84 IADD109 pKa = 3.69 GDD111 pKa = 4.25 YY112 pKa = 8.83 FTYY115 pKa = 10.71 VAWTRR120 pKa = 11.84 DD121 pKa = 3.54 NNTGTGGYY129 pKa = 10.21 AIEE132 pKa = 4.09 ITNAGEE138 pKa = 4.06 RR139 pKa = 11.84 VAEE142 pKa = 4.96 DD143 pKa = 3.68 GTPQPDD149 pKa = 3.27 RR150 pKa = 11.84 SLGGSVFYY158 pKa = 11.55 VEE160 pKa = 4.73 MQGSDD165 pKa = 3.47 LPDD168 pKa = 3.86 FLEE171 pKa = 4.96 GCVYY175 pKa = 10.62 TSVDD179 pKa = 4.31 DD180 pKa = 4.3 FPGVCSSDD188 pKa = 3.16 EE189 pKa = 3.86 TGYY192 pKa = 10.35 FAEE195 pKa = 4.49 ISEE198 pKa = 4.93 DD199 pKa = 3.54 GLFFEE204 pKa = 5.55 VGLNLTEE211 pKa = 4.29 LSGVAPGCPPVSEE224 pKa = 4.31 TTTVYY229 pKa = 10.41 VRR231 pKa = 11.84 SQTGQAGTEE240 pKa = 4.08 ANLKK244 pKa = 10.59 GYY246 pKa = 8.13 VAPLQVDD253 pKa = 4.0 PPSTCGEE260 pKa = 3.87 ILVDD264 pKa = 5.38 KK265 pKa = 8.38 EE266 pKa = 4.56 TLPDD270 pKa = 3.71 EE271 pKa = 4.33 YY272 pKa = 11.47 DD273 pKa = 3.28 KK274 pKa = 11.67 DD275 pKa = 4.09 FEE277 pKa = 4.24 FAFAGEE283 pKa = 4.37 EE284 pKa = 4.22 GTIDD288 pKa = 3.63 FTLNDD293 pKa = 3.57 ATDD296 pKa = 3.94 DD297 pKa = 4.14 EE298 pKa = 4.87 GALWSSGPIPAGTYY312 pKa = 7.47 TLEE315 pKa = 4.17 EE316 pKa = 4.38 LVPAGWTLDD325 pKa = 4.44 DD326 pKa = 4.17 ISCGTVEE333 pKa = 4.19 GHH335 pKa = 5.05 TTTIEE340 pKa = 3.98 IAEE343 pKa = 4.44 HH344 pKa = 5.8 EE345 pKa = 4.59 VVSCVFTNSLDD356 pKa = 3.85 VVPTEE361 pKa = 3.69 PAKK364 pKa = 10.37 PIKK367 pKa = 8.87 PTLAATGVDD376 pKa = 3.75 PMSGLWLGLLLFGAGGLVFTVRR398 pKa = 11.84 RR399 pKa = 11.84 ARR401 pKa = 11.84 ATRR404 pKa = 11.84 AA405 pKa = 3.08
Molecular weight: 42.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.567
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.681
Sillero 3.897
Patrickios 1.303
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A4Q2L775|A0A4Q2L775_9MICO Phosphatase PAP2 family protein OS=Agromyces albus OX=205332 GN=ESP51_05305 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3965
0
3965
1288838
29
2330
325.1
34.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.563 ± 0.059
0.474 ± 0.009
6.155 ± 0.036
6.059 ± 0.036
3.289 ± 0.023
9.008 ± 0.035
2.004 ± 0.018
4.568 ± 0.027
1.761 ± 0.028
10.042 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.73 ± 0.013
1.993 ± 0.024
5.453 ± 0.029
2.563 ± 0.017
7.324 ± 0.044
5.702 ± 0.028
5.859 ± 0.035
8.888 ± 0.037
1.555 ± 0.016
2.009 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here