Amino acid dipepetide frequency for Anaeromassilibacillus sp. An172

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.41AlaAla: 6.41 ± 0.122	1.016AlaCys: 1.016 ± 0.037	4.399AlaAsp: 4.399 ± 0.074	5.474AlaGlu: 5.474 ± 0.095	2.982AlaPhe: 2.982 ± 0.059	5.144AlaGly: 5.144 ± 0.093	0.922AlaHis: 0.922 ± 0.032	4.909AlaIle: 4.909 ± 0.091	4.753AlaLys: 4.753 ± 0.094	6.399AlaLeu: 6.399 ± 0.115	2.194AlaMet: 2.194 ± 0.059	2.771AlaAsn: 2.771 ± 0.067	1.923AlaPro: 1.923 ± 0.05	2.303AlaGln: 2.303 ± 0.057	2.357AlaArg: 2.357 ± 0.062	3.9AlaSer: 3.9 ± 0.079	3.32AlaThr: 3.32 ± 0.103	6.563AlaVal: 6.563 ± 0.095	0.472AlaTrp: 0.472 ± 0.024	2.527AlaTyr: 2.527 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	1.075CysAla: 1.075 ± 0.038	0.324CysCys: 0.324 ± 0.02	0.995CysAsp: 0.995 ± 0.033	1.075CysGlu: 1.075 ± 0.033	0.704CysPhe: 0.704 ± 0.028	1.743CysGly: 1.743 ± 0.051	0.284CysHis: 0.284 ± 0.02	1.064CysIle: 1.064 ± 0.044	0.956CysLys: 0.956 ± 0.044	1.127CysLeu: 1.127 ± 0.042	0.391CysMet: 0.391 ± 0.02	0.639CysAsn: 0.639 ± 0.031	0.712CysPro: 0.712 ± 0.033	0.38CysGln: 0.38 ± 0.022	0.711CysArg: 0.711 ± 0.028	1.115CysSer: 1.115 ± 0.036	0.799CysThr: 0.799 ± 0.033	1.102CysVal: 1.102 ± 0.038	0.107CysTrp: 0.107 ± 0.013	0.571CysTyr: 0.571 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	3.742AspAla: 3.742 ± 0.074	0.936AspCys: 0.936 ± 0.039	3.331AspAsp: 3.331 ± 0.084	4.854AspGlu: 4.854 ± 0.075	3.072AspPhe: 3.072 ± 0.077	4.943AspGly: 4.943 ± 0.123	0.732AspHis: 0.732 ± 0.03	4.931AspIle: 4.931 ± 0.078	4.223AspLys: 4.223 ± 0.072	4.278AspLeu: 4.278 ± 0.077	1.816AspMet: 1.816 ± 0.044	2.883AspAsn: 2.883 ± 0.076	1.747AspPro: 1.747 ± 0.053	1.006AspGln: 1.006 ± 0.036	2.158AspArg: 2.158 ± 0.052	3.466AspSer: 3.466 ± 0.064	3.313AspThr: 3.313 ± 0.067	3.921AspVal: 3.921 ± 0.08	0.536AspTrp: 0.536 ± 0.028	2.831AspTyr: 2.831 ± 0.066	0.0AspXaa: 0.0 ± 0.0
Glu	4.967GluAla: 4.967 ± 0.086	0.834GluCys: 0.834 ± 0.033	4.111GluAsp: 4.111 ± 0.08	6.562GluGlu: 6.562 ± 0.135	2.848GluPhe: 2.848 ± 0.062	4.036GluGly: 4.036 ± 0.091	1.013GluHis: 1.013 ± 0.034	6.388GluIle: 6.388 ± 0.096	7.093GluLys: 7.093 ± 0.125	6.271GluLeu: 6.271 ± 0.114	2.194GluMet: 2.194 ± 0.054	5.539GluAsn: 5.539 ± 0.091	2.029GluPro: 2.029 ± 0.056	2.535GluGln: 2.535 ± 0.062	2.937GluArg: 2.937 ± 0.072	3.644GluSer: 3.644 ± 0.068	4.143GluThr: 4.143 ± 0.09	4.059GluVal: 4.059 ± 0.074	0.503GluTrp: 0.503 ± 0.027	3.226GluTyr: 3.226 ± 0.076	0.0GluXaa: 0.0 ± 0.0
Phe	3.002PheAla: 3.002 ± 0.064	0.865PheCys: 0.865 ± 0.034	2.858PheAsp: 2.858 ± 0.064	3.069PheGlu: 3.069 ± 0.056	2.077PhePhe: 2.077 ± 0.062	3.042PheGly: 3.042 ± 0.074	0.647PheHis: 0.647 ± 0.029	3.278PheIle: 3.278 ± 0.076	2.605PheLys: 2.605 ± 0.064	3.713PheLeu: 3.713 ± 0.087	1.171PheMet: 1.171 ± 0.043	2.051PheAsn: 2.051 ± 0.05	1.358PhePro: 1.358 ± 0.047	1.059PheGln: 1.059 ± 0.039	1.501PheArg: 1.501 ± 0.044	3.567PheSer: 3.567 ± 0.086	2.655PheThr: 2.655 ± 0.079	2.861PheVal: 2.861 ± 0.061	0.39PheTrp: 0.39 ± 0.023	1.82PheTyr: 1.82 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	4.646GlyAla: 4.646 ± 0.098	1.24GlyCys: 1.24 ± 0.049	4.002GlyAsp: 4.002 ± 0.095	4.952GlyGlu: 4.952 ± 0.096	3.172GlyPhe: 3.172 ± 0.073	5.098GlyGly: 5.098 ± 0.125	1.01GlyHis: 1.01 ± 0.032	5.886GlyIle: 5.886 ± 0.106	5.769GlyLys: 5.769 ± 0.085	5.297GlyLeu: 5.297 ± 0.086	2.034GlyMet: 2.034 ± 0.052	3.547GlyAsn: 3.547 ± 0.089	0.97GlyPro: 0.97 ± 0.041	1.787GlyGln: 1.787 ± 0.049	2.747GlyArg: 2.747 ± 0.06	4.175GlySer: 4.175 ± 0.089	4.331GlyThr: 4.331 ± 0.1	5.134GlyVal: 5.134 ± 0.091	0.73GlyTrp: 0.73 ± 0.035	3.133GlyTyr: 3.133 ± 0.066	0.0GlyXaa: 0.0 ± 0.0
His	0.86HisAla: 0.86 ± 0.031	0.317HisCys: 0.317 ± 0.02	0.812HisAsp: 0.812 ± 0.033	0.859HisGlu: 0.859 ± 0.035	0.652HisPhe: 0.652 ± 0.027	1.145HisGly: 1.145 ± 0.038	0.343HisHis: 0.343 ± 0.022	1.23HisIle: 1.23 ± 0.041	0.958HisLys: 0.958 ± 0.037	1.253HisLeu: 1.253 ± 0.038	0.381HisMet: 0.381 ± 0.02	0.759HisAsn: 0.759 ± 0.029	0.725HisPro: 0.725 ± 0.035	0.4HisGln: 0.4 ± 0.023	0.722HisArg: 0.722 ± 0.029	0.941HisSer: 0.941 ± 0.037	0.832HisThr: 0.832 ± 0.032	0.786HisVal: 0.786 ± 0.027	0.141HisTrp: 0.141 ± 0.012	0.592HisTyr: 0.592 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.529IleAla: 5.529 ± 0.086	1.378IleCys: 1.378 ± 0.045	4.632IleAsp: 4.632 ± 0.086	5.326IleGlu: 5.326 ± 0.082	3.221IlePhe: 3.221 ± 0.081	4.591IleGly: 4.591 ± 0.098	1.125IleHis: 1.125 ± 0.039	5.712IleIle: 5.712 ± 0.117	5.224IleLys: 5.224 ± 0.083	6.367IleLeu: 6.367 ± 0.103	2.031IleMet: 2.031 ± 0.058	3.696IleAsn: 3.696 ± 0.077	3.044IlePro: 3.044 ± 0.057	1.886IleGln: 1.886 ± 0.049	2.938IleArg: 2.938 ± 0.075	5.657IleSer: 5.657 ± 0.091	4.958IleThr: 4.958 ± 0.103	5.064IleVal: 5.064 ± 0.086	0.551IleTrp: 0.551 ± 0.026	2.966IleTyr: 2.966 ± 0.06	0.0IleXaa: 0.0 ± 0.0
Lys	5.244LysAla: 5.244 ± 0.089	0.865LysCys: 0.865 ± 0.036	4.148LysAsp: 4.148 ± 0.079	6.198LysGlu: 6.198 ± 0.122	2.263LysPhe: 2.263 ± 0.055	4.428LysGly: 4.428 ± 0.086	0.976LysHis: 0.976 ± 0.032	5.865LysIle: 5.865 ± 0.097	6.401LysLys: 6.401 ± 0.127	5.948LysLeu: 5.948 ± 0.093	2.003LysMet: 2.003 ± 0.055	4.532LysAsn: 4.532 ± 0.086	2.191LysPro: 2.191 ± 0.057	1.996LysGln: 1.996 ± 0.057	3.188LysArg: 3.188 ± 0.065	4.253LysSer: 4.253 ± 0.081	4.343LysThr: 4.343 ± 0.065	4.237LysVal: 4.237 ± 0.073	0.598LysTrp: 0.598 ± 0.029	3.203LysTyr: 3.203 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	5.838LeuAla: 5.838 ± 0.107	1.614LeuCys: 1.614 ± 0.049	4.703LeuAsp: 4.703 ± 0.075	5.72LeuGlu: 5.72 ± 0.101	3.742LeuPhe: 3.742 ± 0.092	5.457LeuGly: 5.457 ± 0.089	1.184LeuHis: 1.184 ± 0.035	5.858LeuIle: 5.858 ± 0.103	6.428LeuLys: 6.428 ± 0.119	7.462LeuLeu: 7.462 ± 0.148	2.382LeuMet: 2.382 ± 0.059	4.181LeuAsn: 4.181 ± 0.077	3.256LeuPro: 3.256 ± 0.068	2.311LeuGln: 2.311 ± 0.057	3.441LeuArg: 3.441 ± 0.09	6.677LeuSer: 6.677 ± 0.118	4.891LeuThr: 4.891 ± 0.084	4.963LeuVal: 4.963 ± 0.083	0.701LeuTrp: 0.701 ± 0.03	3.328LeuTyr: 3.328 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	2.29MetAla: 2.29 ± 0.053	0.384MetCys: 0.384 ± 0.022	1.65MetAsp: 1.65 ± 0.046	2.061MetGlu: 2.061 ± 0.052	1.069MetPhe: 1.069 ± 0.036	2.051MetGly: 2.051 ± 0.05	0.362MetHis: 0.362 ± 0.02	1.828MetIle: 1.828 ± 0.049	2.298MetLys: 2.298 ± 0.061	2.436MetLeu: 2.436 ± 0.057	0.816MetMet: 0.816 ± 0.032	1.462MetAsn: 1.462 ± 0.043	1.102MetPro: 1.102 ± 0.042	0.794MetGln: 0.794 ± 0.037	1.133MetArg: 1.133 ± 0.035	1.835MetSer: 1.835 ± 0.047	1.58MetThr: 1.58 ± 0.04	1.649MetVal: 1.649 ± 0.041	0.203MetTrp: 0.203 ± 0.016	0.949MetTyr: 0.949 ± 0.034	0.0MetXaa: 0.0 ± 0.0
Asn	3.385AsnAla: 3.385 ± 0.087	0.844AsnCys: 0.844 ± 0.036	2.655AsnAsp: 2.655 ± 0.061	3.098AsnGlu: 3.098 ± 0.068	2.121AsnPhe: 2.121 ± 0.052	4.21AsnGly: 4.21 ± 0.114	0.838AsnHis: 0.838 ± 0.035	4.192AsnIle: 4.192 ± 0.076	3.315AsnLys: 3.315 ± 0.069	4.129AsnLeu: 4.129 ± 0.081	1.465AsnMet: 1.465 ± 0.051	2.761AsnAsn: 2.761 ± 0.082	2.306AsnPro: 2.306 ± 0.056	1.452AsnGln: 1.452 ± 0.048	1.958AsnArg: 1.958 ± 0.049	3.418AsnSer: 3.418 ± 0.08	2.911AsnThr: 2.911 ± 0.071	3.185AsnVal: 3.185 ± 0.08	0.439AsnTrp: 0.439 ± 0.026	2.184AsnTyr: 2.184 ± 0.059	0.0AsnXaa: 0.0 ± 0.0
Pro	2.195ProAla: 2.195 ± 0.065	0.471ProCys: 0.471 ± 0.027	2.274ProAsp: 2.274 ± 0.053	3.138ProGlu: 3.138 ± 0.081	1.606ProPhe: 1.606 ± 0.041	1.864ProGly: 1.864 ± 0.048	0.554ProHis: 0.554 ± 0.026	2.025ProIle: 2.025 ± 0.052	2.099ProLys: 2.099 ± 0.065	2.564ProLeu: 2.564 ± 0.064	0.875ProMet: 0.875 ± 0.031	1.42ProAsn: 1.42 ± 0.043	0.853ProPro: 0.853 ± 0.035	1.315ProGln: 1.315 ± 0.046	0.924ProArg: 0.924 ± 0.039	1.871ProSer: 1.871 ± 0.046	1.899ProThr: 1.899 ± 0.066	2.825ProVal: 2.825 ± 0.059	0.292ProTrp: 0.292 ± 0.02	1.394ProTyr: 1.394 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	1.978GlnAla: 1.978 ± 0.048	0.388GlnCys: 0.388 ± 0.024	1.239GlnAsp: 1.239 ± 0.043	2.201GlnGlu: 2.201 ± 0.061	1.057GlnPhe: 1.057 ± 0.035	1.895GlnGly: 1.895 ± 0.057	0.413GlnHis: 0.413 ± 0.023	2.221GlnIle: 2.221 ± 0.053	2.362GlnLys: 2.362 ± 0.066	2.434GlnLeu: 2.434 ± 0.058	0.906GlnMet: 0.906 ± 0.037	1.668GlnAsn: 1.668 ± 0.044	0.854GlnPro: 0.854 ± 0.036	1.111GlnGln: 1.111 ± 0.04	1.353GlnArg: 1.353 ± 0.04	1.774GlnSer: 1.774 ± 0.051	1.499GlnThr: 1.499 ± 0.044	1.654GlnVal: 1.654 ± 0.046	0.352GlnTrp: 0.352 ± 0.025	1.201GlnTyr: 1.201 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	2.318ArgAla: 2.318 ± 0.067	0.639ArgCys: 0.639 ± 0.033	2.106ArgAsp: 2.106 ± 0.054	3.28ArgGlu: 3.28 ± 0.086	1.873ArgPhe: 1.873 ± 0.047	2.165ArgGly: 2.165 ± 0.057	0.677ArgHis: 0.677 ± 0.031	3.106ArgIle: 3.106 ± 0.061	3.217ArgLys: 3.217 ± 0.062	3.503ArgLeu: 3.503 ± 0.081	1.13ArgMet: 1.13 ± 0.036	2.015ArgAsn: 2.015 ± 0.05	1.116ArgPro: 1.116 ± 0.042	1.275ArgGln: 1.275 ± 0.044	1.869ArgArg: 1.869 ± 0.057	2.07ArgSer: 2.07 ± 0.05	1.992ArgThr: 1.992 ± 0.055	2.468ArgVal: 2.468 ± 0.062	0.362ArgTrp: 0.362 ± 0.025	1.769ArgTyr: 1.769 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	4.651SerAla: 4.651 ± 0.091	0.897SerCys: 0.897 ± 0.041	3.947SerAsp: 3.947 ± 0.087	4.584SerGlu: 4.584 ± 0.089	3.154SerPhe: 3.154 ± 0.075	5.421SerGly: 5.421 ± 0.092	1.004SerHis: 1.004 ± 0.035	4.329SerIle: 4.329 ± 0.078	4.011SerLys: 4.011 ± 0.068	5.601SerLeu: 5.601 ± 0.082	1.641SerMet: 1.641 ± 0.049	2.709SerAsn: 2.709 ± 0.066	2.014SerPro: 2.014 ± 0.053	2.145SerGln: 2.145 ± 0.055	2.626SerArg: 2.626 ± 0.066	4.282SerSer: 4.282 ± 0.111	3.154SerThr: 3.154 ± 0.071	5.025SerVal: 5.025 ± 0.109	0.539SerTrp: 0.539 ± 0.03	2.526SerTyr: 2.526 ± 0.068	0.0SerXaa: 0.0 ± 0.0
Thr	5.099ThrAla: 5.099 ± 0.104	0.668ThrCys: 0.668 ± 0.028	3.654ThrAsp: 3.654 ± 0.081	4.484ThrGlu: 4.484 ± 0.108	2.359ThrPhe: 2.359 ± 0.063	4.629ThrGly: 4.629 ± 0.094	0.842ThrHis: 0.842 ± 0.034	4.118ThrIle: 4.118 ± 0.114	3.249ThrLys: 3.249 ± 0.062	4.847ThrLeu: 4.847 ± 0.075	1.411ThrMet: 1.411 ± 0.044	2.226ThrAsn: 2.226 ± 0.068	2.242ThrPro: 2.242 ± 0.051	1.566ThrGln: 1.566 ± 0.044	1.747ThrArg: 1.747 ± 0.053	3.238ThrSer: 3.238 ± 0.082	3.254ThrThr: 3.254 ± 0.095	5.234ThrVal: 5.234 ± 0.105	0.496ThrTrp: 0.496 ± 0.03	2.089ThrTyr: 2.089 ± 0.068	0.0ThrXaa: 0.0 ± 0.0
Val	4.415ValAla: 4.415 ± 0.097	1.253ValCys: 1.253 ± 0.046	3.905ValAsp: 3.905 ± 0.081	4.559ValGlu: 4.559 ± 0.064	3.357ValPhe: 3.357 ± 0.078	4.182ValGly: 4.182 ± 0.085	0.934ValHis: 0.934 ± 0.035	5.34ValIle: 5.34 ± 0.085	4.714ValLys: 4.714 ± 0.08	6.354ValLeu: 6.354 ± 0.102	1.822ValMet: 1.822 ± 0.05	3.272ValAsn: 3.272 ± 0.086	2.494ValPro: 2.494 ± 0.052	1.724ValGln: 1.724 ± 0.047	2.493ValArg: 2.493 ± 0.055	5.054ValSer: 5.054 ± 0.094	4.541ValThr: 4.541 ± 0.105	4.78ValVal: 4.78 ± 0.09	0.593ValTrp: 0.593 ± 0.026	2.798ValTyr: 2.798 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.535TrpAla: 0.535 ± 0.029	0.154TrpCys: 0.154 ± 0.017	0.543TrpAsp: 0.543 ± 0.03	0.629TrpGlu: 0.629 ± 0.026	0.388TrpPhe: 0.388 ± 0.025	0.577TrpGly: 0.577 ± 0.028	0.199TrpHis: 0.199 ± 0.018	0.597TrpIle: 0.597 ± 0.032	0.609TrpLys: 0.609 ± 0.033	0.707TrpLeu: 0.707 ± 0.031	0.235TrpMet: 0.235 ± 0.018	0.498TrpAsn: 0.498 ± 0.025	0.149TrpPro: 0.149 ± 0.014	0.352TrpGln: 0.352 ± 0.025	0.324TrpArg: 0.324 ± 0.022	0.463TrpSer: 0.463 ± 0.029	0.477TrpThr: 0.477 ± 0.025	0.469TrpVal: 0.469 ± 0.027	0.094TrpTrp: 0.094 ± 0.012	0.411TrpTyr: 0.411 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.56TyrAla: 2.56 ± 0.064	0.789TyrCys: 0.789 ± 0.03	2.757TyrAsp: 2.757 ± 0.065	2.81TyrGlu: 2.81 ± 0.067	1.874TyrPhe: 1.874 ± 0.049	3.039TyrGly: 3.039 ± 0.06	0.653TyrHis: 0.653 ± 0.029	3.022TyrIle: 3.022 ± 0.062	2.594TyrLys: 2.594 ± 0.058	3.446TyrLeu: 3.446 ± 0.08	1.051TyrMet: 1.051 ± 0.04	2.238TyrAsn: 2.238 ± 0.061	1.372TyrPro: 1.372 ± 0.047	1.164TyrGln: 1.164 ± 0.038	1.821TyrArg: 1.821 ± 0.045	2.857TyrSer: 2.857 ± 0.074	2.56TyrThr: 2.56 ± 0.072	2.601TyrVal: 2.601 ± 0.059	0.309TyrTrp: 0.309 ± 0.021	1.852TyrTyr: 1.852 ± 0.069	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2508 proteins (812747 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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