Amino acid dipepetide frequency for Lactobacillus phage JNU_P5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.225AlaAla: 4.225 ± 0.77
0.451AlaCys: 0.451 ± 0.196
4.957AlaAsp: 4.957 ± 0.601
4.225AlaGlu: 4.225 ± 0.524
2.479AlaPhe: 2.479 ± 0.345
5.014AlaGly: 5.014 ± 0.814
0.732AlaHis: 0.732 ± 0.195
5.014AlaIle: 5.014 ± 0.702
7.324AlaLys: 7.324 ± 0.957
5.971AlaLeu: 5.971 ± 0.521
2.76AlaMet: 2.76 ± 0.407
5.69AlaAsn: 5.69 ± 0.768
1.465AlaPro: 1.465 ± 0.211
4.338AlaGln: 4.338 ± 0.562
3.38AlaArg: 3.38 ± 0.533
6.479AlaSer: 6.479 ± 1.37
6.14AlaThr: 6.14 ± 0.997
4.45AlaVal: 4.45 ± 0.527
1.127AlaTrp: 1.127 ± 0.233
2.535AlaTyr: 2.535 ± 0.351
0.0AlaXaa: 0.0 ± 0.0
Cys
0.451CysAla: 0.451 ± 0.149
0.056CysCys: 0.056 ± 0.057
0.282CysAsp: 0.282 ± 0.161
0.394CysGlu: 0.394 ± 0.16
0.056CysPhe: 0.056 ± 0.057
0.451CysGly: 0.451 ± 0.225
0.169CysHis: 0.169 ± 0.086
0.451CysIle: 0.451 ± 0.194
0.169CysLys: 0.169 ± 0.094
0.394CysLeu: 0.394 ± 0.177
0.056CysMet: 0.056 ± 0.057
0.225CysAsn: 0.225 ± 0.121
0.169CysPro: 0.169 ± 0.117
0.225CysGln: 0.225 ± 0.13
0.225CysArg: 0.225 ± 0.135
0.056CysSer: 0.056 ± 0.056
0.282CysThr: 0.282 ± 0.139
0.507CysVal: 0.507 ± 0.197
0.0CysTrp: 0.0 ± 0.0
0.507CysTyr: 0.507 ± 0.165
0.0CysXaa: 0.0 ± 0.0
Asp
5.239AspAla: 5.239 ± 0.59
0.282AspCys: 0.282 ± 0.121
4.788AspAsp: 4.788 ± 0.704
3.605AspGlu: 3.605 ± 0.465
3.549AspPhe: 3.549 ± 0.443
4.507AspGly: 4.507 ± 0.645
0.958AspHis: 0.958 ± 0.287
3.718AspIle: 3.718 ± 0.399
5.352AspLys: 5.352 ± 0.534
5.352AspLeu: 5.352 ± 0.52
1.803AspMet: 1.803 ± 0.305
3.155AspAsn: 3.155 ± 0.422
2.422AspPro: 2.422 ± 0.427
3.042AspGln: 3.042 ± 0.352
1.859AspArg: 1.859 ± 0.292
3.267AspSer: 3.267 ± 0.42
4.901AspThr: 4.901 ± 0.629
4.338AspVal: 4.338 ± 0.671
0.901AspTrp: 0.901 ± 0.225
3.718AspTyr: 3.718 ± 0.488
0.0AspXaa: 0.0 ± 0.0
Glu
3.605GluAla: 3.605 ± 0.424
0.113GluCys: 0.113 ± 0.115
3.042GluAsp: 3.042 ± 0.479
3.042GluGlu: 3.042 ± 0.44
2.253GluPhe: 2.253 ± 0.445
2.76GluGly: 2.76 ± 0.49
1.239GluHis: 1.239 ± 0.348
3.38GluIle: 3.38 ± 0.427
4.338GluLys: 4.338 ± 0.567
5.352GluLeu: 5.352 ± 0.552
1.465GluMet: 1.465 ± 0.257
3.098GluAsn: 3.098 ± 0.391
1.521GluPro: 1.521 ± 0.355
2.366GluGln: 2.366 ± 0.369
3.324GluArg: 3.324 ± 0.439
3.943GluSer: 3.943 ± 0.541
3.549GluThr: 3.549 ± 0.471
3.324GluVal: 3.324 ± 0.52
0.676GluTrp: 0.676 ± 0.186
2.141GluTyr: 2.141 ± 0.496
0.0GluXaa: 0.0 ± 0.0
Phe
3.042PheAla: 3.042 ± 0.384
0.338PheCys: 0.338 ± 0.134
2.986PheAsp: 2.986 ± 0.402
1.746PheGlu: 1.746 ± 0.256
1.521PhePhe: 1.521 ± 0.31
2.422PheGly: 2.422 ± 0.386
0.676PheHis: 0.676 ± 0.181
2.873PheIle: 2.873 ± 0.543
2.929PheLys: 2.929 ± 0.425
2.76PheLeu: 2.76 ± 0.385
0.958PheMet: 0.958 ± 0.293
2.31PheAsn: 2.31 ± 0.417
1.352PhePro: 1.352 ± 0.291
1.127PheGln: 1.127 ± 0.245
1.634PheArg: 1.634 ± 0.326
2.817PheSer: 2.817 ± 0.466
3.098PheThr: 3.098 ± 0.371
2.197PheVal: 2.197 ± 0.381
0.563PheTrp: 0.563 ± 0.165
1.634PheTyr: 1.634 ± 0.322
0.0PheXaa: 0.0 ± 0.0
Gly
4.563GlyAla: 4.563 ± 0.627
0.169GlyCys: 0.169 ± 0.085
4.169GlyAsp: 4.169 ± 0.409
3.155GlyGlu: 3.155 ± 0.38
3.38GlyPhe: 3.38 ± 0.546
4.112GlyGly: 4.112 ± 0.851
0.845GlyHis: 0.845 ± 0.227
4.056GlyIle: 4.056 ± 0.613
5.915GlyLys: 5.915 ± 0.759
5.633GlyLeu: 5.633 ± 0.79
1.746GlyMet: 1.746 ± 0.315
3.718GlyAsn: 3.718 ± 0.512
1.127GlyPro: 1.127 ± 0.263
2.76GlyGln: 2.76 ± 0.374
1.69GlyArg: 1.69 ± 0.358
4.619GlySer: 4.619 ± 0.765
4.619GlyThr: 4.619 ± 0.618
3.155GlyVal: 3.155 ± 0.341
1.127GlyTrp: 1.127 ± 0.215
2.873GlyTyr: 2.873 ± 0.315
0.0GlyXaa: 0.0 ± 0.0
His
0.845HisAla: 0.845 ± 0.191
0.338HisCys: 0.338 ± 0.156
0.901HisAsp: 0.901 ± 0.211
1.07HisGlu: 1.07 ± 0.234
0.789HisPhe: 0.789 ± 0.228
1.239HisGly: 1.239 ± 0.295
1.014HisHis: 1.014 ± 0.314
0.676HisIle: 0.676 ± 0.184
0.62HisLys: 0.62 ± 0.195
1.014HisLeu: 1.014 ± 0.282
0.563HisMet: 0.563 ± 0.155
0.789HisAsn: 0.789 ± 0.243
0.62HisPro: 0.62 ± 0.206
1.07HisGln: 1.07 ± 0.278
0.789HisArg: 0.789 ± 0.258
1.803HisSer: 1.803 ± 0.415
0.507HisThr: 0.507 ± 0.206
0.789HisVal: 0.789 ± 0.233
0.507HisTrp: 0.507 ± 0.168
0.676HisTyr: 0.676 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
5.464IleAla: 5.464 ± 0.593
0.732IleCys: 0.732 ± 0.285
4.563IleAsp: 4.563 ± 0.469
3.324IleGlu: 3.324 ± 0.465
2.422IlePhe: 2.422 ± 0.51
4.676IleGly: 4.676 ± 0.779
1.127IleHis: 1.127 ± 0.264
3.662IleIle: 3.662 ± 0.623
6.14IleLys: 6.14 ± 0.606
3.098IleLeu: 3.098 ± 0.589
1.07IleMet: 1.07 ± 0.313
4.901IleAsn: 4.901 ± 0.515
1.915IlePro: 1.915 ± 0.316
2.986IleGln: 2.986 ± 0.533
2.028IleArg: 2.028 ± 0.319
4.732IleSer: 4.732 ± 0.619
4.619IleThr: 4.619 ± 0.588
2.929IleVal: 2.929 ± 0.429
0.732IleTrp: 0.732 ± 0.249
1.972IleTyr: 1.972 ± 0.393
0.0IleXaa: 0.0 ± 0.0
Lys
6.535LysAla: 6.535 ± 0.99
0.563LysCys: 0.563 ± 0.197
4.732LysAsp: 4.732 ± 0.596
4.619LysGlu: 4.619 ± 0.537
2.704LysPhe: 2.704 ± 0.442
3.887LysGly: 3.887 ± 0.54
1.465LysHis: 1.465 ± 0.314
4.112LysIle: 4.112 ± 0.556
6.535LysLys: 6.535 ± 0.979
7.042LysLeu: 7.042 ± 0.703
1.859LysMet: 1.859 ± 0.406
5.295LysAsn: 5.295 ± 0.689
2.31LysPro: 2.31 ± 0.367
4.394LysGln: 4.394 ± 0.513
3.267LysArg: 3.267 ± 0.504
6.253LysSer: 6.253 ± 0.954
5.126LysThr: 5.126 ± 0.637
3.887LysVal: 3.887 ± 0.461
1.07LysTrp: 1.07 ± 0.261
2.648LysTyr: 2.648 ± 0.4
0.0LysXaa: 0.0 ± 0.0
Leu
6.422LeuAla: 6.422 ± 0.684
0.113LeuCys: 0.113 ± 0.088
6.14LeuAsp: 6.14 ± 0.649
4.338LeuGlu: 4.338 ± 0.521
2.929LeuPhe: 2.929 ± 0.437
4.788LeuGly: 4.788 ± 0.934
1.239LeuHis: 1.239 ± 0.28
5.521LeuIle: 5.521 ± 0.775
6.591LeuLys: 6.591 ± 0.673
4.788LeuLeu: 4.788 ± 0.56
1.634LeuMet: 1.634 ± 0.351
5.07LeuAsn: 5.07 ± 0.68
2.535LeuPro: 2.535 ± 0.382
2.873LeuGln: 2.873 ± 0.44
2.986LeuArg: 2.986 ± 0.37
6.14LeuSer: 6.14 ± 0.591
5.859LeuThr: 5.859 ± 0.454
4.225LeuVal: 4.225 ± 0.545
0.563LeuTrp: 0.563 ± 0.196
2.535LeuTyr: 2.535 ± 0.524
0.0LeuXaa: 0.0 ± 0.0
Met
2.422MetAla: 2.422 ± 0.536
0.225MetCys: 0.225 ± 0.116
1.408MetAsp: 1.408 ± 0.276
1.69MetGlu: 1.69 ± 0.36
0.901MetPhe: 0.901 ± 0.22
1.014MetGly: 1.014 ± 0.231
0.282MetHis: 0.282 ± 0.149
1.915MetIle: 1.915 ± 0.358
2.366MetLys: 2.366 ± 0.364
1.634MetLeu: 1.634 ± 0.303
0.394MetMet: 0.394 ± 0.133
2.084MetAsn: 2.084 ± 0.313
0.676MetPro: 0.676 ± 0.261
1.239MetGln: 1.239 ± 0.255
1.127MetArg: 1.127 ± 0.286
1.239MetSer: 1.239 ± 0.233
2.197MetThr: 2.197 ± 0.362
1.183MetVal: 1.183 ± 0.364
0.225MetTrp: 0.225 ± 0.136
0.845MetTyr: 0.845 ± 0.249
0.0MetXaa: 0.0 ± 0.0
Asn
4.901AsnAla: 4.901 ± 1.063
0.282AsnCys: 0.282 ± 0.175
4.338AsnAsp: 4.338 ± 0.561
3.436AsnGlu: 3.436 ± 0.409
2.873AsnPhe: 2.873 ± 0.367
5.126AsnGly: 5.126 ± 0.498
0.676AsnHis: 0.676 ± 0.231
2.929AsnIle: 2.929 ± 0.466
3.493AsnLys: 3.493 ± 0.504
4.957AsnLeu: 4.957 ± 0.577
1.521AsnMet: 1.521 ± 0.293
3.718AsnAsn: 3.718 ± 0.465
2.31AsnPro: 2.31 ± 0.362
3.267AsnGln: 3.267 ± 0.504
2.929AsnArg: 2.929 ± 0.382
4.225AsnSer: 4.225 ± 0.552
3.436AsnThr: 3.436 ± 0.518
4.225AsnVal: 4.225 ± 0.373
0.845AsnTrp: 0.845 ± 0.202
2.366AsnTyr: 2.366 ± 0.358
0.0AsnXaa: 0.0 ± 0.0
Pro
2.084ProAla: 2.084 ± 0.419
0.056ProCys: 0.056 ± 0.044
1.972ProAsp: 1.972 ± 0.348
2.929ProGlu: 2.929 ± 0.575
1.239ProPhe: 1.239 ± 0.31
2.141ProGly: 2.141 ± 0.358
0.338ProHis: 0.338 ± 0.148
1.577ProIle: 1.577 ± 0.27
1.915ProLys: 1.915 ± 0.347
1.859ProLeu: 1.859 ± 0.441
0.732ProMet: 0.732 ± 0.167
1.239ProAsn: 1.239 ± 0.372
0.901ProPro: 0.901 ± 0.244
1.239ProGln: 1.239 ± 0.249
1.183ProArg: 1.183 ± 0.316
2.422ProSer: 2.422 ± 0.364
2.76ProThr: 2.76 ± 0.65
1.69ProVal: 1.69 ± 0.328
0.958ProTrp: 0.958 ± 0.224
0.732ProTyr: 0.732 ± 0.256
0.0ProXaa: 0.0 ± 0.0
Gln
5.126GlnAla: 5.126 ± 0.57
0.225GlnCys: 0.225 ± 0.105
2.873GlnAsp: 2.873 ± 0.436
2.084GlnGlu: 2.084 ± 0.42
1.634GlnPhe: 1.634 ± 0.348
2.648GlnGly: 2.648 ± 0.484
0.789GlnHis: 0.789 ± 0.196
3.436GlnIle: 3.436 ± 0.329
2.873GlnLys: 2.873 ± 0.375
4.507GlnLeu: 4.507 ± 0.5
0.845GlnMet: 0.845 ± 0.208
2.479GlnAsn: 2.479 ± 0.403
1.803GlnPro: 1.803 ± 0.386
1.972GlnGln: 1.972 ± 0.378
1.634GlnArg: 1.634 ± 0.294
3.887GlnSer: 3.887 ± 0.591
3.436GlnThr: 3.436 ± 0.828
2.929GlnVal: 2.929 ± 0.512
0.732GlnTrp: 0.732 ± 0.176
1.803GlnTyr: 1.803 ± 0.303
0.0GlnXaa: 0.0 ± 0.0
Arg
2.31ArgAla: 2.31 ± 0.419
0.282ArgCys: 0.282 ± 0.158
2.704ArgAsp: 2.704 ± 0.357
1.972ArgGlu: 1.972 ± 0.421
2.141ArgPhe: 2.141 ± 0.298
2.084ArgGly: 2.084 ± 0.454
0.732ArgHis: 0.732 ± 0.214
2.535ArgIle: 2.535 ± 0.335
2.76ArgLys: 2.76 ± 0.42
4.394ArgLeu: 4.394 ± 0.555
0.958ArgMet: 0.958 ± 0.19
3.155ArgAsn: 3.155 ± 0.395
1.183ArgPro: 1.183 ± 0.279
1.972ArgGln: 1.972 ± 0.402
2.422ArgArg: 2.422 ± 0.395
2.253ArgSer: 2.253 ± 0.399
2.084ArgThr: 2.084 ± 0.311
1.859ArgVal: 1.859 ± 0.289
0.507ArgTrp: 0.507 ± 0.216
1.521ArgTyr: 1.521 ± 0.234
0.0ArgXaa: 0.0 ± 0.0
Ser
6.197SerAla: 6.197 ± 1.301
0.113SerCys: 0.113 ± 0.092
5.408SerAsp: 5.408 ± 0.985
3.831SerGlu: 3.831 ± 0.409
2.648SerPhe: 2.648 ± 0.38
4.732SerGly: 4.732 ± 0.731
1.577SerHis: 1.577 ± 0.365
5.464SerIle: 5.464 ± 0.756
5.746SerLys: 5.746 ± 1.295
5.07SerLeu: 5.07 ± 0.509
2.141SerMet: 2.141 ± 0.437
4.563SerAsn: 4.563 ± 0.524
2.141SerPro: 2.141 ± 0.51
3.493SerGln: 3.493 ± 0.709
2.479SerArg: 2.479 ± 0.384
5.633SerSer: 5.633 ± 0.999
3.943SerThr: 3.943 ± 0.454
4.732SerVal: 4.732 ± 0.521
1.127SerTrp: 1.127 ± 0.269
2.535SerTyr: 2.535 ± 0.297
0.0SerXaa: 0.0 ± 0.0
Thr
7.098ThrAla: 7.098 ± 1.263
0.225ThrCys: 0.225 ± 0.108
3.943ThrAsp: 3.943 ± 0.501
3.493ThrGlu: 3.493 ± 0.533
1.577ThrPhe: 1.577 ± 0.339
4.845ThrGly: 4.845 ± 0.511
0.732ThrHis: 0.732 ± 0.251
4.732ThrIle: 4.732 ± 0.498
4.957ThrLys: 4.957 ± 0.587
4.45ThrLeu: 4.45 ± 0.464
1.408ThrMet: 1.408 ± 0.299
4.281ThrAsn: 4.281 ± 0.665
2.253ThrPro: 2.253 ± 0.44
3.493ThrGln: 3.493 ± 0.475
2.366ThrArg: 2.366 ± 0.362
5.183ThrSer: 5.183 ± 0.668
6.253ThrThr: 6.253 ± 0.905
5.521ThrVal: 5.521 ± 0.703
0.901ThrTrp: 0.901 ± 0.296
1.859ThrTyr: 1.859 ± 0.355
0.0ThrXaa: 0.0 ± 0.0
Val
4.788ValAla: 4.788 ± 0.644
0.169ValCys: 0.169 ± 0.137
4.169ValAsp: 4.169 ± 0.442
2.817ValGlu: 2.817 ± 0.401
1.69ValPhe: 1.69 ± 0.281
3.436ValGly: 3.436 ± 0.541
1.127ValHis: 1.127 ± 0.222
4.169ValIle: 4.169 ± 0.464
4.845ValLys: 4.845 ± 0.507
4.45ValLeu: 4.45 ± 0.599
1.746ValMet: 1.746 ± 0.304
3.831ValAsn: 3.831 ± 0.433
1.746ValPro: 1.746 ± 0.315
2.648ValGln: 2.648 ± 0.405
2.084ValArg: 2.084 ± 0.323
4.281ValSer: 4.281 ± 0.557
3.718ValThr: 3.718 ± 0.609
3.718ValVal: 3.718 ± 0.478
0.62ValTrp: 0.62 ± 0.174
2.253ValTyr: 2.253 ± 0.408
0.0ValXaa: 0.0 ± 0.0
Trp
0.789TrpAla: 0.789 ± 0.234
0.169TrpCys: 0.169 ± 0.08
1.239TrpAsp: 1.239 ± 0.247
0.507TrpGlu: 0.507 ± 0.152
0.451TrpPhe: 0.451 ± 0.123
0.732TrpGly: 0.732 ± 0.159
0.169TrpHis: 0.169 ± 0.108
0.789TrpIle: 0.789 ± 0.21
0.958TrpLys: 0.958 ± 0.266
1.69TrpLeu: 1.69 ± 0.347
0.507TrpMet: 0.507 ± 0.136
0.394TrpAsn: 0.394 ± 0.167
0.338TrpPro: 0.338 ± 0.143
0.845TrpGln: 0.845 ± 0.252
0.732TrpArg: 0.732 ± 0.164
0.845TrpSer: 0.845 ± 0.174
0.845TrpThr: 0.845 ± 0.203
0.845TrpVal: 0.845 ± 0.248
0.169TrpTrp: 0.169 ± 0.096
0.563TrpTyr: 0.563 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.648TyrAla: 2.648 ± 0.31
0.225TyrCys: 0.225 ± 0.121
2.141TyrAsp: 2.141 ± 0.366
2.253TyrGlu: 2.253 ± 0.473
1.746TyrPhe: 1.746 ± 0.274
2.817TyrGly: 2.817 ± 0.375
0.732TyrHis: 0.732 ± 0.222
2.084TyrIle: 2.084 ± 0.343
2.479TyrLys: 2.479 ± 0.483
2.873TyrLeu: 2.873 ± 0.504
0.845TyrMet: 0.845 ± 0.239
1.859TyrAsn: 1.859 ± 0.251
1.183TyrPro: 1.183 ± 0.3
2.366TyrGln: 2.366 ± 0.404
1.69TyrArg: 1.69 ± 0.341
3.493TyrSer: 3.493 ± 0.406
2.197TyrThr: 2.197 ± 0.294
1.859TyrVal: 1.859 ± 0.381
0.225TyrTrp: 0.225 ± 0.096
1.915TyrTyr: 1.915 ± 0.391
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (17752 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski