Ralstonia phage RS-PII-1
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L7DQE6|A0A1L7DQE6_9CAUD DNA packaging protein OS=Ralstonia phage RS-PII-1 OX=1932892 PE=4 SV=1
MM1 pKa = 7.31 SRR3 pKa = 11.84 DD4 pKa = 3.41 ISNMDD9 pKa = 4.44 DD10 pKa = 3.75 IIDD13 pKa = 3.53 VRR15 pKa = 11.84 QVIEE19 pKa = 3.99 RR20 pKa = 11.84 VEE22 pKa = 3.93 EE23 pKa = 3.88 LRR25 pKa = 11.84 EE26 pKa = 3.72 QRR28 pKa = 11.84 TPRR31 pKa = 11.84 FIVGYY36 pKa = 9.4 NLAGYY41 pKa = 8.55 MPDD44 pKa = 3.59 ADD46 pKa = 4.1 PGEE49 pKa = 4.31 FGDD52 pKa = 3.63 WGDD55 pKa = 3.73 AQRR58 pKa = 11.84 EE59 pKa = 4.23 LIDD62 pKa = 3.14 ILKK65 pKa = 10.32 RR66 pKa = 11.84 FEE68 pKa = 4.5 DD69 pKa = 4.0 EE70 pKa = 4.01 EE71 pKa = 4.94 GIPEE75 pKa = 4.25 GDD77 pKa = 3.19 AAEE80 pKa = 4.3 YY81 pKa = 10.28 CHH83 pKa = 6.87 AAEE86 pKa = 5.94 DD87 pKa = 3.85 VNLWSEE93 pKa = 4.2 IDD95 pKa = 4.92 DD96 pKa = 4.4 GDD98 pKa = 4.55 DD99 pKa = 4.33 DD100 pKa = 5.08 LVIHH104 pKa = 7.02 AGLYY108 pKa = 9.9 AFWIRR113 pKa = 11.84 RR114 pKa = 11.84 DD115 pKa = 3.22 GFMGLDD121 pKa = 3.1 SDD123 pKa = 4.91 EE124 pKa = 4.4 EE125 pKa = 4.48 NEE127 pKa = 4.57 LEE129 pKa = 4.64 LLEE132 pKa = 4.88 EE133 pKa = 4.62 LLSDD137 pKa = 4.12 LAGYY141 pKa = 10.22 GGDD144 pKa = 4.19 HH145 pKa = 5.57 QWEE148 pKa = 4.53 GDD150 pKa = 3.72 WYY152 pKa = 9.21 PVTLIRR158 pKa = 11.84 DD159 pKa = 4.0 SYY161 pKa = 9.16 FQTYY165 pKa = 8.68 AQEE168 pKa = 3.92 FAEE171 pKa = 5.2 DD172 pKa = 3.32 IGAVEE177 pKa = 6.19 HH178 pKa = 7.27 DD179 pKa = 4.01 AQWPNNCIDD188 pKa = 3.37 WEE190 pKa = 4.21 QAARR194 pKa = 11.84 EE195 pKa = 4.03 LRR197 pKa = 11.84 MDD199 pKa = 3.71 YY200 pKa = 11.11 SSVDD204 pKa = 4.12 FDD206 pKa = 5.67 GEE208 pKa = 4.8 TYY210 pKa = 9.79 WYY212 pKa = 10.48 RR213 pKa = 3.59
Molecular weight: 24.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.986
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.592
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.719
Sillero 3.923
Patrickios 2.943
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|A0A1L7DQN7|A0A1L7DQN7_9CAUD Uncharacterized protein OS=Ralstonia phage RS-PII-1 OX=1932892 PE=4 SV=1
MM1 pKa = 7.83 SIEE4 pKa = 4.43 EE5 pKa = 4.23 VNQLLRR11 pKa = 11.84 DD12 pKa = 3.61 MAVPHH17 pKa = 6.04 MLEE20 pKa = 3.59 TAYY23 pKa = 10.73 LRR25 pKa = 11.84 NIWRR29 pKa = 11.84 KK30 pKa = 8.66 PFYY33 pKa = 9.53 GTAFGVRR40 pKa = 11.84 RR41 pKa = 11.84 SRR43 pKa = 3.65
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.281
IPC2_protein 9.648
IPC_protein 10.599
Toseland 10.189
ProMoST 10.804
Dawson 10.438
Bjellqvist 10.233
Wikipedia 10.73
Rodwell 10.452
Grimsley 10.555
Solomon 10.57
Lehninger 10.526
Nozaki 10.145
DTASelect 10.233
Thurlkill 10.277
EMBOSS 10.613
Sillero 10.365
Patrickios 10.35
IPC_peptide 10.555
IPC2_peptide 9.238
IPC2.peptide.svr19 8.65
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
12847
43
1689
279.3
30.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.47 ± 0.603
0.833 ± 0.131
6.165 ± 0.213
5.916 ± 0.351
3.293 ± 0.198
7.971 ± 0.339
2.032 ± 0.147
4.203 ± 0.197
4.873 ± 0.308
8.36 ± 0.339
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.592 ± 0.177
3.892 ± 0.251
4.445 ± 0.233
4.382 ± 0.284
6.157 ± 0.378
5.402 ± 0.311
5.869 ± 0.333
6.624 ± 0.32
1.565 ± 0.139
2.958 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here