Legionella lansingensis
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2630 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W0VQD9|A0A0W0VQD9_9GAMM Phosphopentomutase OS=Legionella lansingensis OX=45067 GN=deoB PE=3 SV=1
MM1 pKa = 7.41 AAINASPTSSGIYY14 pKa = 9.77 FSVSAADD21 pKa = 4.07 KK22 pKa = 9.87 VASLIAEE29 pKa = 4.14 EE30 pKa = 4.59 EE31 pKa = 4.21 NPNLNLRR38 pKa = 11.84 VFVTGGGCSGFQYY51 pKa = 11.02 GFTFDD56 pKa = 4.63 EE57 pKa = 5.59 KK58 pKa = 10.56 IQEE61 pKa = 4.56 DD62 pKa = 4.03 DD63 pKa = 3.66 TVIEE67 pKa = 4.33 QTCSNGQSSVKK78 pKa = 10.48 LLVDD82 pKa = 3.58 SMSYY86 pKa = 10.51 QYY88 pKa = 11.4 LNEE91 pKa = 4.08 AEE93 pKa = 3.91 IDD95 pKa = 4.01 YY96 pKa = 10.16 VQSIQGEE103 pKa = 4.29 QFVIRR108 pKa = 11.84 NPNAKK113 pKa = 8.39 STCGCGSSFSIEE125 pKa = 5.85 DD126 pKa = 3.46 EE127 pKa = 5.31 DD128 pKa = 4.75 EE129 pKa = 4.62 DD130 pKa = 4.13 NN131 pKa = 4.62
Molecular weight: 14.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.786
IPC2_protein 3.834
IPC_protein 3.757
Toseland 3.579
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.643
Rodwell 3.592
Grimsley 3.49
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 4.012
Thurlkill 3.617
EMBOSS 3.656
Sillero 3.872
Patrickios 0.54
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A0W0VKD9|A0A0W0VKD9_9GAMM Uncharacterized protein OS=Legionella lansingensis OX=45067 GN=Llan_1824 PE=4 SV=1
MM1 pKa = 8.1 DD2 pKa = 5.08 GAQGRR7 pKa = 11.84 SRR9 pKa = 11.84 TGTEE13 pKa = 3.89 FNPPRR18 pKa = 11.84 DD19 pKa = 4.2 FKK21 pKa = 11.53 SLASTCFATWAFYY34 pKa = 9.49 WRR36 pKa = 11.84 LRR38 pKa = 11.84 PEE40 pKa = 4.05 SNRR43 pKa = 11.84 RR44 pKa = 11.84 PRR46 pKa = 11.84 LCRR49 pKa = 11.84 PLHH52 pKa = 5.41 NHH54 pKa = 6.36 SATQPKK60 pKa = 10.27 LNPKK64 pKa = 9.62 KK65 pKa = 10.55 KK66 pKa = 9.68 SDD68 pKa = 3.67 YY69 pKa = 10.53 GVALIWSGKK78 pKa = 8.91 RR79 pKa = 11.84 DD80 pKa = 3.87 SNSRR84 pKa = 11.84 PQPWQGCALPTEE96 pKa = 5.06 LFPPLYY102 pKa = 10.47 GLPFYY107 pKa = 10.93 RR108 pKa = 11.84 KK109 pKa = 9.31 FLRR112 pKa = 11.84 CQQVNAKK119 pKa = 9.94 FIRR122 pKa = 11.84 NLNTSHH128 pKa = 7.59 KK129 pKa = 10.23 FYY131 pKa = 10.96 TFLNDD136 pKa = 3.05 SS137 pKa = 3.67
Molecular weight: 16.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.706
IPC_protein 10.321
Toseland 10.555
ProMoST 10.204
Dawson 10.687
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.994
Grimsley 10.73
Solomon 10.745
Lehninger 10.716
Nozaki 10.555
DTASelect 10.365
Thurlkill 10.57
EMBOSS 10.95
Sillero 10.613
Patrickios 10.716
IPC_peptide 10.76
IPC2_peptide 9.458
IPC2.peptide.svr19 8.504
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2630
0
2630
866165
50
2863
329.3
36.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.107 ± 0.049
1.181 ± 0.019
4.825 ± 0.037
6.06 ± 0.052
4.488 ± 0.037
6.137 ± 0.049
2.561 ± 0.023
7.22 ± 0.042
6.03 ± 0.044
11.185 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.343 ± 0.023
4.405 ± 0.038
4.19 ± 0.03
4.543 ± 0.039
4.592 ± 0.034
6.201 ± 0.033
5.285 ± 0.033
6.104 ± 0.042
1.181 ± 0.018
3.358 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here