Sphingobium sp. C100

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4513 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W1SE53|W1SE53_9SPHN Uncharacterized protein OS=Sphingobium sp. C100 OX=1207055 GN=C100_02225 PE=4 SV=1
MM1 pKa = 7.38RR2 pKa = 11.84TTYY5 pKa = 10.97DD6 pKa = 3.14GATVRR11 pKa = 11.84LYY13 pKa = 10.95HH14 pKa = 6.71LGDD17 pKa = 4.57DD18 pKa = 4.35GGVTTLIYY26 pKa = 10.87GPLSEE31 pKa = 4.79ALRR34 pKa = 11.84VADD37 pKa = 3.71QQPAEE42 pKa = 4.1MQEE45 pKa = 4.27GLFIATDD52 pKa = 3.68NDD54 pKa = 3.33VVAYY58 pKa = 10.53LDD60 pKa = 5.52LIEE63 pKa = 4.0QQ64 pKa = 3.84

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W1S0R9|W1S0R9_9SPHN Carboxynorspermidine/carboxyspermidine decarboxylase OS=Sphingobium sp. C100 OX=1207055 GN=C100_14705 PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.18PFLALLLAILATPLAAQTALPPAPWADD29 pKa = 3.58RR30 pKa = 11.84QIPDD34 pKa = 3.09ARR36 pKa = 11.84QEE38 pKa = 4.17RR39 pKa = 11.84EE40 pKa = 3.89AKK42 pKa = 10.64ALMEE46 pKa = 4.55TIRR49 pKa = 11.84CLTCQSQSIADD60 pKa = 4.13SNASMAGDD68 pKa = 3.44MRR70 pKa = 11.84SQIRR74 pKa = 11.84EE75 pKa = 3.72RR76 pKa = 11.84IMAGEE81 pKa = 4.23RR82 pKa = 11.84PDD84 pKa = 5.6AIRR87 pKa = 11.84SWLIARR93 pKa = 11.84YY94 pKa = 8.98GDD96 pKa = 3.3WVSYY100 pKa = 10.39EE101 pKa = 3.87PTAAPILWPLWAAPLLLLSLGLLLLRR127 pKa = 11.84GRR129 pKa = 11.84IKK131 pKa = 10.45RR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84QQ136 pKa = 3.04

Molecular weight: 15.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4513

0

4513

1409744

26

2529

312.4

33.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.241 ± 0.052

0.798 ± 0.012

6.225 ± 0.027

5.121 ± 0.036

3.504 ± 0.018

8.862 ± 0.04

2.067 ± 0.018

5.15 ± 0.023

2.927 ± 0.028

9.928 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.02

2.517 ± 0.023

5.298 ± 0.031

3.3 ± 0.02

7.392 ± 0.037

5.235 ± 0.029

5.146 ± 0.025

6.922 ± 0.031

1.457 ± 0.016

2.269 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski