Kern Canyon virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ledantevirus; Kern Canyon ledantevirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R1K7|A0A0D3R1K7_9RHAB Phosphoprotein OS=Kern Canyon virus OX=380433 PE=4 SV=1
MM1 pKa = 7.76SDD3 pKa = 3.81FRR5 pKa = 11.84DD6 pKa = 3.78KK7 pKa = 11.19VKK9 pKa = 10.82KK10 pKa = 10.23LAAKK14 pKa = 9.64DD15 pKa = 3.08IWDD18 pKa = 4.73SISKK22 pKa = 10.83LEE24 pKa = 3.88IDD26 pKa = 4.17EE27 pKa = 5.56DD28 pKa = 4.09EE29 pKa = 4.54EE30 pKa = 4.96STFLEE35 pKa = 4.44NEE37 pKa = 4.05EE38 pKa = 4.9KK39 pKa = 10.45PRR41 pKa = 11.84SDD43 pKa = 5.12DD44 pKa = 3.02NWTKK48 pKa = 11.03AEE50 pKa = 4.26EE51 pKa = 4.22PSEE54 pKa = 4.29VPAYY58 pKa = 10.04EE59 pKa = 4.31EE60 pKa = 4.16EE61 pKa = 4.62FDD63 pKa = 4.66TEE65 pKa = 5.33DD66 pKa = 3.6EE67 pKa = 4.48DD68 pKa = 5.2EE69 pKa = 5.52EE70 pKa = 4.14IAQACKK76 pKa = 10.56EE77 pKa = 4.31KK78 pKa = 10.96GPEE81 pKa = 3.91DD82 pKa = 3.86LLEE85 pKa = 4.66GGILDD90 pKa = 3.76LHH92 pKa = 6.9AGSEE96 pKa = 4.32FEE98 pKa = 6.5DD99 pKa = 5.66DD100 pKa = 3.85DD101 pKa = 4.27THH103 pKa = 8.47CLGLSDD109 pKa = 4.83PLEE112 pKa = 4.15LSIDD116 pKa = 3.76SQEE119 pKa = 4.14RR120 pKa = 11.84AQSSEE125 pKa = 3.68DD126 pKa = 3.69DD127 pKa = 3.93NNEE130 pKa = 3.85GVPMVLPRR138 pKa = 11.84ILLDD142 pKa = 3.52TVGSQEE148 pKa = 4.9DD149 pKa = 3.86LDD151 pKa = 4.21DD152 pKa = 3.66RR153 pKa = 11.84CYY155 pKa = 11.76AMLHH159 pKa = 5.35MAFKK163 pKa = 10.84KK164 pKa = 10.21LGWMLLPQTCHH175 pKa = 6.87PIDD178 pKa = 3.82EE179 pKa = 4.71ALHH182 pKa = 6.11LVSTPIRR189 pKa = 11.84VQRR192 pKa = 11.84SQISSVSDD200 pKa = 3.38NPPSLEE206 pKa = 3.76NSTSVNQGRR215 pKa = 11.84SSEE218 pKa = 4.32AAPQSNSAVRR228 pKa = 11.84FEE230 pKa = 4.88DD231 pKa = 3.55IIRR234 pKa = 11.84RR235 pKa = 11.84MEE237 pKa = 3.98TGDD240 pKa = 3.08IKK242 pKa = 10.93FPKK245 pKa = 10.05KK246 pKa = 10.1NGKK249 pKa = 10.39GYY251 pKa = 7.52MTLDD255 pKa = 3.52LGIPGLTPEE264 pKa = 5.8LIRR267 pKa = 11.84EE268 pKa = 4.45VIAITSDD275 pKa = 3.26EE276 pKa = 4.38ADD278 pKa = 5.05LISKK282 pKa = 10.26LLDD285 pKa = 3.29ILDD288 pKa = 3.63MKK290 pKa = 10.27EE291 pKa = 4.31YY292 pKa = 10.73IMTLCHH298 pKa = 6.34YY299 pKa = 8.95PP300 pKa = 3.25

Molecular weight: 33.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R1G9|A0A0D3R1G9_9RHAB GDP polyribonucleotidyltransferase OS=Kern Canyon virus OX=380433 PE=4 SV=1
MM1 pKa = 7.58LSKK4 pKa = 10.63LKK6 pKa = 10.7KK7 pKa = 10.02SFSKK11 pKa = 10.77KK12 pKa = 8.85EE13 pKa = 3.88SEE15 pKa = 4.1KK16 pKa = 10.41TVMIPEE22 pKa = 5.18GPPPDD27 pKa = 3.69YY28 pKa = 11.1YY29 pKa = 11.53GGFFYY34 pKa = 10.23PEE36 pKa = 3.96PSAPPDD42 pKa = 3.38EE43 pKa = 4.35LVKK46 pKa = 10.47TEE48 pKa = 3.96SLYY51 pKa = 11.06VKK53 pKa = 10.78AEE55 pKa = 3.85IVVKK59 pKa = 10.68SEE61 pKa = 3.99VPIKK65 pKa = 10.32EE66 pKa = 3.91MKK68 pKa = 10.35ALQEE72 pKa = 4.02ILAVWVDD79 pKa = 3.8KK80 pKa = 11.2NVSPVQQKK88 pKa = 10.33HH89 pKa = 5.84LDD91 pKa = 3.08TWFYY95 pKa = 11.34LCLGLHH101 pKa = 5.62VRR103 pKa = 11.84RR104 pKa = 11.84DD105 pKa = 3.51QDD107 pKa = 3.44CTYY110 pKa = 10.47TNMYY114 pKa = 9.04RR115 pKa = 11.84AAVDD119 pKa = 3.26MVVEE123 pKa = 4.58VNHH126 pKa = 6.4RR127 pKa = 11.84PKK129 pKa = 10.14TSSAIKK135 pKa = 9.58YY136 pKa = 9.97IPFEE140 pKa = 3.99QTFDD144 pKa = 3.59TIYY147 pKa = 10.77GGRR150 pKa = 11.84PCEE153 pKa = 3.66VSFKK157 pKa = 11.3NKK159 pKa = 8.96MVSSKK164 pKa = 10.84RR165 pKa = 11.84KK166 pKa = 7.36GTPAHH171 pKa = 6.82LLYY174 pKa = 10.71NQPLKK179 pKa = 10.92NGQSLPKK186 pKa = 9.79PVEE189 pKa = 3.64IFNGYY194 pKa = 8.44GVEE197 pKa = 5.24VKK199 pKa = 9.85LTDD202 pKa = 3.67NKK204 pKa = 10.0VHH206 pKa = 6.8EE207 pKa = 4.67ISLII211 pKa = 3.73

Molecular weight: 24.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3706

77

2129

617.7

70.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.263 ± 0.577

1.781 ± 0.279

6.557 ± 0.581

6.746 ± 0.974

4.452 ± 0.633

5.828 ± 0.365

2.563 ± 0.392

7.528 ± 0.576

7.501 ± 0.724

9.552 ± 0.817

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.386

4.155 ± 0.332

5.046 ± 0.489

2.914 ± 0.461

4.857 ± 0.48

7.879 ± 0.498

5.181 ± 0.352

5.505 ± 0.759

1.727 ± 0.212

3.427 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski