marine actinobacterium PHSC20C1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; unclassified Actinomycetia

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2668 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4AGK1|A4AGK1_9ACTN Glutamate 5-kinase OS=marine actinobacterium PHSC20C1 OX=312284 GN=proB PE=3 SV=1
MM1 pKa = 7.26SRR3 pKa = 11.84SKK5 pKa = 10.63FFSLSTVAAAGVLAITLAGCSAPAEE30 pKa = 4.34DD31 pKa = 5.04AAPADD36 pKa = 3.97GTLGSLTVGATATPAGEE53 pKa = 3.75IVQFVIDD60 pKa = 4.07SGQAADD66 pKa = 5.24AGLEE70 pKa = 3.85LDD72 pKa = 3.29IVEE75 pKa = 4.53FTDD78 pKa = 3.65YY79 pKa = 7.69TTPNPALSEE88 pKa = 4.18GSLDD92 pKa = 3.55VNLFQHH98 pKa = 6.59EE99 pKa = 4.56PFLDD103 pKa = 4.4LYY105 pKa = 11.47NEE107 pKa = 4.12NTGDD111 pKa = 3.55NLFVVGQVYY120 pKa = 9.94LPPLALYY127 pKa = 10.54SKK129 pKa = 9.54TVDD132 pKa = 3.34EE133 pKa = 5.4LADD136 pKa = 4.09LKK138 pKa = 11.27DD139 pKa = 3.74GANIALPNDD148 pKa = 3.33ASNEE152 pKa = 3.62GRR154 pKa = 11.84ALLLLADD161 pKa = 4.43AGIIEE166 pKa = 4.6TTDD169 pKa = 3.4TPSTVADD176 pKa = 3.54ITANPHH182 pKa = 6.65DD183 pKa = 4.49YY184 pKa = 11.45NFIEE188 pKa = 5.16IDD190 pKa = 3.53AASLPSALDD199 pKa = 3.75DD200 pKa = 3.46QDD202 pKa = 3.84AAIVNFNYY210 pKa = 10.53AGAAGLTGDD219 pKa = 4.26LQLVTEE225 pKa = 4.83PTTSSYY231 pKa = 11.71YY232 pKa = 8.73NTLVTRR238 pKa = 11.84DD239 pKa = 3.84EE240 pKa = 4.64LKK242 pKa = 10.62SDD244 pKa = 3.33PRR246 pKa = 11.84VLKK249 pKa = 10.58LYY251 pKa = 10.5EE252 pKa = 4.69LLTSDD257 pKa = 3.55EE258 pKa = 4.12TKK260 pKa = 10.62AYY262 pKa = 9.88INEE265 pKa = 4.73FYY267 pKa = 10.62AGLVIPVSS275 pKa = 3.57

Molecular weight: 28.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4AIY5|A4AIY5_9ACTN Thiol:disulfide interchange protein OS=marine actinobacterium PHSC20C1 OX=312284 GN=A20C1_11271 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2668

0

2668

851365

22

6077

319.1

34.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.478 ± 0.054

0.484 ± 0.012

5.856 ± 0.039

5.778 ± 0.052

3.408 ± 0.033

8.36 ± 0.045

1.928 ± 0.031

5.384 ± 0.033

2.6 ± 0.039

10.078 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.93 ± 0.023

2.686 ± 0.032

4.849 ± 0.035

2.904 ± 0.022

6.231 ± 0.057

6.66 ± 0.044

6.277 ± 0.078

8.662 ± 0.041

1.394 ± 0.018

2.054 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski