Haemophilus parahaemolyticus
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2159 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A377I3F2|A0A377I3F2_HAEPH Transferrin-binding protein 1 OS=Haemophilus parahaemolyticus OX=735 GN=tbpA_1 PE=3 SV=1
MM1 pKa = 7.33 YY2 pKa = 10.64 NLTEE6 pKa = 4.28 LRR8 pKa = 11.84 QEE10 pKa = 3.71 TDD12 pKa = 3.53 EE13 pKa = 5.12 IIEE16 pKa = 4.21 NLLADD21 pKa = 4.34 GSDD24 pKa = 3.56 PEE26 pKa = 3.88 ALYY29 pKa = 10.34 IIEE32 pKa = 4.34 HH33 pKa = 6.44 HH34 pKa = 6.26 VAHH37 pKa = 7.01 HH38 pKa = 7.11 DD39 pKa = 3.61 FDD41 pKa = 4.39 KK42 pKa = 11.43 LEE44 pKa = 4.14 KK45 pKa = 10.64 LVVDD49 pKa = 5.05 AYY51 pKa = 10.22 KK52 pKa = 10.62 LGYY55 pKa = 9.28 EE56 pKa = 3.62 ISEE59 pKa = 4.2 AEE61 pKa = 4.12 EE62 pKa = 4.08 VEE64 pKa = 3.78 EE65 pKa = 4.64 DD66 pKa = 3.73 GNVFFVCDD74 pKa = 3.38 IVSEE78 pKa = 4.39 INLNADD84 pKa = 5.5 LITAQQKK91 pKa = 9.93 EE92 pKa = 4.7 LLPLIEE98 pKa = 4.47 KK99 pKa = 10.59 AGAEE103 pKa = 4.09 YY104 pKa = 10.31 EE105 pKa = 4.15 GWGTYY110 pKa = 10.13 FEE112 pKa = 5.36 DD113 pKa = 4.8 PNGEE117 pKa = 3.97 DD118 pKa = 5.38 DD119 pKa = 4.69 EE120 pKa = 5.48 YY121 pKa = 11.7 GDD123 pKa = 4.17 DD124 pKa = 4.08 GEE126 pKa = 4.94 FFEE129 pKa = 5.57 EE130 pKa = 4.31 HH131 pKa = 7.54 DD132 pKa = 3.79 EE133 pKa = 5.38 DD134 pKa = 5.21 FDD136 pKa = 4.89 FDD138 pKa = 3.61 EE139 pKa = 4.78 EE140 pKa = 4.61 EE141 pKa = 4.42 IVRR144 pKa = 11.84 HH145 pKa = 5.18
Molecular weight: 16.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.795
IPC_protein 3.77
Toseland 3.579
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.617
Rodwell 3.592
Grimsley 3.49
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 3.999
Thurlkill 3.605
EMBOSS 3.63
Sillero 3.884
Patrickios 1.837
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|A0A369ZA00|A0A369ZA00_HAEPH 50S ribosomal protein L3 OS=Haemophilus parahaemolyticus OX=735 GN=rplC PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.91 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2159
0
2159
623779
29
4420
288.9
32.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.58 ± 0.083
0.947 ± 0.018
4.878 ± 0.044
6.857 ± 0.083
4.479 ± 0.044
6.55 ± 0.071
2.036 ± 0.026
6.765 ± 0.055
6.517 ± 0.047
10.266 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.428 ± 0.027
4.914 ± 0.063
3.607 ± 0.031
4.679 ± 0.058
4.38 ± 0.057
5.8 ± 0.044
5.311 ± 0.066
6.749 ± 0.07
1.131 ± 0.018
3.123 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here