Myxoma virus (strain Lausanne) (MYXV)
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 158 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9Q8H7|Q9Q8H7_MYXVL M124R OS=Myxoma virus (strain Lausanne) OX=31530 GN=m124R PE=4 SV=1
MM1 pKa = 7.35 IVVAYY6 pKa = 8.95 MGLLFSFCSLSAYY19 pKa = 10.17 LLSVYY24 pKa = 9.97 KK25 pKa = 10.45 HH26 pKa = 5.6 QIKK29 pKa = 10.25 KK30 pKa = 10.04 CLHH33 pKa = 6.35 RR34 pKa = 11.84 PTKK37 pKa = 8.99 RR38 pKa = 11.84 TKK40 pKa = 10.34 CIRR43 pKa = 11.84 LNSITYY49 pKa = 8.92 SADD52 pKa = 4.05 DD53 pKa = 3.63 IVHH56 pKa = 6.42 QIPEE60 pKa = 4.45 TVEE63 pKa = 3.84 SDD65 pKa = 4.71 DD66 pKa = 4.49 EE67 pKa = 5.21 FDD69 pKa = 4.8 SEE71 pKa = 4.29 WSSDD75 pKa = 3.49 EE76 pKa = 4.72 DD77 pKa = 4.08 DD78 pKa = 4.5 GEE80 pKa = 4.43 VYY82 pKa = 10.7 EE83 pKa = 4.92 NYY85 pKa = 9.58 TSKK88 pKa = 11.1 SEE90 pKa = 4.17 NNFVARR96 pKa = 11.84 TDD98 pKa = 3.7 DD99 pKa = 4.08 DD100 pKa = 4.05 VAVDD104 pKa = 3.6 VLVEE108 pKa = 4.37 TEE110 pKa = 5.86 DD111 pKa = 3.73 EE112 pKa = 4.65 PNWDD116 pKa = 3.35 PTIYY120 pKa = 10.5 DD121 pKa = 3.7 ANTSNVYY128 pKa = 9.73 EE129 pKa = 4.46 IPDD132 pKa = 4.19 DD133 pKa = 4.31 GEE135 pKa = 4.45 SFDD138 pKa = 5.82 DD139 pKa = 3.82 VQIDD143 pKa = 4.09 RR144 pKa = 11.84 NVSDD148 pKa = 4.21 KK149 pKa = 11.26 KK150 pKa = 9.63 YY151 pKa = 8.56 FTYY154 pKa = 8.78 FTEE157 pKa = 4.53 TAVSS161 pKa = 3.4
Molecular weight: 18.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.931
IPC2_protein 4.012
IPC_protein 3.999
Toseland 3.795
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.91
Rodwell 3.834
Grimsley 3.706
Solomon 3.986
Lehninger 3.935
Nozaki 4.101
DTASelect 4.329
Thurlkill 3.834
EMBOSS 3.923
Sillero 4.126
Patrickios 2.676
IPC_peptide 3.973
IPC2_peptide 4.101
IPC2.peptide.svr19 4.021
Protein with the highest isoelectric point:
>tr|Q9Q8F7|Q9Q8F7_MYXVL M147R OS=Myxoma virus (strain Lausanne) OX=31530 GN=m147R PE=4 SV=1
MM1 pKa = 7.16 TVIKK5 pKa = 10.0 PSSRR9 pKa = 11.84 PRR11 pKa = 11.84 PRR13 pKa = 11.84 KK14 pKa = 9.51 NKK16 pKa = 8.96 NIKK19 pKa = 9.03 VNTYY23 pKa = 7.63 RR24 pKa = 11.84 TSAMDD29 pKa = 3.96 LSPGSVHH36 pKa = 6.8 EE37 pKa = 4.75 GIVYY41 pKa = 10.08 FKK43 pKa = 11.21 DD44 pKa = 3.99 GIFKK48 pKa = 10.5 VRR50 pKa = 11.84 LLGYY54 pKa = 9.5 EE55 pKa = 3.81 GHH57 pKa = 6.38 EE58 pKa = 4.83 CILLDD63 pKa = 3.78 YY64 pKa = 11.13 LNYY67 pKa = 10.59 RR68 pKa = 11.84 QDD70 pKa = 3.32 TLDD73 pKa = 3.44 RR74 pKa = 11.84 LKK76 pKa = 10.71 EE77 pKa = 3.94 RR78 pKa = 11.84 LVGRR82 pKa = 11.84 VIKK85 pKa = 9.83 TRR87 pKa = 11.84 VVRR90 pKa = 11.84 ADD92 pKa = 3.26 GLYY95 pKa = 9.44 VDD97 pKa = 4.96 LRR99 pKa = 11.84 RR100 pKa = 11.84 FFF102 pKa = 4.71
Molecular weight: 11.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.266
IPC2_protein 9.589
IPC_protein 9.999
Toseland 10.218
ProMoST 9.94
Dawson 10.423
Bjellqvist 10.116
Wikipedia 10.613
Rodwell 10.73
Grimsley 10.496
Solomon 10.467
Lehninger 10.423
Nozaki 10.204
DTASelect 10.101
Thurlkill 10.277
EMBOSS 10.628
Sillero 10.335
Patrickios 10.394
IPC_peptide 10.467
IPC2_peptide 8.887
IPC2.peptide.svr19 8.612
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
158
0
158
48407
32
2000
306.4
35.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.526 ± 0.119
2.148 ± 0.106
6.171 ± 0.116
5.572 ± 0.125
4.838 ± 0.138
4.156 ± 0.126
2.26 ± 0.074
7.613 ± 0.181
7.429 ± 0.204
9.057 ± 0.161
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.357 ± 0.074
5.782 ± 0.138
3.568 ± 0.115
2.21 ± 0.083
4.706 ± 0.15
7.098 ± 0.148
6.683 ± 0.144
7.801 ± 0.161
0.727 ± 0.056
5.293 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here