Amino acid dipepetide frequency for Epsilonproteobacteria bacterium SCGC AD-308-I21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.017AlaAla: 4.017 ± 0.202
0.665AlaCys: 0.665 ± 0.066
3.41AlaAsp: 3.41 ± 0.134
3.453AlaGlu: 3.453 ± 0.128
3.199AlaPhe: 3.199 ± 0.117
4.065AlaGly: 4.065 ± 0.189
1.363AlaHis: 1.363 ± 0.087
5.643AlaIle: 5.643 ± 0.185
5.891AlaLys: 5.891 ± 0.189
7.441AlaLeu: 7.441 ± 0.219
1.961AlaMet: 1.961 ± 0.1
3.161AlaAsn: 3.161 ± 0.121
1.788AlaPro: 1.788 ± 0.096
2.248AlaGln: 2.248 ± 0.097
2.401AlaArg: 2.401 ± 0.13
4.156AlaSer: 4.156 ± 0.176
3.653AlaThr: 3.653 ± 0.159
3.993AlaVal: 3.993 ± 0.159
0.512AlaTrp: 0.512 ± 0.064
2.764AlaTyr: 2.764 ± 0.114
0.005AlaXaa: 0.005 ± 0.005
Cys
0.464CysAla: 0.464 ± 0.046
0.077CysCys: 0.077 ± 0.02
0.56CysAsp: 0.56 ± 0.053
0.713CysGlu: 0.713 ± 0.062
0.306CysPhe: 0.306 ± 0.041
0.55CysGly: 0.55 ± 0.059
0.253CysHis: 0.253 ± 0.042
0.488CysIle: 0.488 ± 0.055
0.622CysLys: 0.622 ± 0.051
0.545CysLeu: 0.545 ± 0.051
0.201CysMet: 0.201 ± 0.03
0.378CysAsn: 0.378 ± 0.055
0.306CysPro: 0.306 ± 0.046
0.206CysGln: 0.206 ± 0.033
0.253CysArg: 0.253 ± 0.036
0.679CysSer: 0.679 ± 0.061
0.43CysThr: 0.43 ± 0.048
0.397CysVal: 0.397 ± 0.051
0.072CysTrp: 0.072 ± 0.018
0.282CysTyr: 0.282 ± 0.041
0.0CysXaa: 0.0 ± 0.0
Asp
3.979AspAla: 3.979 ± 0.154
0.435AspCys: 0.435 ± 0.047
3.386AspAsp: 3.386 ± 0.135
5.198AspGlu: 5.198 ± 0.174
3.247AspPhe: 3.247 ± 0.132
3.524AspGly: 3.524 ± 0.149
0.65AspHis: 0.65 ± 0.06
6.045AspIle: 6.045 ± 0.191
4.988AspLys: 4.988 ± 0.193
5.064AspLeu: 5.064 ± 0.158
1.855AspMet: 1.855 ± 0.089
2.917AspAsn: 2.917 ± 0.145
1.253AspPro: 1.253 ± 0.08
0.961AspGln: 0.961 ± 0.066
1.645AspArg: 1.645 ± 0.082
3.558AspSer: 3.558 ± 0.141
3.094AspThr: 3.094 ± 0.122
3.577AspVal: 3.577 ± 0.135
0.43AspTrp: 0.43 ± 0.043
2.53AspTyr: 2.53 ± 0.128
0.0AspXaa: 0.0 ± 0.0
Glu
4.801GluAla: 4.801 ± 0.158
0.569GluCys: 0.569 ± 0.049
3.998GluAsp: 3.998 ± 0.135
5.208GluGlu: 5.208 ± 0.189
3.61GluPhe: 3.61 ± 0.158
3.324GluGly: 3.324 ± 0.133
1.688GluHis: 1.688 ± 0.109
6.628GluIle: 6.628 ± 0.199
6.566GluLys: 6.566 ± 0.204
7.929GluLeu: 7.929 ± 0.217
2.023GluMet: 2.023 ± 0.114
4.605GluAsn: 4.605 ± 0.156
1.535GluPro: 1.535 ± 0.094
2.271GluGln: 2.271 ± 0.099
2.123GluArg: 2.123 ± 0.114
4.055GluSer: 4.055 ± 0.141
2.821GluThr: 2.821 ± 0.12
4.519GluVal: 4.519 ± 0.158
0.521GluTrp: 0.521 ± 0.044
3.056GluTyr: 3.056 ± 0.129
0.0GluXaa: 0.0 ± 0.0
Phe
3.147PheAla: 3.147 ± 0.128
0.392PheCys: 0.392 ± 0.046
3.084PheAsp: 3.084 ± 0.118
3.371PheGlu: 3.371 ± 0.13
2.597PhePhe: 2.597 ± 0.126
3.156PheGly: 3.156 ± 0.155
0.823PheHis: 0.823 ± 0.057
4.18PheIle: 4.18 ± 0.153
3.682PheLys: 3.682 ± 0.159
4.682PheLeu: 4.682 ± 0.17
1.387PheMet: 1.387 ± 0.095
2.453PheAsn: 2.453 ± 0.115
1.224PhePro: 1.224 ± 0.072
1.047PheGln: 1.047 ± 0.074
1.482PheArg: 1.482 ± 0.085
3.687PheSer: 3.687 ± 0.14
2.75PheThr: 2.75 ± 0.121
3.08PheVal: 3.08 ± 0.117
0.426PheTrp: 0.426 ± 0.044
2.114PheTyr: 2.114 ± 0.107
0.0PheXaa: 0.0 ± 0.0
Gly
3.869GlyAla: 3.869 ± 0.179
0.674GlyCys: 0.674 ± 0.06
3.199GlyAsp: 3.199 ± 0.151
3.697GlyGlu: 3.697 ± 0.147
3.089GlyPhe: 3.089 ± 0.14
3.773GlyGly: 3.773 ± 0.168
1.124GlyHis: 1.124 ± 0.073
4.763GlyIle: 4.763 ± 0.145
4.208GlyLys: 4.208 ± 0.14
5.16GlyLeu: 5.16 ± 0.176
1.573GlyMet: 1.573 ± 0.084
2.195GlyAsn: 2.195 ± 0.126
0.846GlyPro: 0.846 ± 0.061
1.368GlyGln: 1.368 ± 0.097
1.989GlyArg: 1.989 ± 0.111
3.649GlySer: 3.649 ± 0.141
3.194GlyThr: 3.194 ± 0.122
4.548GlyVal: 4.548 ± 0.157
0.454GlyTrp: 0.454 ± 0.043
2.697GlyTyr: 2.697 ± 0.107
0.0GlyXaa: 0.0 ± 0.0
His
1.353HisAla: 1.353 ± 0.086
0.158HisCys: 0.158 ± 0.027
1.119HisAsp: 1.119 ± 0.071
1.32HisGlu: 1.32 ± 0.073
1.019HisPhe: 1.019 ± 0.065
1.277HisGly: 1.277 ± 0.083
0.631HisHis: 0.631 ± 0.053
1.597HisIle: 1.597 ± 0.095
1.516HisLys: 1.516 ± 0.094
1.717HisLeu: 1.717 ± 0.085
0.55HisMet: 0.55 ± 0.061
1.028HisAsn: 1.028 ± 0.067
0.803HisPro: 0.803 ± 0.065
0.693HisGln: 0.693 ± 0.056
0.736HisArg: 0.736 ± 0.06
1.291HisSer: 1.291 ± 0.097
1.148HisThr: 1.148 ± 0.064
1.401HisVal: 1.401 ± 0.083
0.177HisTrp: 0.177 ± 0.025
0.827HisTyr: 0.827 ± 0.059
0.0HisXaa: 0.0 ± 0.0
Ile
6.293IleAla: 6.293 ± 0.174
0.698IleCys: 0.698 ± 0.059
5.619IleAsp: 5.619 ± 0.173
6.623IleGlu: 6.623 ± 0.181
4.026IlePhe: 4.026 ± 0.153
4.782IleGly: 4.782 ± 0.16
1.535IleHis: 1.535 ± 0.075
6.896IleIle: 6.896 ± 0.254
7.039IleLys: 7.039 ± 0.239
8.326IleLeu: 8.326 ± 0.197
2.013IleMet: 2.013 ± 0.098
4.878IleAsn: 4.878 ± 0.178
2.491IlePro: 2.491 ± 0.107
2.496IleGln: 2.496 ± 0.123
2.606IleArg: 2.606 ± 0.111
6.465IleSer: 6.465 ± 0.169
4.175IleThr: 4.175 ± 0.134
5.318IleVal: 5.318 ± 0.185
0.454IleTrp: 0.454 ± 0.048
3.218IleTyr: 3.218 ± 0.15
0.005IleXaa: 0.005 ± 0.004
Lys
5.265LysAla: 5.265 ± 0.166
0.43LysCys: 0.43 ± 0.056
5.318LysAsp: 5.318 ± 0.164
7.752LysGlu: 7.752 ± 0.218
3.008LysPhe: 3.008 ± 0.138
3.429LysGly: 3.429 ± 0.13
1.736LysHis: 1.736 ± 0.099
7.374LysIle: 7.374 ± 0.248
7.871LysLys: 7.871 ± 0.254
7.685LysLeu: 7.685 ± 0.216
2.343LysMet: 2.343 ± 0.115
6.212LysAsn: 6.212 ± 0.193
2.501LysPro: 2.501 ± 0.121
2.807LysGln: 2.807 ± 0.111
2.735LysArg: 2.735 ± 0.109
5.442LysSer: 5.442 ± 0.178
4.18LysThr: 4.18 ± 0.143
4.71LysVal: 4.71 ± 0.153
0.607LysTrp: 0.607 ± 0.055
3.376LysTyr: 3.376 ± 0.149
0.0LysXaa: 0.0 ± 0.0
Leu
6.26LeuAla: 6.26 ± 0.193
0.674LeuCys: 0.674 ± 0.06
5.59LeuAsp: 5.59 ± 0.172
7.374LeuGlu: 7.374 ± 0.215
4.892LeuPhe: 4.892 ± 0.158
5.552LeuGly: 5.552 ± 0.205
2.19LeuHis: 2.19 ± 0.116
6.948LeuIle: 6.948 ± 0.205
8.856LeuLys: 8.856 ± 0.222
9.296LeuLeu: 9.296 ± 0.252
2.276LeuMet: 2.276 ± 0.102
5.624LeuAsn: 5.624 ± 0.21
3.075LeuPro: 3.075 ± 0.124
3.333LeuGln: 3.333 ± 0.135
3.089LeuArg: 3.089 ± 0.113
8.22LeuSer: 8.22 ± 0.216
4.816LeuThr: 4.816 ± 0.17
5.552LeuVal: 5.552 ± 0.154
0.603LeuTrp: 0.603 ± 0.058
3.697LeuTyr: 3.697 ± 0.121
0.0LeuXaa: 0.0 ± 0.0
Met
1.817MetAla: 1.817 ± 0.119
0.139MetCys: 0.139 ± 0.025
1.306MetAsp: 1.306 ± 0.079
1.42MetGlu: 1.42 ± 0.078
1.143MetPhe: 1.143 ± 0.08
1.616MetGly: 1.616 ± 0.092
0.55MetHis: 0.55 ± 0.05
2.219MetIle: 2.219 ± 0.096
2.338MetLys: 2.338 ± 0.115
2.707MetLeu: 2.707 ± 0.12
0.87MetMet: 0.87 ± 0.059
1.53MetAsn: 1.53 ± 0.102
0.995MetPro: 0.995 ± 0.071
1.172MetGln: 1.172 ± 0.082
1.033MetArg: 1.033 ± 0.069
2.271MetSer: 2.271 ± 0.112
1.353MetThr: 1.353 ± 0.077
1.506MetVal: 1.506 ± 0.086
0.215MetTrp: 0.215 ± 0.033
0.727MetTyr: 0.727 ± 0.054
0.0MetXaa: 0.0 ± 0.0
Asn
3.3AsnAla: 3.3 ± 0.13
0.373AsnCys: 0.373 ± 0.046
3.099AsnAsp: 3.099 ± 0.131
3.821AsnGlu: 3.821 ± 0.137
2.396AsnPhe: 2.396 ± 0.116
2.979AsnGly: 2.979 ± 0.125
0.971AsnHis: 0.971 ± 0.066
5.82AsnIle: 5.82 ± 0.181
4.543AsnLys: 4.543 ± 0.182
4.921AsnLeu: 4.921 ± 0.184
1.315AsnMet: 1.315 ± 0.079
3.171AsnAsn: 3.171 ± 0.144
2.028AsnPro: 2.028 ± 0.093
1.707AsnGln: 1.707 ± 0.083
1.855AsnArg: 1.855 ± 0.091
4.06AsnSer: 4.06 ± 0.146
2.927AsnThr: 2.927 ± 0.119
2.979AsnVal: 2.979 ± 0.105
0.306AsnTrp: 0.306 ± 0.041
2.147AsnTyr: 2.147 ± 0.11
0.0AsnXaa: 0.0 ± 0.0
Pro
1.583ProAla: 1.583 ± 0.092
0.191ProCys: 0.191 ± 0.032
1.583ProAsp: 1.583 ± 0.089
2.075ProGlu: 2.075 ± 0.108
1.588ProPhe: 1.588 ± 0.092
1.129ProGly: 1.129 ± 0.073
0.736ProHis: 0.736 ± 0.066
2.568ProIle: 2.568 ± 0.117
2.358ProLys: 2.358 ± 0.104
2.893ProLeu: 2.893 ± 0.106
0.732ProMet: 0.732 ± 0.056
1.43ProAsn: 1.43 ± 0.082
0.708ProPro: 0.708 ± 0.062
0.846ProGln: 0.846 ± 0.052
0.87ProArg: 0.87 ± 0.07
1.937ProSer: 1.937 ± 0.101
1.612ProThr: 1.612 ± 0.09
1.726ProVal: 1.726 ± 0.095
0.273ProTrp: 0.273 ± 0.039
1.301ProTyr: 1.301 ± 0.076
0.0ProXaa: 0.0 ± 0.0
Gln
2.071GlnAla: 2.071 ± 0.099
0.201GlnCys: 0.201 ± 0.029
1.717GlnAsp: 1.717 ± 0.091
2.563GlnGlu: 2.563 ± 0.132
1.181GlnPhe: 1.181 ± 0.09
1.296GlnGly: 1.296 ± 0.092
0.646GlnHis: 0.646 ± 0.053
2.558GlnIle: 2.558 ± 0.116
3.017GlnLys: 3.017 ± 0.127
2.487GlnLeu: 2.487 ± 0.099
0.947GlnMet: 0.947 ± 0.076
2.19GlnAsn: 2.19 ± 0.102
0.799GlnPro: 0.799 ± 0.063
0.956GlnGln: 0.956 ± 0.067
1.172GlnArg: 1.172 ± 0.076
1.889GlnSer: 1.889 ± 0.097
1.855GlnThr: 1.855 ± 0.106
1.583GlnVal: 1.583 ± 0.101
0.268GlnTrp: 0.268 ± 0.035
1.152GlnTyr: 1.152 ± 0.078
0.0GlnXaa: 0.0 ± 0.0
Arg
2.262ArgAla: 2.262 ± 0.098
0.268ArgCys: 0.268 ± 0.042
1.97ArgAsp: 1.97 ± 0.097
2.582ArgGlu: 2.582 ± 0.119
1.784ArgPhe: 1.784 ± 0.096
1.75ArgGly: 1.75 ± 0.115
0.603ArgHis: 0.603 ± 0.059
2.558ArgIle: 2.558 ± 0.117
2.372ArgLys: 2.372 ± 0.104
3.333ArgLeu: 3.333 ± 0.144
0.842ArgMet: 0.842 ± 0.074
1.487ArgAsn: 1.487 ± 0.081
0.837ArgPro: 0.837 ± 0.071
0.808ArgGln: 0.808 ± 0.061
1.296ArgArg: 1.296 ± 0.084
1.841ArgSer: 1.841 ± 0.103
1.64ArgThr: 1.64 ± 0.09
2.401ArgVal: 2.401 ± 0.111
0.239ArgTrp: 0.239 ± 0.033
1.363ArgTyr: 1.363 ± 0.079
0.0ArgXaa: 0.0 ± 0.0
Ser
4.404SerAla: 4.404 ± 0.166
0.497SerCys: 0.497 ± 0.045
4.026SerAsp: 4.026 ± 0.176
4.098SerGlu: 4.098 ± 0.135
3.653SerPhe: 3.653 ± 0.141
4.29SerGly: 4.29 ± 0.177
1.564SerHis: 1.564 ± 0.1
6.064SerIle: 6.064 ± 0.199
5.628SerLys: 5.628 ± 0.174
7.355SerLeu: 7.355 ± 0.203
1.965SerMet: 1.965 ± 0.088
3.304SerAsn: 3.304 ± 0.132
1.693SerPro: 1.693 ± 0.096
2.353SerGln: 2.353 ± 0.117
2.224SerArg: 2.224 ± 0.088
5.04SerSer: 5.04 ± 0.197
3.237SerThr: 3.237 ± 0.148
4.471SerVal: 4.471 ± 0.159
0.564SerTrp: 0.564 ± 0.062
2.85SerTyr: 2.85 ± 0.11
0.0SerXaa: 0.0 ± 0.0
Thr
3.309ThrAla: 3.309 ± 0.126
0.435ThrCys: 0.435 ± 0.049
2.845ThrAsp: 2.845 ± 0.128
2.549ThrGlu: 2.549 ± 0.11
2.444ThrPhe: 2.444 ± 0.132
3.094ThrGly: 3.094 ± 0.14
1.038ThrHis: 1.038 ± 0.059
4.38ThrIle: 4.38 ± 0.146
4.395ThrLys: 4.395 ± 0.146
5.758ThrLeu: 5.758 ± 0.174
1.205ThrMet: 1.205 ± 0.08
2.41ThrAsn: 2.41 ± 0.112
2.061ThrPro: 2.061 ± 0.096
2.099ThrGln: 2.099 ± 0.096
1.368ThrArg: 1.368 ± 0.082
3.338ThrSer: 3.338 ± 0.143
3.056ThrThr: 3.056 ± 0.129
3.185ThrVal: 3.185 ± 0.113
0.363ThrTrp: 0.363 ± 0.038
2.214ThrTyr: 2.214 ± 0.121
0.0ThrXaa: 0.0 ± 0.0
Val
4.572ValAla: 4.572 ± 0.181
0.555ValCys: 0.555 ± 0.057
3.634ValAsp: 3.634 ± 0.13
4.419ValGlu: 4.419 ± 0.129
2.821ValPhe: 2.821 ± 0.133
3.677ValGly: 3.677 ± 0.143
1.224ValHis: 1.224 ± 0.071
5.294ValIle: 5.294 ± 0.164
4.701ValLys: 4.701 ± 0.172
5.915ValLeu: 5.915 ± 0.17
1.564ValMet: 1.564 ± 0.094
3.166ValAsn: 3.166 ± 0.118
1.87ValPro: 1.87 ± 0.103
1.779ValGln: 1.779 ± 0.094
1.817ValArg: 1.817 ± 0.095
4.615ValSer: 4.615 ± 0.162
3.032ValThr: 3.032 ± 0.124
4.199ValVal: 4.199 ± 0.154
0.45ValTrp: 0.45 ± 0.049
2.061ValTyr: 2.061 ± 0.097
0.0ValXaa: 0.0 ± 0.0
Trp
0.416TrpAla: 0.416 ± 0.047
0.048TrpCys: 0.048 ± 0.016
0.359TrpAsp: 0.359 ± 0.041
0.473TrpGlu: 0.473 ± 0.044
0.421TrpPhe: 0.421 ± 0.04
0.426TrpGly: 0.426 ± 0.041
0.249TrpHis: 0.249 ± 0.039
0.727TrpIle: 0.727 ± 0.07
0.521TrpLys: 0.521 ± 0.05
0.717TrpLeu: 0.717 ± 0.057
0.292TrpMet: 0.292 ± 0.038
0.363TrpAsn: 0.363 ± 0.041
0.148TrpPro: 0.148 ± 0.033
0.263TrpGln: 0.263 ± 0.039
0.282TrpArg: 0.282 ± 0.035
0.392TrpSer: 0.392 ± 0.044
0.373TrpThr: 0.373 ± 0.051
0.445TrpVal: 0.445 ± 0.043
0.081TrpTrp: 0.081 ± 0.023
0.316TrpTyr: 0.316 ± 0.046
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.606TyrAla: 2.606 ± 0.121
0.301TyrCys: 0.301 ± 0.037
2.515TyrAsp: 2.515 ± 0.117
3.137TyrGlu: 3.137 ± 0.127
2.338TyrPhe: 2.338 ± 0.125
2.381TyrGly: 2.381 ± 0.098
0.789TyrHis: 0.789 ± 0.059
3.209TyrIle: 3.209 ± 0.134
3.668TyrLys: 3.668 ± 0.147
3.988TyrLeu: 3.988 ± 0.147
1.009TyrMet: 1.009 ± 0.073
2.209TyrAsn: 2.209 ± 0.124
1.176TyrPro: 1.176 ± 0.078
1.138TyrGln: 1.138 ± 0.071
1.258TyrArg: 1.258 ± 0.082
2.697TyrSer: 2.697 ± 0.129
2.2TyrThr: 2.2 ± 0.108
1.774TyrVal: 1.774 ± 0.081
0.296TyrTrp: 0.296 ± 0.033
1.612TyrTyr: 1.612 ± 0.112
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.005XaaGlu: 0.005 ± 0.004
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.005XaaAsn: 0.005 ± 0.005
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.014XaaXaa: 0.014 ± 0.013
Statistics based on 673 proteins (209116 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski