Amino acid dipepetide frequency for Mycobacterium sp. MOTT36Y

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.662AlaAla: 20.662 ± 0.197
1.035AlaCys: 1.035 ± 0.022
8.277AlaAsp: 8.277 ± 0.08
7.827AlaGlu: 7.827 ± 0.083
3.439AlaPhe: 3.439 ± 0.046
12.038AlaGly: 12.038 ± 0.1
2.679AlaHis: 2.679 ± 0.041
5.343AlaIle: 5.343 ± 0.063
3.151AlaLys: 3.151 ± 0.056
13.057AlaLeu: 13.057 ± 0.094
2.997AlaMet: 2.997 ± 0.046
2.685AlaAsn: 2.685 ± 0.043
6.63AlaPro: 6.63 ± 0.088
4.216AlaGln: 4.216 ± 0.051
9.042AlaArg: 9.042 ± 0.09
6.096AlaSer: 6.096 ± 0.066
7.105AlaThr: 7.105 ± 0.072
11.258AlaVal: 11.258 ± 0.098
1.725AlaTrp: 1.725 ± 0.036
2.381AlaTyr: 2.381 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
1.122CysAla: 1.122 ± 0.027
0.098CysCys: 0.098 ± 0.008
0.574CysAsp: 0.574 ± 0.021
0.442CysGlu: 0.442 ± 0.017
0.226CysPhe: 0.226 ± 0.012
0.954CysGly: 0.954 ± 0.024
0.209CysHis: 0.209 ± 0.013
0.272CysIle: 0.272 ± 0.014
0.139CysLys: 0.139 ± 0.009
0.666CysLeu: 0.666 ± 0.019
0.138CysMet: 0.138 ± 0.009
0.167CysAsn: 0.167 ± 0.01
0.539CysPro: 0.539 ± 0.021
0.215CysGln: 0.215 ± 0.012
0.578CysArg: 0.578 ± 0.018
0.449CysSer: 0.449 ± 0.018
0.442CysThr: 0.442 ± 0.015
0.708CysVal: 0.708 ± 0.019
0.141CysTrp: 0.141 ± 0.009
0.201CysTyr: 0.201 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
7.934AspAla: 7.934 ± 0.076
0.457AspCys: 0.457 ± 0.016
4.306AspAsp: 4.306 ± 0.068
4.117AspGlu: 4.117 ± 0.058
1.837AspPhe: 1.837 ± 0.041
5.799AspGly: 5.799 ± 0.084
1.456AspHis: 1.456 ± 0.029
2.622AspIle: 2.622 ± 0.039
1.395AspLys: 1.395 ± 0.029
5.892AspLeu: 5.892 ± 0.068
1.041AspMet: 1.041 ± 0.025
1.228AspAsn: 1.228 ± 0.03
4.478AspPro: 4.478 ± 0.056
1.754AspGln: 1.754 ± 0.032
4.564AspArg: 4.564 ± 0.052
2.792AspSer: 2.792 ± 0.075
3.083AspThr: 3.083 ± 0.048
5.382AspVal: 5.382 ± 0.058
0.962AspTrp: 0.962 ± 0.025
1.532AspTyr: 1.532 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
6.201GluAla: 6.201 ± 0.076
0.37GluCys: 0.37 ± 0.016
2.462GluAsp: 2.462 ± 0.047
2.517GluGlu: 2.517 ± 0.049
1.835GluPhe: 1.835 ± 0.036
3.382GluGly: 3.382 ± 0.05
1.6GluHis: 1.6 ± 0.03
2.663GluIle: 2.663 ± 0.05
1.304GluLys: 1.304 ± 0.032
6.701GluLeu: 6.701 ± 0.074
1.078GluMet: 1.078 ± 0.03
1.081GluAsn: 1.081 ± 0.028
3.005GluPro: 3.005 ± 0.05
2.135GluGln: 2.135 ± 0.041
4.583GluArg: 4.583 ± 0.065
2.704GluSer: 2.704 ± 0.039
2.659GluThr: 2.659 ± 0.039
4.267GluVal: 4.267 ± 0.057
0.777GluTrp: 0.777 ± 0.019
1.079GluTyr: 1.079 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.002PheAla: 4.002 ± 0.049
0.328PheCys: 0.328 ± 0.013
2.496PheAsp: 2.496 ± 0.04
1.59PheGlu: 1.59 ± 0.033
1.001PhePhe: 1.001 ± 0.027
3.442PheGly: 3.442 ± 0.052
0.671PheHis: 0.671 ± 0.02
1.059PheIle: 1.059 ± 0.024
0.571PheLys: 0.571 ± 0.019
2.461PheLeu: 2.461 ± 0.044
0.522PheMet: 0.522 ± 0.018
0.754PheAsn: 0.754 ± 0.023
1.447PhePro: 1.447 ± 0.027
0.693PheGln: 0.693 ± 0.02
1.706PheArg: 1.706 ± 0.033
1.751PheSer: 1.751 ± 0.03
2.005PheThr: 2.005 ± 0.034
2.562PheVal: 2.562 ± 0.038
0.444PheTrp: 0.444 ± 0.016
0.736PheTyr: 0.736 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
10.367GlyAla: 10.367 ± 0.102
0.823GlyCys: 0.823 ± 0.021
5.018GlyAsp: 5.018 ± 0.061
4.43GlyGlu: 4.43 ± 0.055
2.979GlyPhe: 2.979 ± 0.045
8.0GlyGly: 8.0 ± 0.204
2.099GlyHis: 2.099 ± 0.042
4.061GlyIle: 4.061 ± 0.051
2.344GlyLys: 2.344 ± 0.045
8.605GlyLeu: 8.605 ± 0.078
2.21GlyMet: 2.21 ± 0.039
1.857GlyAsn: 1.857 ± 0.034
4.858GlyPro: 4.858 ± 0.061
2.927GlyGln: 2.927 ± 0.051
6.438GlyArg: 6.438 ± 0.074
5.164GlySer: 5.164 ± 0.094
5.084GlyThr: 5.084 ± 0.15
7.438GlyVal: 7.438 ± 0.084
1.681GlyTrp: 1.681 ± 0.036
2.418GlyTyr: 2.418 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
2.628HisAla: 2.628 ± 0.045
0.241HisCys: 0.241 ± 0.012
1.464HisAsp: 1.464 ± 0.034
1.127HisGlu: 1.127 ± 0.024
0.675HisPhe: 0.675 ± 0.019
2.183HisGly: 2.183 ± 0.031
0.767HisHis: 0.767 ± 0.028
0.855HisIle: 0.855 ± 0.022
0.382HisLys: 0.382 ± 0.016
2.147HisLeu: 2.147 ± 0.037
0.379HisMet: 0.379 ± 0.017
0.533HisAsn: 0.533 ± 0.018
1.725HisPro: 1.725 ± 0.034
0.697HisGln: 0.697 ± 0.02
1.977HisArg: 1.977 ± 0.037
1.155HisSer: 1.155 ± 0.026
1.251HisThr: 1.251 ± 0.029
1.799HisVal: 1.799 ± 0.031
0.42HisTrp: 0.42 ± 0.016
0.595HisTyr: 0.595 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.267IleAla: 6.267 ± 0.063
0.398IleCys: 0.398 ± 0.015
3.525IleAsp: 3.525 ± 0.053
2.658IleGlu: 2.658 ± 0.047
1.072IlePhe: 1.072 ± 0.026
4.345IleGly: 4.345 ± 0.045
0.866IleHis: 0.866 ± 0.021
1.395IleIle: 1.395 ± 0.03
0.935IleLys: 0.935 ± 0.027
2.94IleLeu: 2.94 ± 0.04
0.619IleMet: 0.619 ± 0.021
1.21IleAsn: 1.21 ± 0.03
2.409IlePro: 2.409 ± 0.035
0.938IleGln: 0.938 ± 0.025
2.68IleArg: 2.68 ± 0.041
2.266IleSer: 2.266 ± 0.039
2.821IleThr: 2.821 ± 0.042
3.657IleVal: 3.657 ± 0.054
0.467IleTrp: 0.467 ± 0.02
0.823IleTyr: 0.823 ± 0.024
0.0IleXaa: 0.0 ± 0.0
Lys
2.772LysAla: 2.772 ± 0.048
0.12LysCys: 0.12 ± 0.01
1.198LysAsp: 1.198 ± 0.027
0.983LysGlu: 0.983 ± 0.028
0.651LysPhe: 0.651 ± 0.02
1.591LysGly: 1.591 ± 0.038
0.531LysHis: 0.531 ± 0.02
1.05LysIle: 1.05 ± 0.025
0.684LysLys: 0.684 ± 0.028
2.282LysLeu: 2.282 ± 0.041
0.493LysMet: 0.493 ± 0.019
0.538LysAsn: 0.538 ± 0.022
1.498LysPro: 1.498 ± 0.037
0.707LysGln: 0.707 ± 0.022
1.673LysArg: 1.673 ± 0.031
1.273LysSer: 1.273 ± 0.032
1.397LysThr: 1.397 ± 0.033
1.903LysVal: 1.903 ± 0.039
0.33LysTrp: 0.33 ± 0.015
0.487LysTyr: 0.487 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
14.147LeuAla: 14.147 ± 0.107
0.838LeuCys: 0.838 ± 0.019
6.571LeuAsp: 6.571 ± 0.075
4.355LeuGlu: 4.355 ± 0.055
2.656LeuPhe: 2.656 ± 0.044
8.795LeuGly: 8.795 ± 0.08
2.108LeuHis: 2.108 ± 0.035
4.098LeuIle: 4.098 ± 0.057
1.862LeuLys: 1.862 ± 0.042
9.62LeuLeu: 9.62 ± 0.097
1.768LeuMet: 1.768 ± 0.035
2.12LeuAsn: 2.12 ± 0.04
5.832LeuPro: 5.832 ± 0.064
2.622LeuGln: 2.622 ± 0.047
7.777LeuArg: 7.777 ± 0.084
5.742LeuSer: 5.742 ± 0.068
6.258LeuThr: 6.258 ± 0.129
8.078LeuVal: 8.078 ± 0.07
1.231LeuTrp: 1.231 ± 0.028
1.703LeuTyr: 1.703 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
2.62MetAla: 2.62 ± 0.042
0.202MetCys: 0.202 ± 0.012
0.932MetAsp: 0.932 ± 0.025
0.711MetGlu: 0.711 ± 0.02
0.662MetPhe: 0.662 ± 0.022
1.556MetGly: 1.556 ± 0.032
0.446MetHis: 0.446 ± 0.015
0.917MetIle: 0.917 ± 0.022
0.48MetLys: 0.48 ± 0.021
2.133MetLeu: 2.133 ± 0.04
0.451MetMet: 0.451 ± 0.018
0.482MetAsn: 0.482 ± 0.018
1.282MetPro: 1.282 ± 0.026
0.539MetGln: 0.539 ± 0.017
1.588MetArg: 1.588 ± 0.031
1.524MetSer: 1.524 ± 0.035
1.785MetThr: 1.785 ± 0.034
1.709MetVal: 1.709 ± 0.033
0.292MetTrp: 0.292 ± 0.014
0.398MetTyr: 0.398 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.724AsnAla: 2.724 ± 0.048
0.176AsnCys: 0.176 ± 0.009
1.248AsnAsp: 1.248 ± 0.031
0.992AsnGlu: 0.992 ± 0.028
0.736AsnPhe: 0.736 ± 0.022
2.055AsnGly: 2.055 ± 0.047
0.528AsnHis: 0.528 ± 0.019
0.975AsnIle: 0.975 ± 0.026
0.491AsnLys: 0.491 ± 0.019
2.051AsnLeu: 2.051 ± 0.042
0.449AsnMet: 0.449 ± 0.016
0.576AsnAsn: 0.576 ± 0.018
1.769AsnPro: 1.769 ± 0.035
0.677AsnGln: 0.677 ± 0.022
1.614AsnArg: 1.614 ± 0.029
1.153AsnSer: 1.153 ± 0.029
1.271AsnThr: 1.271 ± 0.028
1.818AsnVal: 1.818 ± 0.036
0.406AsnTrp: 0.406 ± 0.015
0.59AsnTyr: 0.59 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
7.606ProAla: 7.606 ± 0.097
0.307ProCys: 0.307 ± 0.015
4.401ProAsp: 4.401 ± 0.05
3.719ProGlu: 3.719 ± 0.054
1.683ProPhe: 1.683 ± 0.035
5.912ProGly: 5.912 ± 0.07
1.301ProHis: 1.301 ± 0.027
2.249ProIle: 2.249 ± 0.044
1.341ProLys: 1.341 ± 0.027
4.958ProLeu: 4.958 ± 0.057
1.25ProMet: 1.25 ± 0.031
1.306ProAsn: 1.306 ± 0.029
4.068ProPro: 4.068 ± 0.094
1.922ProGln: 1.922 ± 0.035
3.614ProArg: 3.614 ± 0.048
3.148ProSer: 3.148 ± 0.043
3.366ProThr: 3.366 ± 0.044
4.947ProVal: 4.947 ± 0.062
0.859ProTrp: 0.859 ± 0.022
1.208ProTyr: 1.208 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.71GlnAla: 3.71 ± 0.051
0.228GlnCys: 0.228 ± 0.011
1.219GlnAsp: 1.219 ± 0.027
1.13GlnGlu: 1.13 ± 0.028
0.972GlnPhe: 0.972 ± 0.022
2.002GlnGly: 2.002 ± 0.034
0.823GlnHis: 0.823 ± 0.02
1.462GlnIle: 1.462 ± 0.034
0.618GlnLys: 0.618 ± 0.021
3.811GlnLeu: 3.811 ± 0.06
0.679GlnMet: 0.679 ± 0.021
0.642GlnAsn: 0.642 ± 0.02
1.897GlnPro: 1.897 ± 0.038
1.363GlnGln: 1.363 ± 0.036
2.902GlnArg: 2.902 ± 0.051
1.49GlnSer: 1.49 ± 0.03
1.62GlnThr: 1.62 ± 0.03
2.536GlnVal: 2.536 ± 0.046
0.548GlnTrp: 0.548 ± 0.019
0.672GlnTyr: 0.672 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
8.776ArgAla: 8.776 ± 0.094
0.61ArgCys: 0.61 ± 0.018
4.307ArgAsp: 4.307 ± 0.055
3.843ArgGlu: 3.843 ± 0.056
2.499ArgPhe: 2.499 ± 0.042
5.434ArgGly: 5.434 ± 0.06
1.903ArgHis: 1.903 ± 0.043
3.422ArgIle: 3.422 ± 0.047
1.621ArgLys: 1.621 ± 0.027
7.832ArgLeu: 7.832 ± 0.095
1.787ArgMet: 1.787 ± 0.037
1.645ArgAsn: 1.645 ± 0.031
4.184ArgPro: 4.184 ± 0.058
2.37ArgGln: 2.37 ± 0.042
6.798ArgArg: 6.798 ± 0.08
4.008ArgSer: 4.008 ± 0.047
3.993ArgThr: 3.993 ± 0.048
5.658ArgVal: 5.658 ± 0.061
1.434ArgTrp: 1.434 ± 0.034
1.961ArgTyr: 1.961 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
7.024SerAla: 7.024 ± 0.077
0.404SerCys: 0.404 ± 0.018
3.192SerAsp: 3.192 ± 0.047
2.578SerGlu: 2.578 ± 0.046
1.689SerPhe: 1.689 ± 0.039
5.736SerGly: 5.736 ± 0.141
1.086SerHis: 1.086 ± 0.027
2.144SerIle: 2.144 ± 0.034
1.17SerLys: 1.17 ± 0.03
4.805SerLeu: 4.805 ± 0.061
1.314SerMet: 1.314 ± 0.026
1.154SerAsn: 1.154 ± 0.029
3.13SerPro: 3.13 ± 0.04
1.505SerGln: 1.505 ± 0.032
3.767SerArg: 3.767 ± 0.05
3.144SerSer: 3.144 ± 0.062
3.14SerThr: 3.14 ± 0.049
4.484SerVal: 4.484 ± 0.062
0.931SerTrp: 0.931 ± 0.025
1.25SerTyr: 1.25 ± 0.029
0.0SerXaa: 0.0 ± 0.0
Thr
7.587ThrAla: 7.587 ± 0.071
0.424ThrCys: 0.424 ± 0.016
3.578ThrAsp: 3.578 ± 0.089
3.07ThrGlu: 3.07 ± 0.049
1.757ThrPhe: 1.757 ± 0.036
5.585ThrGly: 5.585 ± 0.067
1.181ThrHis: 1.181 ± 0.029
2.436ThrIle: 2.436 ± 0.043
1.302ThrLys: 1.302 ± 0.03
5.506ThrLeu: 5.506 ± 0.099
1.252ThrMet: 1.252 ± 0.025
1.297ThrAsn: 1.297 ± 0.029
3.723ThrPro: 3.723 ± 0.048
1.577ThrGln: 1.577 ± 0.033
3.598ThrArg: 3.598 ± 0.041
3.007ThrSer: 3.007 ± 0.048
3.728ThrThr: 3.728 ± 0.054
5.622ThrVal: 5.622 ± 0.147
0.854ThrTrp: 0.854 ± 0.023
1.269ThrTyr: 1.269 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
11.33ValAla: 11.33 ± 0.106
0.79ValCys: 0.79 ± 0.022
5.618ValAsp: 5.618 ± 0.073
4.476ValGlu: 4.476 ± 0.055
2.569ValPhe: 2.569 ± 0.04
6.877ValGly: 6.877 ± 0.068
1.843ValHis: 1.843 ± 0.032
3.981ValIle: 3.981 ± 0.045
1.675ValLys: 1.675 ± 0.036
8.588ValLeu: 8.588 ± 0.083
1.619ValMet: 1.619 ± 0.036
2.123ValAsn: 2.123 ± 0.046
4.607ValPro: 4.607 ± 0.061
2.101ValGln: 2.101 ± 0.037
5.914ValArg: 5.914 ± 0.067
4.637ValSer: 4.637 ± 0.06
5.304ValThr: 5.304 ± 0.092
8.293ValVal: 8.293 ± 0.089
1.049ValTrp: 1.049 ± 0.028
1.504ValTyr: 1.504 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.652TrpAla: 1.652 ± 0.037
0.177TrpCys: 0.177 ± 0.011
0.851TrpAsp: 0.851 ± 0.023
0.716TrpGlu: 0.716 ± 0.019
0.512TrpPhe: 0.512 ± 0.02
1.055TrpGly: 1.055 ± 0.03
0.382TrpHis: 0.382 ± 0.014
0.616TrpIle: 0.616 ± 0.019
0.341TrpLys: 0.341 ± 0.015
1.783TrpLeu: 1.783 ± 0.04
0.356TrpMet: 0.356 ± 0.015
0.404TrpAsn: 0.404 ± 0.017
0.826TrpPro: 0.826 ± 0.021
0.643TrpGln: 0.643 ± 0.02
1.329TrpArg: 1.329 ± 0.03
0.98TrpSer: 0.98 ± 0.029
0.889TrpThr: 0.889 ± 0.022
1.115TrpVal: 1.115 ± 0.027
0.323TrpTrp: 0.323 ± 0.016
0.306TrpTyr: 0.306 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.511TyrAla: 2.511 ± 0.043
0.253TyrCys: 0.253 ± 0.011
1.394TyrAsp: 1.394 ± 0.03
1.125TyrGlu: 1.125 ± 0.027
0.792TyrPhe: 0.792 ± 0.023
2.058TyrGly: 2.058 ± 0.042
0.522TyrHis: 0.522 ± 0.018
0.674TyrIle: 0.674 ± 0.019
0.353TyrLys: 0.353 ± 0.013
2.344TyrLeu: 2.344 ± 0.037
0.307TyrMet: 0.307 ± 0.014
0.496TyrAsn: 0.496 ± 0.016
1.261TyrPro: 1.261 ± 0.03
0.723TyrGln: 0.723 ± 0.02
1.941TyrArg: 1.941 ± 0.039
1.162TyrSer: 1.162 ± 0.03
1.153TyrThr: 1.153 ± 0.027
1.664TyrVal: 1.664 ± 0.038
0.382TyrTrp: 0.382 ± 0.016
0.519TyrTyr: 0.519 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5100 proteins (1677582 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski