Rathayibacter sp. Leaf296

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Rathayibacter; unclassified Rathayibacter

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3537 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5B832|A0A0Q5B832_9MICO Acetyltransferase OS=Rathayibacter sp. Leaf296 OX=1736327 GN=ASF46_13405 PE=4 SV=1
MM1 pKa = 7.8KK2 pKa = 9.97IRR4 pKa = 11.84RR5 pKa = 11.84FGLGALALVAASTLVLAGCSSSNDD29 pKa = 3.69DD30 pKa = 4.35GGGDD34 pKa = 3.62AAGDD38 pKa = 3.67SAAIILTNGSEE49 pKa = 4.23PQNPLVPTNTNEE61 pKa = 3.92VGGGKK66 pKa = 9.3ILDD69 pKa = 4.15EE70 pKa = 4.46IFAGLYY76 pKa = 10.44YY77 pKa = 10.61YY78 pKa = 10.52DD79 pKa = 4.87AEE81 pKa = 4.68GAAQPDD87 pKa = 3.76LAEE90 pKa = 4.45SVEE93 pKa = 4.63SDD95 pKa = 3.18DD96 pKa = 3.87AQNYY100 pKa = 7.27TITIEE105 pKa = 4.06SGRR108 pKa = 11.84TFTNGEE114 pKa = 4.31EE115 pKa = 3.71ITAQSFVDD123 pKa = 3.45AWNYY127 pKa = 7.51GAKK130 pKa = 10.22YY131 pKa = 10.63SNEE134 pKa = 3.64QLQSYY139 pKa = 8.54FFEE142 pKa = 6.24DD143 pKa = 3.26IEE145 pKa = 4.47GFSYY149 pKa = 11.31DD150 pKa = 4.23EE151 pKa = 4.3DD152 pKa = 4.4TEE154 pKa = 4.38LTGLKK159 pKa = 10.31VVDD162 pKa = 3.79DD163 pKa = 4.05TTFTVALTAPKK174 pKa = 10.33SDD176 pKa = 3.26FPQRR180 pKa = 11.84LGYY183 pKa = 8.89SAYY186 pKa = 10.6YY187 pKa = 8.77PLPSVAYY194 pKa = 10.2DD195 pKa = 3.51DD196 pKa = 3.86MDD198 pKa = 4.43AFGEE202 pKa = 4.15NPIGNGPYY210 pKa = 9.65MLDD213 pKa = 4.94GEE215 pKa = 5.11GAWQHH220 pKa = 6.42DD221 pKa = 4.06VQIDD225 pKa = 3.87LVVNPDD231 pKa = 3.1YY232 pKa = 11.1DD233 pKa = 3.98GPRR236 pKa = 11.84VPVNGGITITFYY248 pKa = 10.33ATQDD252 pKa = 2.86ASYY255 pKa = 11.65ADD257 pKa = 4.25LLAGNVDD264 pKa = 4.58VIDD267 pKa = 4.71QIPDD271 pKa = 3.29SAFGTFEE278 pKa = 4.19SDD280 pKa = 3.4LGDD283 pKa = 3.4RR284 pKa = 11.84AVNQASAVFQSFTIPEE300 pKa = 4.15RR301 pKa = 11.84LEE303 pKa = 4.16HH304 pKa = 6.82FSGEE308 pKa = 4.03EE309 pKa = 3.73GKK311 pKa = 10.49LRR313 pKa = 11.84RR314 pKa = 11.84QAISMAFDD322 pKa = 3.58RR323 pKa = 11.84EE324 pKa = 4.2QITDD328 pKa = 3.84VIFEE332 pKa = 4.27GTRR335 pKa = 11.84TPASDD340 pKa = 3.36FTSPVIDD347 pKa = 3.67GWSDD351 pKa = 3.32SLEE354 pKa = 4.07GAEE357 pKa = 3.84VLEE360 pKa = 4.3YY361 pKa = 11.26NPDD364 pKa = 3.47EE365 pKa = 4.61AKK367 pKa = 10.84KK368 pKa = 10.38LWAEE372 pKa = 4.09ADD374 pKa = 4.46AISPWTGTFSIAYY387 pKa = 8.93NADD390 pKa = 3.37GGHH393 pKa = 5.87QGWVDD398 pKa = 3.36AVSNAVKK405 pKa = 9.3NTLGIDD411 pKa = 3.87AVGEE415 pKa = 4.08AYY417 pKa = 7.62PTFAEE422 pKa = 4.12ARR424 pKa = 11.84TKK426 pKa = 10.45IVDD429 pKa = 3.65RR430 pKa = 11.84SIEE433 pKa = 4.09TAFRR437 pKa = 11.84TGWQADD443 pKa = 3.65YY444 pKa = 10.71PGLYY448 pKa = 10.33NFLGPLYY455 pKa = 9.65ATNASSNDD463 pKa = 3.02GDD465 pKa = 3.9YY466 pKa = 11.5SNPEE470 pKa = 3.85FDD472 pKa = 4.7QLLADD477 pKa = 5.07GSAEE481 pKa = 4.04TDD483 pKa = 3.15LATANEE489 pKa = 5.01DD490 pKa = 3.44YY491 pKa = 11.16QKK493 pKa = 11.05AQEE496 pKa = 4.51VLLQDD501 pKa = 4.65LPAIPLWYY509 pKa = 10.55ANVTGGYY516 pKa = 9.21SDD518 pKa = 3.73TVSDD522 pKa = 6.05VEE524 pKa = 4.56FGWNSVPLFYY534 pKa = 10.77QITKK538 pKa = 10.24SS539 pKa = 3.29

Molecular weight: 58.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5B367|A0A0Q5B367_9MICO Release factor glutamine methyltransferase OS=Rathayibacter sp. Leaf296 OX=1736327 GN=prmC PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84GILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.56GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3537

0

3537

1140147

29

2420

322.3

34.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.697 ± 0.061

0.483 ± 0.009

6.104 ± 0.039

5.885 ± 0.047

3.028 ± 0.022

9.09 ± 0.036

1.844 ± 0.019

4.044 ± 0.033

1.615 ± 0.029

10.717 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.502 ± 0.016

1.69 ± 0.02

5.484 ± 0.029

2.472 ± 0.023

7.55 ± 0.05

6.078 ± 0.031

6.084 ± 0.046

9.287 ± 0.039

1.424 ± 0.019

1.921 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski