Amino acid dipepetide frequency for Cryobacterium sp. TmT2-59

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.434AlaAla: 20.434 ± 0.222	0.703AlaCys: 0.703 ± 0.029	7.613AlaAsp: 7.613 ± 0.09	7.778AlaGlu: 7.778 ± 0.109	3.895AlaPhe: 3.895 ± 0.06	12.804AlaGly: 12.804 ± 0.133	2.324AlaHis: 2.324 ± 0.06	5.529AlaIle: 5.529 ± 0.074	2.796AlaLys: 2.796 ± 0.058	13.996AlaLeu: 13.996 ± 0.134	2.439AlaMet: 2.439 ± 0.044	2.598AlaAsn: 2.598 ± 0.056	6.205AlaPro: 6.205 ± 0.104	3.761AlaGln: 3.761 ± 0.076	8.83AlaArg: 8.83 ± 0.11	7.414AlaSer: 7.414 ± 0.108	7.626AlaThr: 7.626 ± 0.095	11.068AlaVal: 11.068 ± 0.112	1.788AlaTrp: 1.788 ± 0.043	2.208AlaTyr: 2.208 ± 0.047	0.002AlaXaa: 0.002 ± 0.001
Cys	0.671CysAla: 0.671 ± 0.029	0.058CysCys: 0.058 ± 0.008	0.335CysAsp: 0.335 ± 0.018	0.256CysGlu: 0.256 ± 0.017	0.183CysPhe: 0.183 ± 0.015	0.604CysGly: 0.604 ± 0.027	0.138CysHis: 0.138 ± 0.013	0.202CysIle: 0.202 ± 0.012	0.089CysLys: 0.089 ± 0.009	0.493CysLeu: 0.493 ± 0.023	0.08CysMet: 0.08 ± 0.008	0.139CysAsn: 0.139 ± 0.014	0.322CysPro: 0.322 ± 0.02	0.148CysGln: 0.148 ± 0.012	0.393CysArg: 0.393 ± 0.023	0.371CysSer: 0.371 ± 0.021	0.376CysThr: 0.376 ± 0.021	0.469CysVal: 0.469 ± 0.026	0.087CysTrp: 0.087 ± 0.009	0.11CysTyr: 0.11 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.961AspAla: 7.961 ± 0.084	0.281AspCys: 0.281 ± 0.018	3.326AspAsp: 3.326 ± 0.063	3.661AspGlu: 3.661 ± 0.061	1.878AspPhe: 1.878 ± 0.048	5.176AspGly: 5.176 ± 0.08	1.154AspHis: 1.154 ± 0.034	2.353AspIle: 2.353 ± 0.053	1.159AspLys: 1.159 ± 0.031	6.349AspLeu: 6.349 ± 0.089	0.786AspMet: 0.786 ± 0.029	1.128AspAsn: 1.128 ± 0.031	3.806AspPro: 3.806 ± 0.057	1.768AspGln: 1.768 ± 0.041	4.224AspArg: 4.224 ± 0.079	2.998AspSer: 2.998 ± 0.053	3.041AspThr: 3.041 ± 0.057	4.799AspVal: 4.799 ± 0.069	0.907AspTrp: 0.907 ± 0.032	1.28AspTyr: 1.28 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	6.401GluAla: 6.401 ± 0.09	0.246GluCys: 0.246 ± 0.016	2.352GluAsp: 2.352 ± 0.056	2.614GluGlu: 2.614 ± 0.06	1.752GluPhe: 1.752 ± 0.045	3.4GluGly: 3.4 ± 0.065	1.327GluHis: 1.327 ± 0.035	2.732GluIle: 2.732 ± 0.05	1.494GluLys: 1.494 ± 0.044	6.377GluLeu: 6.377 ± 0.099	0.983GluMet: 0.983 ± 0.034	1.288GluAsn: 1.288 ± 0.033	2.788GluPro: 2.788 ± 0.059	2.111GluGln: 2.111 ± 0.045	4.391GluArg: 4.391 ± 0.086	2.973GluSer: 2.973 ± 0.051	3.12GluThr: 3.12 ± 0.052	4.176GluVal: 4.176 ± 0.076	0.768GluTrp: 0.768 ± 0.03	1.05GluTyr: 1.05 ± 0.034	0.001GluXaa: 0.001 ± 0.001
Phe	4.145PheAla: 4.145 ± 0.07	0.191PheCys: 0.191 ± 0.014	2.295PheAsp: 2.295 ± 0.047	1.741PheGlu: 1.741 ± 0.045	1.133PhePhe: 1.133 ± 0.038	3.541PheGly: 3.541 ± 0.065	0.6PheHis: 0.6 ± 0.022	1.324PheIle: 1.324 ± 0.042	0.621PheLys: 0.621 ± 0.025	3.101PheLeu: 3.101 ± 0.065	0.504PheMet: 0.504 ± 0.023	0.767PheAsn: 0.767 ± 0.027	1.481PhePro: 1.481 ± 0.037	0.817PheGln: 0.817 ± 0.03	1.771PheArg: 1.771 ± 0.039	1.966PheSer: 1.966 ± 0.046	2.216PheThr: 2.216 ± 0.053	2.879PheVal: 2.879 ± 0.052	0.466PheTrp: 0.466 ± 0.021	0.718PheTyr: 0.718 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	10.478GlyAla: 10.478 ± 0.126	0.605GlyCys: 0.605 ± 0.029	4.588GlyAsp: 4.588 ± 0.071	4.359GlyGlu: 4.359 ± 0.064	3.312GlyPhe: 3.312 ± 0.06	7.275GlyGly: 7.275 ± 0.11	1.915GlyHis: 1.915 ± 0.053	4.854GlyIle: 4.854 ± 0.071	2.395GlyLys: 2.395 ± 0.054	9.566GlyLeu: 9.566 ± 0.104	1.903GlyMet: 1.903 ± 0.043	1.888GlyAsn: 1.888 ± 0.049	4.021GlyPro: 4.021 ± 0.064	2.831GlyGln: 2.831 ± 0.058	6.262GlyArg: 6.262 ± 0.076	5.774GlySer: 5.774 ± 0.086	5.927GlyThr: 5.927 ± 0.078	7.554GlyVal: 7.554 ± 0.093	1.541GlyTrp: 1.541 ± 0.042	2.231GlyTyr: 2.231 ± 0.048	0.001GlyXaa: 0.001 ± 0.001
His	2.235HisAla: 2.235 ± 0.054	0.146HisCys: 0.146 ± 0.012	1.243HisAsp: 1.243 ± 0.036	1.087HisGlu: 1.087 ± 0.033	0.546HisPhe: 0.546 ± 0.02	1.94HisGly: 1.94 ± 0.046	0.534HisHis: 0.534 ± 0.023	0.737HisIle: 0.737 ± 0.028	0.326HisLys: 0.326 ± 0.017	2.081HisLeu: 2.081 ± 0.046	0.308HisMet: 0.308 ± 0.018	0.413HisAsn: 0.413 ± 0.023	1.534HisPro: 1.534 ± 0.041	0.582HisGln: 0.582 ± 0.021	1.552HisArg: 1.552 ± 0.044	1.08HisSer: 1.08 ± 0.033	1.117HisThr: 1.117 ± 0.035	1.506HisVal: 1.506 ± 0.043	0.266HisTrp: 0.266 ± 0.017	0.425HisTyr: 0.425 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.306IleAla: 6.306 ± 0.08	0.285IleCys: 0.285 ± 0.02	3.434IleAsp: 3.434 ± 0.064	2.727IleGlu: 2.727 ± 0.052	1.31IlePhe: 1.31 ± 0.037	4.597IleGly: 4.597 ± 0.084	0.722IleHis: 0.722 ± 0.025	1.989IleIle: 1.989 ± 0.053	0.97IleLys: 0.97 ± 0.034	4.161IleLeu: 4.161 ± 0.068	0.705IleMet: 0.705 ± 0.027	1.15IleAsn: 1.15 ± 0.036	2.403IlePro: 2.403 ± 0.05	1.058IleGln: 1.058 ± 0.032	3.019IleArg: 3.019 ± 0.054	2.592IleSer: 2.592 ± 0.053	2.89IleThr: 2.89 ± 0.05	4.563IleVal: 4.563 ± 0.077	0.494IleTrp: 0.494 ± 0.028	0.813IleTyr: 0.813 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	2.742LysAla: 2.742 ± 0.06	0.096LysCys: 0.096 ± 0.009	1.169LysAsp: 1.169 ± 0.038	1.005LysGlu: 1.005 ± 0.038	0.649LysPhe: 0.649 ± 0.027	1.644LysGly: 1.644 ± 0.044	0.457LysHis: 0.457 ± 0.022	1.138LysIle: 1.138 ± 0.039	0.913LysLys: 0.913 ± 0.042	2.17LysLeu: 2.17 ± 0.049	0.481LysMet: 0.481 ± 0.022	0.65LysAsn: 0.65 ± 0.024	1.365LysPro: 1.365 ± 0.033	0.783LysGln: 0.783 ± 0.029	1.698LysArg: 1.698 ± 0.036	1.465LysSer: 1.465 ± 0.039	1.586LysThr: 1.586 ± 0.039	1.86LysVal: 1.86 ± 0.046	0.281LysTrp: 0.281 ± 0.016	0.483LysTyr: 0.483 ± 0.022	0.001LysXaa: 0.001 ± 0.001
Leu	15.356LeuAla: 15.356 ± 0.136	0.6LeuCys: 0.6 ± 0.023	6.391LeuAsp: 6.391 ± 0.092	5.009LeuGlu: 5.009 ± 0.079	3.026LeuPhe: 3.026 ± 0.06	9.793LeuGly: 9.793 ± 0.105	1.966LeuHis: 1.966 ± 0.042	4.929LeuIle: 4.929 ± 0.074	2.165LeuLys: 2.165 ± 0.053	11.202LeuLeu: 11.202 ± 0.144	1.802LeuMet: 1.802 ± 0.048	2.29LeuAsn: 2.29 ± 0.045	5.646LeuPro: 5.646 ± 0.083	2.604LeuGln: 2.604 ± 0.051	7.35LeuArg: 7.35 ± 0.107	6.31LeuSer: 6.31 ± 0.089	6.933LeuThr: 6.933 ± 0.078	9.485LeuVal: 9.485 ± 0.114	1.215LeuTrp: 1.215 ± 0.038	1.678LeuTyr: 1.678 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.233MetAla: 2.233 ± 0.044	0.101MetCys: 0.101 ± 0.009	0.827MetAsp: 0.827 ± 0.031	0.669MetGlu: 0.669 ± 0.026	0.548MetPhe: 0.548 ± 0.023	1.32MetGly: 1.32 ± 0.037	0.332MetHis: 0.332 ± 0.019	0.951MetIle: 0.951 ± 0.028	0.56MetLys: 0.56 ± 0.023	1.95MetLeu: 1.95 ± 0.039	0.334MetMet: 0.334 ± 0.017	0.535MetAsn: 0.535 ± 0.024	1.07MetPro: 1.07 ± 0.033	0.535MetGln: 0.535 ± 0.021	1.28MetArg: 1.28 ± 0.036	1.446MetSer: 1.446 ± 0.037	1.627MetThr: 1.627 ± 0.038	1.44MetVal: 1.44 ± 0.041	0.199MetTrp: 0.199 ± 0.013	0.264MetTyr: 0.264 ± 0.015	0.001MetXaa: 0.001 ± 0.001
Asn	2.685AsnAla: 2.685 ± 0.054	0.121AsnCys: 0.121 ± 0.011	1.273AsnAsp: 1.273 ± 0.039	1.1AsnGlu: 1.1 ± 0.038	0.758AsnPhe: 0.758 ± 0.028	2.138AsnGly: 2.138 ± 0.049	0.389AsnHis: 0.389 ± 0.021	0.942AsnIle: 0.942 ± 0.031	0.498AsnLys: 0.498 ± 0.025	2.381AsnLeu: 2.381 ± 0.046	0.367AsnMet: 0.367 ± 0.019	0.585AsnAsn: 0.585 ± 0.026	1.706AsnPro: 1.706 ± 0.041	0.717AsnGln: 0.717 ± 0.026	1.605AsnArg: 1.605 ± 0.042	1.172AsnSer: 1.172 ± 0.036	1.352AsnThr: 1.352 ± 0.038	1.853AsnVal: 1.853 ± 0.048	0.344AsnTrp: 0.344 ± 0.019	0.541AsnTyr: 0.541 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	7.645ProAla: 7.645 ± 0.1	0.207ProCys: 0.207 ± 0.014	3.665ProAsp: 3.665 ± 0.07	3.427ProGlu: 3.427 ± 0.056	1.703ProPhe: 1.703 ± 0.043	5.371ProGly: 5.371 ± 0.082	1.042ProHis: 1.042 ± 0.033	2.1ProIle: 2.1 ± 0.042	1.17ProLys: 1.17 ± 0.037	4.898ProLeu: 4.898 ± 0.069	0.945ProMet: 0.945 ± 0.033	1.201ProAsn: 1.201 ± 0.039	2.247ProPro: 2.247 ± 0.062	1.471ProGln: 1.471 ± 0.039	3.269ProArg: 3.269 ± 0.065	3.081ProSer: 3.081 ± 0.053	3.361ProThr: 3.361 ± 0.078	5.025ProVal: 5.025 ± 0.071	0.768ProTrp: 0.768 ± 0.032	0.986ProTyr: 0.986 ± 0.03	0.001ProXaa: 0.001 ± 0.001
Gln	3.99GlnAla: 3.99 ± 0.066	0.136GlnCys: 0.136 ± 0.014	1.353GlnAsp: 1.353 ± 0.037	1.25GlnGlu: 1.25 ± 0.034	0.91GlnPhe: 0.91 ± 0.028	2.17GlnGly: 2.17 ± 0.051	0.621GlnHis: 0.621 ± 0.026	1.523GlnIle: 1.523 ± 0.036	0.771GlnLys: 0.771 ± 0.025	3.313GlnLeu: 3.313 ± 0.06	0.551GlnMet: 0.551 ± 0.022	0.737GlnAsn: 0.737 ± 0.03	1.528GlnPro: 1.528 ± 0.039	1.074GlnGln: 1.074 ± 0.033	2.214GlnArg: 2.214 ± 0.053	1.726GlnSer: 1.726 ± 0.042	1.69GlnThr: 1.69 ± 0.044	2.63GlnVal: 2.63 ± 0.051	0.413GlnTrp: 0.413 ± 0.021	0.562GlnTyr: 0.562 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	8.278ArgAla: 8.278 ± 0.097	0.358ArgCys: 0.358 ± 0.021	3.92ArgAsp: 3.92 ± 0.068	3.799ArgGlu: 3.799 ± 0.074	2.406ArgPhe: 2.406 ± 0.051	5.187ArgGly: 5.187 ± 0.083	1.559ArgHis: 1.559 ± 0.04	3.436ArgIle: 3.436 ± 0.062	1.564ArgLys: 1.564 ± 0.042	7.619ArgLeu: 7.619 ± 0.111	1.702ArgMet: 1.702 ± 0.045	1.551ArgAsn: 1.551 ± 0.04	3.69ArgPro: 3.69 ± 0.087	2.233ArgGln: 2.233 ± 0.05	5.981ArgArg: 5.981 ± 0.099	4.347ArgSer: 4.347 ± 0.069	4.195ArgThr: 4.195 ± 0.072	5.602ArgVal: 5.602 ± 0.071	1.11ArgTrp: 1.11 ± 0.035	1.533ArgTyr: 1.533 ± 0.044	0.002ArgXaa: 0.002 ± 0.001
Ser	7.285SerAla: 7.285 ± 0.103	0.312SerCys: 0.312 ± 0.018	3.113SerAsp: 3.113 ± 0.065	2.798SerGlu: 2.798 ± 0.061	2.039SerPhe: 2.039 ± 0.043	6.154SerGly: 6.154 ± 0.081	1.056SerHis: 1.056 ± 0.03	2.768SerIle: 2.768 ± 0.064	1.263SerLys: 1.263 ± 0.038	5.948SerLeu: 5.948 ± 0.085	1.243SerMet: 1.243 ± 0.033	1.252SerAsn: 1.252 ± 0.037	3.329SerPro: 3.329 ± 0.062	1.55SerGln: 1.55 ± 0.04	3.986SerArg: 3.986 ± 0.062	3.688SerSer: 3.688 ± 0.064	3.912SerThr: 3.912 ± 0.07	5.504SerVal: 5.504 ± 0.075	0.863SerTrp: 0.863 ± 0.029	1.327SerTyr: 1.327 ± 0.04	0.001SerXaa: 0.001 ± 0.001
Thr	8.067ThrAla: 8.067 ± 0.106	0.313ThrCys: 0.313 ± 0.021	4.007ThrAsp: 4.007 ± 0.062	3.249ThrGlu: 3.249 ± 0.065	1.919ThrPhe: 1.919 ± 0.054	6.355ThrGly: 6.355 ± 0.095	1.166ThrHis: 1.166 ± 0.036	2.945ThrIle: 2.945 ± 0.06	1.305ThrLys: 1.305 ± 0.04	6.274ThrLeu: 6.274 ± 0.086	1.063ThrMet: 1.063 ± 0.028	1.384ThrAsn: 1.384 ± 0.038	3.9ThrPro: 3.9 ± 0.066	1.542ThrGln: 1.542 ± 0.036	3.965ThrArg: 3.965 ± 0.069	3.537ThrSer: 3.537 ± 0.07	3.799ThrThr: 3.799 ± 0.073	6.032ThrVal: 6.032 ± 0.085	0.797ThrTrp: 0.797 ± 0.03	1.139ThrTyr: 1.139 ± 0.036	0.001ThrXaa: 0.001 ± 0.001
Val	11.097ValAla: 11.097 ± 0.11	0.534ValCys: 0.534 ± 0.027	5.218ValAsp: 5.218 ± 0.066	4.284ValGlu: 4.284 ± 0.074	3.029ValPhe: 3.029 ± 0.053	7.04ValGly: 7.04 ± 0.086	1.732ValHis: 1.732 ± 0.04	4.399ValIle: 4.399 ± 0.069	1.835ValLys: 1.835 ± 0.048	9.591ValLeu: 9.591 ± 0.103	1.457ValMet: 1.457 ± 0.042	2.055ValAsn: 2.055 ± 0.043	4.737ValPro: 4.737 ± 0.075	2.371ValGln: 2.371 ± 0.046	5.585ValArg: 5.585 ± 0.085	5.393ValSer: 5.393 ± 0.079	6.017ValThr: 6.017 ± 0.084	8.11ValVal: 8.11 ± 0.105	1.057ValTrp: 1.057 ± 0.037	1.568ValTyr: 1.568 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.552TrpAla: 1.552 ± 0.035	0.096TrpCys: 0.096 ± 0.009	0.706TrpAsp: 0.706 ± 0.022	0.524TrpGlu: 0.524 ± 0.023	0.537TrpPhe: 0.537 ± 0.021	0.939TrpGly: 0.939 ± 0.037	0.308TrpHis: 0.308 ± 0.017	0.658TrpIle: 0.658 ± 0.024	0.333TrpLys: 0.333 ± 0.019	1.864TrpLeu: 1.864 ± 0.048	0.329TrpMet: 0.329 ± 0.019	0.406TrpAsn: 0.406 ± 0.023	0.706TrpPro: 0.706 ± 0.027	0.591TrpGln: 0.591 ± 0.023	1.072TrpArg: 1.072 ± 0.033	0.853TrpSer: 0.853 ± 0.032	0.857TrpThr: 0.857 ± 0.029	1.053TrpVal: 1.053 ± 0.034	0.313TrpTrp: 0.313 ± 0.019	0.286TrpTyr: 0.286 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.25TyrAla: 2.25 ± 0.047	0.135TyrCys: 0.135 ± 0.01	1.205TyrAsp: 1.205 ± 0.04	1.006TyrGlu: 1.006 ± 0.032	0.743TyrPhe: 0.743 ± 0.024	1.83TyrGly: 1.83 ± 0.043	0.316TyrHis: 0.316 ± 0.02	0.681TyrIle: 0.681 ± 0.026	0.407TyrLys: 0.407 ± 0.018	2.414TyrLeu: 2.414 ± 0.054	0.239TyrMet: 0.239 ± 0.015	0.512TyrAsn: 0.512 ± 0.021	1.098TyrPro: 1.098 ± 0.04	0.614TyrGln: 0.614 ± 0.025	1.621TyrArg: 1.621 ± 0.04	1.202TyrSer: 1.202 ± 0.031	1.119TyrThr: 1.119 ± 0.032	1.503TyrVal: 1.503 ± 0.038	0.308TyrTrp: 0.308 ± 0.017	0.472TyrTyr: 0.472 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.003XaaGly: 0.003 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3217 proteins (1037322 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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