Solibacillus sp. R5-41

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Solibacillus; unclassified Solibacillus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3944 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1SR98|A0A2D1SR98_9BACL DUF2179 domain-containing protein OS=Solibacillus sp. R5-41 OX=2048654 GN=CSE16_05995 PE=4 SV=1
MM1 pKa = 7.1VFSFFKK7 pKa = 10.39KK8 pKa = 9.92KK9 pKa = 10.6KK10 pKa = 9.67EE11 pKa = 3.85GTVLKK16 pKa = 10.48NQVEE20 pKa = 4.66FCMTNLSLGAADD32 pKa = 4.33VYY34 pKa = 11.45DD35 pKa = 4.04VLIEE39 pKa = 4.3LDD41 pKa = 3.64DD42 pKa = 4.23VEE44 pKa = 4.52ITEE47 pKa = 4.69SGCTSNCEE55 pKa = 3.48ICEE58 pKa = 4.14CHH60 pKa = 7.14LFAIVNGEE68 pKa = 4.03MVLGDD73 pKa = 4.56DD74 pKa = 5.87ADD76 pKa = 4.04TLLQNVQQEE85 pKa = 4.6LEE87 pKa = 4.44VNSVV91 pKa = 2.79

Molecular weight: 10.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1SZ96|A0A2D1SZ96_9BACL Uncharacterized protein OS=Solibacillus sp. R5-41 OX=2048654 GN=CSE16_06460 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.08NGRR28 pKa = 11.84NVLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3944

0

3944

1115748

30

1777

282.9

31.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.248 ± 0.049

0.717 ± 0.013

4.911 ± 0.029

7.364 ± 0.043

4.791 ± 0.035

6.486 ± 0.046

2.022 ± 0.02

8.28 ± 0.037

6.67 ± 0.036

9.735 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.824 ± 0.021

4.673 ± 0.033

3.388 ± 0.026

4.076 ± 0.028

3.72 ± 0.026

5.792 ± 0.031

5.697 ± 0.031

7.038 ± 0.035

0.976 ± 0.016

3.592 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski