Anopheles minimus irodovirus
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 131 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8QF18|W8QF18_9VIRU Uncharacterized protein OS=Anopheles minimus irodovirus OX=1465751 GN=AMIV_061 PE=4 SV=1
MM1 pKa = 7.07 LHH3 pKa = 6.84 SVFKK7 pKa = 10.69 DD8 pKa = 3.53 LYY10 pKa = 9.53 DD11 pKa = 3.41 QGVLIFLDD19 pKa = 3.21 TDD21 pKa = 3.5 NYY23 pKa = 8.62 VTNTCDD29 pKa = 4.36 DD30 pKa = 2.98 VDD32 pKa = 3.85 YY33 pKa = 10.52 FANRR37 pKa = 11.84 DD38 pKa = 3.92 KK39 pKa = 10.01 FTQMYY44 pKa = 8.13 TEE46 pKa = 5.21 PIKK49 pKa = 11.04 VGWIWSYY56 pKa = 11.61 APSLDD61 pKa = 3.41 EE62 pKa = 4.27 VVRR65 pKa = 11.84 TLKK68 pKa = 10.71 EE69 pKa = 3.91 DD70 pKa = 3.09 TCYY73 pKa = 10.86 LIEE76 pKa = 4.91 FGFNSVNEE84 pKa = 4.19 KK85 pKa = 10.36 KK86 pKa = 10.48 ALEE89 pKa = 4.07 VGRR92 pKa = 11.84 MLVNSLSKK100 pKa = 10.92 FEE102 pKa = 6.69 FMAQWNEE109 pKa = 3.64 RR110 pKa = 11.84 ALNEE114 pKa = 4.3 HH115 pKa = 6.91 KK116 pKa = 10.29 ISTVITSEE124 pKa = 4.1 NLPQSVQDD132 pKa = 3.91 LLEE135 pKa = 4.42 EE136 pKa = 4.49 YY137 pKa = 10.31 EE138 pKa = 4.51 LEE140 pKa = 4.18
Molecular weight: 16.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.342
IPC2_protein 4.368
IPC_protein 4.291
Toseland 4.113
ProMoST 4.355
Dawson 4.24
Bjellqvist 4.431
Wikipedia 4.113
Rodwell 4.126
Grimsley 4.024
Solomon 4.228
Lehninger 4.19
Nozaki 4.355
DTASelect 4.507
Thurlkill 4.139
EMBOSS 4.139
Sillero 4.393
Patrickios 3.465
IPC_peptide 4.24
IPC2_peptide 4.393
IPC2.peptide.svr19 4.317
Protein with the highest isoelectric point:
>tr|W8QF37|W8QF37_9VIRU Uncharacterized protein OS=Anopheles minimus irodovirus OX=1465751 GN=AMIV_086 PE=4 SV=1
MM1 pKa = 6.99 STFYY5 pKa = 10.61 PPRR8 pKa = 11.84 QKK10 pKa = 10.27 IRR12 pKa = 11.84 FSQKK16 pKa = 9.74 IIIIFVDD23 pKa = 3.18 KK24 pKa = 10.47 EE25 pKa = 3.94 DD26 pKa = 4.58 RR27 pKa = 11.84 RR28 pKa = 11.84 GPWEE32 pKa = 3.61 MLARR36 pKa = 11.84 DD37 pKa = 3.76 RR38 pKa = 11.84 DD39 pKa = 3.99 RR40 pKa = 11.84 FHH42 pKa = 8.31 HH43 pKa = 7.19 RR44 pKa = 11.84 IQNINNEE51 pKa = 4.24 IGWIFSPTHH60 pKa = 4.8 RR61 pKa = 11.84 TKK63 pKa = 10.32 IVKK66 pKa = 9.77 RR67 pKa = 11.84 DD68 pKa = 3.55 NLL70 pKa = 3.72
Molecular weight: 8.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.692
IPC_protein 10.716
Toseland 11.052
ProMoST 11.038
Dawson 11.096
Bjellqvist 10.877
Wikipedia 11.374
Rodwell 11.213
Grimsley 11.125
Solomon 11.359
Lehninger 11.301
Nozaki 11.038
DTASelect 10.877
Thurlkill 11.038
EMBOSS 11.491
Sillero 11.052
Patrickios 10.979
IPC_peptide 11.374
IPC2_peptide 9.911
IPC2.peptide.svr19 8.858
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
131
0
131
40583
42
1337
309.8
35.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.517 ± 0.168
1.782 ± 0.127
5.598 ± 0.125
6.665 ± 0.287
5.071 ± 0.144
4.899 ± 0.254
1.942 ± 0.084
6.946 ± 0.152
9.27 ± 0.272
9.43 ± 0.233
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.144 ± 0.089
6.074 ± 0.122
4.078 ± 0.156
3.925 ± 0.097
3.824 ± 0.147
6.867 ± 0.198
5.869 ± 0.23
6.035 ± 0.145
0.899 ± 0.051
4.164 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here