Deerpox virus (strain Mule deer/United States/W-848-83/1983) (DPV)
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 165 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q08FY7|Q08FY7_DPV83 CC chemokine receptor-like protein OS=Deerpox virus (strain Mule deer/United States/W-848-83/1983) OX=305674 GN=DpV83gp013 PE=4 SV=1
MM1 pKa = 8.22 DD2 pKa = 4.41 MMSKK6 pKa = 10.16 EE7 pKa = 4.74 CDD9 pKa = 3.34 FMDD12 pKa = 3.36 QVDD15 pKa = 4.06 YY16 pKa = 10.84 MFSKK20 pKa = 10.77 YY21 pKa = 10.6 SNPLFYY27 pKa = 10.87 YY28 pKa = 9.99 IEE30 pKa = 4.94 SDD32 pKa = 4.14 DD33 pKa = 3.97 EE34 pKa = 4.52 EE35 pKa = 5.02 GVKK38 pKa = 10.17 KK39 pKa = 9.98 WINLSNEE46 pKa = 3.93 NNDD49 pKa = 3.37 FNEE52 pKa = 4.19 PPILSCLDD60 pKa = 3.22 KK61 pKa = 11.08 EE62 pKa = 4.56 YY63 pKa = 11.41 VNKK66 pKa = 10.62 NILKK70 pKa = 10.41 LLIEE74 pKa = 4.48 NGANVNCIIPGDD86 pKa = 4.2 NISLLSYY93 pKa = 10.53 YY94 pKa = 9.5 LTYY97 pKa = 10.93 NKK99 pKa = 10.2 NISEE103 pKa = 4.95 EE104 pKa = 3.83 ILKK107 pKa = 10.31 ILIEE111 pKa = 4.33 ANANINEE118 pKa = 4.13 INSDD122 pKa = 3.84 GKK124 pKa = 10.54 NLLHH128 pKa = 6.97 VYY130 pKa = 10.33 LDD132 pKa = 4.01 NISINVEE139 pKa = 3.39 IMKK142 pKa = 9.91 FLIEE146 pKa = 4.27 MGNSPFNKK154 pKa = 9.72 DD155 pKa = 2.66 KK156 pKa = 10.86 HH157 pKa = 6.31 QNNILYY163 pKa = 8.73 TYY165 pKa = 11.22 LMYY168 pKa = 10.58 HH169 pKa = 7.58 DD170 pKa = 4.94 DD171 pKa = 3.66 QKK173 pKa = 11.36 IVDD176 pKa = 4.27 FLLSIGLDD184 pKa = 3.49 YY185 pKa = 11.25 EE186 pKa = 4.34 EE187 pKa = 5.16 CNNMGLNCIDD197 pKa = 3.03 ISKK200 pKa = 10.19 NRR202 pKa = 11.84 DD203 pKa = 2.87 IIFNKK208 pKa = 10.28 DD209 pKa = 2.59
Molecular weight: 24.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.003
IPC2_protein 4.291
IPC_protein 4.253
Toseland 4.062
ProMoST 4.342
Dawson 4.215
Bjellqvist 4.393
Wikipedia 4.113
Rodwell 4.075
Grimsley 3.973
Solomon 4.215
Lehninger 4.164
Nozaki 4.329
DTASelect 4.52
Thurlkill 4.088
EMBOSS 4.126
Sillero 4.368
Patrickios 1.977
IPC_peptide 4.215
IPC2_peptide 4.355
IPC2.peptide.svr19 4.269
Protein with the highest isoelectric point:
>tr|Q08FV8|Q08FV8_DPV83 DNA-directed RNA polymerase 30 kDa polypeptide OS=Deerpox virus (strain Mule deer/United States/W-848-83/1983) OX=305674 GN=DpV83gp043 PE=3 SV=1
MM1 pKa = 7.51 ALSAKK6 pKa = 9.8 EE7 pKa = 3.78 IFSAISLTLLALLMIISGGALAFKK31 pKa = 10.54 SLAPHH36 pKa = 7.17 RR37 pKa = 11.84 IVSMRR42 pKa = 11.84 SATFNRR48 pKa = 11.84 VISILEE54 pKa = 3.78 YY55 pKa = 10.45 LAILIFIPGTIALYY69 pKa = 10.05 SAYY72 pKa = 9.43 IKK74 pKa = 10.8 KK75 pKa = 10.35 LFSS78 pKa = 3.42
Molecular weight: 8.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.218
IPC2_protein 9.794
IPC_protein 10.058
Toseland 10.248
ProMoST 9.94
Dawson 10.438
Bjellqvist 10.116
Wikipedia 10.628
Rodwell 10.891
Grimsley 10.526
Solomon 10.482
Lehninger 10.452
Nozaki 10.204
DTASelect 10.116
Thurlkill 10.292
EMBOSS 10.643
Sillero 10.35
Patrickios 10.716
IPC_peptide 10.482
IPC2_peptide 8.785
IPC2.peptide.svr19 8.694
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
159
6
165
51100
53
1953
309.7
35.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.771 ± 0.119
2.008 ± 0.085
6.162 ± 0.112
5.611 ± 0.145
5.29 ± 0.136
3.595 ± 0.117
1.722 ± 0.06
11.614 ± 0.22
9.088 ± 0.159
9.112 ± 0.149
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.493 ± 0.073
8.384 ± 0.172
2.947 ± 0.1
2.022 ± 0.07
3.184 ± 0.101
7.706 ± 0.151
5.341 ± 0.141
4.806 ± 0.123
0.654 ± 0.04
5.491 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here