Synechococcus phage S-B68

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482IGF0|A0A482IGF0_9CAUD Uncharacterized protein OS=Synechococcus phage S-B68 OX=2545437 PE=4 SV=1
MM1 pKa = 7.99AITFPNSPSQGDD13 pKa = 3.8TYY15 pKa = 10.59TAEE18 pKa = 4.06NGLSYY23 pKa = 10.56EE24 pKa = 4.08YY25 pKa = 10.81DD26 pKa = 3.32GTKK29 pKa = 7.54WTTVSATTPAAYY41 pKa = 8.81WDD43 pKa = 3.88RR44 pKa = 11.84TGTTLSPAATGDD56 pKa = 3.77VVQVSAGTAALPGLTPVGDD75 pKa = 4.02TDD77 pKa = 3.51TGLYY81 pKa = 10.43APAANEE87 pKa = 4.16LAVSTAGSEE96 pKa = 4.29SFRR99 pKa = 11.84VDD101 pKa = 2.68ASGYY105 pKa = 9.46LLVGATTASGTANLQVTGGATIGGTTVLNDD135 pKa = 3.35GPTINGGATLNGATSFGAGVSGTEE159 pKa = 3.78QTITAGSFDD168 pKa = 4.68LDD170 pKa = 3.55NGNFWTVGAVAIPNPTNAVAGISGLIRR197 pKa = 11.84ITAAPTSWGSNFKK210 pKa = 10.8FPGGTAPSLASFPAMIAFYY229 pKa = 10.8VQDD232 pKa = 3.48STNILMGTAAEE243 pKa = 5.35GIAA246 pKa = 4.04

Molecular weight: 24.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482IH78|A0A482IH78_9CAUD Sliding clamp DNA polymerase accessory protein OS=Synechococcus phage S-B68 OX=2545437 PE=4 SV=1
MM1 pKa = 6.83FRR3 pKa = 11.84KK4 pKa = 9.68RR5 pKa = 11.84RR6 pKa = 11.84ALHH9 pKa = 5.94KK10 pKa = 10.64CPHH13 pKa = 6.71CEE15 pKa = 3.61MKK17 pKa = 10.47RR18 pKa = 11.84YY19 pKa = 8.08TGEE22 pKa = 3.81SLEE25 pKa = 4.75RR26 pKa = 11.84IPAHH30 pKa = 5.01MWYY33 pKa = 9.34IHH35 pKa = 6.88GIPSYY40 pKa = 9.45MQINGKK46 pKa = 7.78TIYY49 pKa = 10.26SGPTGPMRR57 pKa = 11.84PLVKK61 pKa = 10.01LWRR64 pKa = 11.84KK65 pKa = 9.16IRR67 pKa = 11.84GPLMKK72 pKa = 10.1SGCTMM77 pKa = 4.51

Molecular weight: 9.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

229

0

229

50802

29

3105

221.8

24.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.992 ± 0.391

0.866 ± 0.095

6.85 ± 0.238

6.506 ± 0.381

4.025 ± 0.164

7.823 ± 0.326

1.655 ± 0.109

5.281 ± 0.152

5.445 ± 0.408

7.758 ± 0.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.163 ± 0.188

5.157 ± 0.223

3.911 ± 0.143

3.772 ± 0.134

4.352 ± 0.271

6.901 ± 0.266

7.317 ± 0.549

6.927 ± 0.185

1.309 ± 0.081

3.99 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski