Tilapia lake virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Amnoonviridae; Tilapinevirus; Tilapia tilapinevirus

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142I800|A0A142I800_9VIRU Uncharacterized protein OS=Tilapia lake virus OX=1549864 PE=4 SV=1
MM1 pKa = 7.37SVADD5 pKa = 4.2YY6 pKa = 10.85LSSDD10 pKa = 3.63SDD12 pKa = 3.72SGAEE16 pKa = 3.97SSGCLVLRR24 pKa = 11.84SRR26 pKa = 11.84KK27 pKa = 8.93IKK29 pKa = 10.12KK30 pKa = 9.3GKK32 pKa = 9.5KK33 pKa = 8.26AASKK37 pKa = 10.05KK38 pKa = 10.17RR39 pKa = 11.84SWKK42 pKa = 9.18NEE44 pKa = 3.39RR45 pKa = 11.84YY46 pKa = 9.73GADD49 pKa = 3.24EE50 pKa = 4.28SGEE53 pKa = 4.41DD54 pKa = 3.54NIEE57 pKa = 3.82WGDD60 pKa = 3.92EE61 pKa = 3.88VDD63 pKa = 6.56LEE65 pKa = 4.72MDD67 pKa = 4.45DD68 pKa = 4.7CDD70 pKa = 4.28SAIPEE75 pKa = 4.08WARR78 pKa = 11.84VDD80 pKa = 4.36FNPKK84 pKa = 8.84NRR86 pKa = 11.84RR87 pKa = 11.84DD88 pKa = 3.85RR89 pKa = 11.84EE90 pKa = 4.03DD91 pKa = 4.82DD92 pKa = 3.81GQSDD96 pKa = 4.22LSRR99 pKa = 11.84FSEE102 pKa = 4.31DD103 pKa = 3.03FGKK106 pKa = 10.83KK107 pKa = 10.1SLDD110 pKa = 3.54VQSS113 pKa = 3.81

Molecular weight: 12.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142I7Z8|A0A142I7Z8_9VIRU Uncharacterized protein OS=Tilapia lake virus OX=1549864 PE=4 SV=1
MM1 pKa = 7.53SYY3 pKa = 10.78KK4 pKa = 9.97IGEE7 pKa = 4.42LEE9 pKa = 4.81RR10 pKa = 11.84IITRR14 pKa = 11.84KK15 pKa = 8.16NTLPKK20 pKa = 10.63DD21 pKa = 3.62SGSQTGLFHH30 pKa = 7.94RR31 pKa = 11.84LLLEE35 pKa = 4.54HH36 pKa = 6.79YY37 pKa = 10.19SGASNVWFFCATGFTPNTNGTTWIVLTSHH66 pKa = 6.64PTDD69 pKa = 3.64GGEE72 pKa = 4.59KK73 pKa = 10.64VPLKK77 pKa = 9.78WKK79 pKa = 10.55YY80 pKa = 9.41EE81 pKa = 4.03VSPGLPVRR89 pKa = 11.84RR90 pKa = 11.84VLAQEE95 pKa = 4.16GTAVRR100 pKa = 11.84GPKK103 pKa = 9.62GAYY106 pKa = 8.74LVKK109 pKa = 10.73GDD111 pKa = 3.85MHH113 pKa = 6.81LCSTTFYY120 pKa = 10.36TRR122 pKa = 11.84RR123 pKa = 11.84EE124 pKa = 3.89AKK126 pKa = 9.92YY127 pKa = 8.61WLCAPSPKK135 pKa = 9.64FPHH138 pKa = 4.8WTKK141 pKa = 10.79RR142 pKa = 11.84SALVTSTRR150 pKa = 11.84PLTEE154 pKa = 4.2LSRR157 pKa = 11.84VATYY161 pKa = 10.96LEE163 pKa = 5.11AISKK167 pKa = 10.21GATDD171 pKa = 5.04VNEE174 pKa = 4.41SWCSYY179 pKa = 9.81HH180 pKa = 7.4RR181 pKa = 11.84VGLVPIPKK189 pKa = 10.42GITFEE194 pKa = 4.28LL195 pKa = 4.17

Molecular weight: 21.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

3011

113

519

301.1

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.207 ± 0.735

2.989 ± 0.245

5.214 ± 0.449

6.011 ± 0.322

3.886 ± 0.363

6.476 ± 0.282

2.026 ± 0.233

4.417 ± 0.281

6.244 ± 0.369

10.096 ± 0.441

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.302

2.889 ± 0.297

4.218 ± 0.304

3.022 ± 0.244

6.808 ± 0.299

9.034 ± 0.662

5.679 ± 0.609

6.842 ± 0.267

1.362 ± 0.252

3.155 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski