Lake Sarah-associated circular virus-27

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQS3|A0A140AQS3_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-27 OX=1685754 PE=3 SV=1
MM1 pKa = 8.02DD2 pKa = 4.38PQGRR6 pKa = 11.84GWCFTLNNPDD16 pKa = 4.55DD17 pKa = 5.46LDD19 pKa = 3.82VLLPQSWDD27 pKa = 3.38PDD29 pKa = 3.82SYY31 pKa = 11.91DD32 pKa = 3.35YY33 pKa = 11.38LVYY36 pKa = 10.2HH37 pKa = 6.84LEE39 pKa = 4.02MGAEE43 pKa = 4.64GTAHH47 pKa = 6.03LQGYY51 pKa = 9.21LYY53 pKa = 10.24FGSRR57 pKa = 11.84IRR59 pKa = 11.84FSQLKK64 pKa = 9.2KK65 pKa = 8.57WFPGSRR71 pKa = 11.84VHH73 pKa = 7.86LEE75 pKa = 3.86AAKK78 pKa = 9.08GTAVQNQRR86 pKa = 11.84YY87 pKa = 6.1CTKK90 pKa = 10.69EE91 pKa = 3.67EE92 pKa = 4.14GRR94 pKa = 11.84LEE96 pKa = 4.31GPWEE100 pKa = 4.42FGSLPDD106 pKa = 3.7QSQGKK111 pKa = 10.08RR112 pKa = 11.84NDD114 pKa = 3.6LLAVKK119 pKa = 10.44ADD121 pKa = 3.99LDD123 pKa = 4.08AGASLLEE130 pKa = 4.18IADD133 pKa = 3.81DD134 pKa = 3.79HH135 pKa = 6.6FSAFVRR141 pKa = 11.84YY142 pKa = 8.79HH143 pKa = 7.0RR144 pKa = 11.84SFKK147 pKa = 10.4EE148 pKa = 3.83YY149 pKa = 10.55KK150 pKa = 9.86LLKK153 pKa = 10.72APVANVAKK161 pKa = 10.36KK162 pKa = 10.34IIVVWGPTGTGKK174 pKa = 8.17TRR176 pKa = 11.84WAFDD180 pKa = 3.64NYY182 pKa = 10.29PSAFWKK188 pKa = 9.1TKK190 pKa = 10.56SGGSQQFWDD199 pKa = 4.88GYY201 pKa = 10.49DD202 pKa = 3.6GEE204 pKa = 4.7SVIIVDD210 pKa = 5.04EE211 pKa = 4.88FYY213 pKa = 11.1GWLPFDD219 pKa = 3.9FMLRR223 pKa = 11.84FLDD226 pKa = 4.29RR227 pKa = 11.84YY228 pKa = 8.88PVKK231 pKa = 10.93LEE233 pKa = 4.05IKK235 pKa = 9.94HH236 pKa = 5.29GTVPLCADD244 pKa = 4.32TIIFTSNKK252 pKa = 9.39HH253 pKa = 4.83PSEE256 pKa = 4.11WYY258 pKa = 9.97HH259 pKa = 4.6IQRR262 pKa = 11.84YY263 pKa = 8.47KK264 pKa = 10.21WDD266 pKa = 3.41EE267 pKa = 4.21SNPLRR272 pKa = 11.84RR273 pKa = 11.84RR274 pKa = 11.84LTSVRR279 pKa = 11.84EE280 pKa = 3.79LRR282 pKa = 11.84ADD284 pKa = 3.31GFGPDD289 pKa = 3.42EE290 pKa = 4.52SSPGFSSSVAVVAPVFTTTTTTTTTSAGGLDD321 pKa = 3.57DD322 pKa = 4.02VPRR325 pKa = 11.84FRR327 pKa = 11.84NGYY330 pKa = 6.44PAPTVTDD337 pKa = 4.56LYY339 pKa = 11.47KK340 pKa = 10.94DD341 pKa = 3.47LL342 pKa = 5.36

Molecular weight: 38.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQS3|A0A140AQS3_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-27 OX=1685754 PE=3 SV=1
MM1 pKa = 7.45PRR3 pKa = 11.84GSIFAKK9 pKa = 10.38KK10 pKa = 10.22SSTYY14 pKa = 9.25QKK16 pKa = 10.65RR17 pKa = 11.84KK18 pKa = 9.11LAAAAKK24 pKa = 9.57AISAARR30 pKa = 11.84GTTLSSRR37 pKa = 11.84RR38 pKa = 11.84GLSAPLRR45 pKa = 11.84TGGFWGQEE53 pKa = 3.17RR54 pKa = 11.84RR55 pKa = 11.84MYY57 pKa = 10.91SGMGPEE63 pKa = 4.78LKK65 pKa = 10.65VIDD68 pKa = 4.49SGPVGPLGFATAGAVALLNGVAQGTDD94 pKa = 3.04DD95 pKa = 3.48TQRR98 pKa = 11.84IGRR101 pKa = 11.84RR102 pKa = 11.84ATIKK106 pKa = 10.65SILFRR111 pKa = 11.84AFINPTTTTDD121 pKa = 4.03NIGDD125 pKa = 3.45QCRR128 pKa = 11.84FLLVWDD134 pKa = 3.93NQANGTALTVANVLNSATFSEE155 pKa = 4.84PNNLDD160 pKa = 2.89NRR162 pKa = 11.84DD163 pKa = 3.45RR164 pKa = 11.84FKK166 pKa = 10.88IIWDD170 pKa = 3.12KK171 pKa = 10.39WITMNPAVYY180 pKa = 9.75AANVFTGGNPQCKK193 pKa = 8.61MVHH196 pKa = 6.38CYY198 pKa = 10.37KK199 pKa = 10.66KK200 pKa = 10.97LNLEE204 pKa = 4.14TTFNGSGATIGSIATGSLYY223 pKa = 10.93VIAIAQNATNNNYY236 pKa = 9.75VYY238 pKa = 10.3NCRR241 pKa = 11.84VRR243 pKa = 11.84FTDD246 pKa = 3.09AA247 pKa = 3.91

Molecular weight: 26.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

589

247

342

294.5

32.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.659 ± 1.717

1.188 ± 0.24

6.282 ± 1.244

3.396 ± 0.989

5.263 ± 0.451

8.659 ± 0.364

1.528 ± 0.626

4.244 ± 1.019

5.263 ± 0.226

7.98 ± 0.611

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.358 ± 0.371

4.924 ± 1.768

5.093 ± 0.808

3.226 ± 0.007

6.282 ± 0.335

6.621 ± 0.306

7.81 ± 0.837

5.772 ± 0.284

2.547 ± 0.517

3.905 ± 0.597
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski