Beihai narna-like virus 1
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KI53|A0A1L3KI53_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 1 OX=1922436 PE=4 SV=1
MM1 pKa = 7.86 DD2 pKa = 6.2 PIEE5 pKa = 4.43 VSHH8 pKa = 6.95 ALYY11 pKa = 10.52 EE12 pKa = 4.88 GLASWCMSRR21 pKa = 11.84 RR22 pKa = 11.84 KK23 pKa = 8.47 GTKK26 pKa = 9.76 DD27 pKa = 3.18 PNFQDD32 pKa = 3.53 LCRR35 pKa = 11.84 LARR38 pKa = 11.84 HH39 pKa = 6.51 CGLCEE44 pKa = 3.79 PPKK47 pKa = 10.8 CLDD50 pKa = 3.34 SALTEE55 pKa = 4.28 FLNSFDD61 pKa = 4.25 PPSCVEE67 pKa = 4.01 HH68 pKa = 7.69 DD69 pKa = 4.41 NILKK73 pKa = 10.02 EE74 pKa = 4.39 RR75 pKa = 11.84 IVHH78 pKa = 7.08 SIQQANRR85 pKa = 11.84 MVAVYY90 pKa = 10.27 TDD92 pKa = 3.03 ILKK95 pKa = 10.61 FYY97 pKa = 10.36 GVLGRR102 pKa = 11.84 LHH104 pKa = 6.55 PKK106 pKa = 8.68 GWLWYY111 pKa = 9.74 GVLGLLEE118 pKa = 5.75 KK119 pKa = 10.25 YY120 pKa = 10.32 LKK122 pKa = 10.25 WSTADD127 pKa = 3.45 FLARR131 pKa = 11.84 TLDD134 pKa = 4.33 QEE136 pKa = 4.91 DD137 pKa = 4.7 LPPNPGFEE145 pKa = 4.47 LGLPFTGPAASHH157 pKa = 6.83 LKK159 pKa = 9.88 RR160 pKa = 11.84 VCLFNRR166 pKa = 11.84 KK167 pKa = 9.18 RR168 pKa = 11.84 GLRR171 pKa = 11.84 STYY174 pKa = 10.76 AVALAYY180 pKa = 10.35 SLYY183 pKa = 9.5 QGKK186 pKa = 10.16 GGSLPVSAEE195 pKa = 3.7 FLEE198 pKa = 4.54 NSVEE202 pKa = 4.06 RR203 pKa = 11.84 AVNALCTPRR212 pKa = 11.84 TGMTEE217 pKa = 4.4 DD218 pKa = 3.33 IFDD221 pKa = 3.77 VMSEE225 pKa = 3.96 QVRR228 pKa = 11.84 RR229 pKa = 11.84 TVRR232 pKa = 11.84 EE233 pKa = 3.91 CFPRR237 pKa = 11.84 HH238 pKa = 4.97 HH239 pKa = 6.9 SEE241 pKa = 3.69 RR242 pKa = 11.84 ARR244 pKa = 11.84 PGSHH248 pKa = 7.41 RR249 pKa = 11.84 IPSLRR254 pKa = 11.84 SSFQNSRR261 pKa = 11.84 KK262 pKa = 9.44 DD263 pKa = 3.04 WGAFGHH269 pKa = 6.34 IVSASISIGFPYY281 pKa = 10.93 LLGHH285 pKa = 6.18 WHH287 pKa = 6.89 HH288 pKa = 6.46 KK289 pKa = 8.86 TNIEE293 pKa = 3.99 LVYY296 pKa = 10.78 GPSDD300 pKa = 3.81 PEE302 pKa = 4.38 DD303 pKa = 3.29 VQLDD307 pKa = 3.84 YY308 pKa = 10.98 EE309 pKa = 4.2 QSYY312 pKa = 10.19 RR313 pKa = 11.84 RR314 pKa = 11.84 AVLMGRR320 pKa = 11.84 CRR322 pKa = 11.84 CYY324 pKa = 10.21 PVGLLEE330 pKa = 4.16 PFKK333 pKa = 11.21 VRR335 pKa = 11.84 VITRR339 pKa = 11.84 GDD341 pKa = 3.03 ADD343 pKa = 3.76 MYY345 pKa = 10.73 HH346 pKa = 7.53 LARR349 pKa = 11.84 RR350 pKa = 11.84 WQKK353 pKa = 9.5 TIHH356 pKa = 5.9 GCLRR360 pKa = 11.84 QHH362 pKa = 6.69 PTFQLIGGPIDD373 pKa = 3.65 WSALNHH379 pKa = 5.75 LSEE382 pKa = 4.62 RR383 pKa = 11.84 TDD385 pKa = 3.13 ICDD388 pKa = 3.21 DD389 pKa = 4.26 RR390 pKa = 11.84 YY391 pKa = 10.03 WVSADD396 pKa = 3.41 YY397 pKa = 10.41 TSATDD402 pKa = 3.75 NLDD405 pKa = 3.75 PEE407 pKa = 4.66 LSRR410 pKa = 11.84 VCMEE414 pKa = 5.24 EE415 pKa = 3.11 ICQRR419 pKa = 11.84 LGVPLEE425 pKa = 4.37 DD426 pKa = 3.34 QLVLLRR432 pKa = 11.84 TLTDD436 pKa = 3.13 HH437 pKa = 7.68 DD438 pKa = 5.11 LYY440 pKa = 11.24 EE441 pKa = 4.39 EE442 pKa = 4.31 SGDD445 pKa = 4.88 LIGSQAWGQLMGSPSSFPILCLINAAATRR474 pKa = 11.84 FALEE478 pKa = 3.7 LANWKK483 pKa = 9.96 VGEE486 pKa = 4.27 YY487 pKa = 10.79 KK488 pKa = 10.18 LTDD491 pKa = 3.59 LPMLFNGDD499 pKa = 3.55 DD500 pKa = 3.32 AAFRR504 pKa = 11.84 ANEE507 pKa = 3.8 QEE509 pKa = 3.88 YY510 pKa = 8.91 TIWKK514 pKa = 9.71 GITKK518 pKa = 10.2 SVGLEE523 pKa = 3.54 FSVGKK528 pKa = 10.31 NFFHH532 pKa = 7.47 KK533 pKa = 10.45 RR534 pKa = 11.84 FLILNSAVHH543 pKa = 6.39 EE544 pKa = 4.28 DD545 pKa = 3.34 VGRR548 pKa = 11.84 VDD550 pKa = 3.62 FFGRR554 pKa = 11.84 RR555 pKa = 11.84 FSRR558 pKa = 11.84 LEE560 pKa = 3.7 PVVTLSSSLLYY571 pKa = 10.65 GQVRR575 pKa = 11.84 VQSASQNDD583 pKa = 3.92 RR584 pKa = 11.84 THH586 pKa = 7.16 LCQSDD591 pKa = 3.88 LSVGRR596 pKa = 11.84 SLGKK600 pKa = 9.08 MCEE603 pKa = 4.12 DD604 pKa = 3.88 LVAGHH609 pKa = 6.07 SEE611 pKa = 3.97 EE612 pKa = 5.07 QRR614 pKa = 11.84 DD615 pKa = 3.77 FLIKK619 pKa = 10.52 RR620 pKa = 11.84 FIQVNKK626 pKa = 9.27 TLLAKK631 pKa = 10.31 CPKK634 pKa = 8.8 GQAWCIPRR642 pKa = 11.84 RR643 pKa = 11.84 LGGLGLPAPSGYY655 pKa = 9.96 EE656 pKa = 3.67 PRR658 pKa = 11.84 PPQAKK663 pKa = 9.43 LAAYY667 pKa = 9.08 LATRR671 pKa = 11.84 QLDD674 pKa = 3.84 DD675 pKa = 4.71 PDD677 pKa = 4.12 LRR679 pKa = 11.84 DD680 pKa = 3.59 YY681 pKa = 11.12 LYY683 pKa = 10.23 PDD685 pKa = 3.09 QPDD688 pKa = 3.73 FLRR691 pKa = 11.84 AYY693 pKa = 9.63 ISYY696 pKa = 10.33 RR697 pKa = 11.84 QDD699 pKa = 2.72 LCRR702 pKa = 11.84 KK703 pKa = 9.98 LGIEE707 pKa = 4.13 STNEE711 pKa = 3.57 QVVTLEE717 pKa = 4.84 LPDD720 pKa = 3.79 LALWAPMGAAGGDD733 pKa = 3.21 KK734 pKa = 10.59 TEE736 pKa = 4.03 LVKK739 pKa = 10.89 RR740 pKa = 11.84 SIRR743 pKa = 11.84 AWEE746 pKa = 4.02 SLKK749 pKa = 10.83 KK750 pKa = 10.36 RR751 pKa = 11.84 GLATSLHH758 pKa = 5.44 AMKK761 pKa = 9.8 MDD763 pKa = 5.04 TIRR766 pKa = 11.84 HH767 pKa = 4.41 WTQEE771 pKa = 3.75 PRR773 pKa = 11.84 NIADD777 pKa = 3.36 VSSWTVSWW785 pKa = 3.5
Molecular weight: 89.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.746
IPC2_protein 6.927
IPC_protein 6.839
Toseland 6.737
ProMoST 7.38
Dawson 7.454
Bjellqvist 7.775
Wikipedia 7.38
Rodwell 7.468
Grimsley 6.825
Solomon 7.468
Lehninger 7.497
Nozaki 8.068
DTASelect 7.702
Thurlkill 7.746
EMBOSS 7.732
Sillero 7.995
Patrickios 4.24
IPC_peptide 7.468
IPC2_peptide 7.322
IPC2.peptide.svr19 7.027
Protein with the highest isoelectric point:
>tr|A0A1L3KI53|A0A1L3KI53_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 1 OX=1922436 PE=4 SV=1
MM1 pKa = 7.86 DD2 pKa = 6.2 PIEE5 pKa = 4.43 VSHH8 pKa = 6.95 ALYY11 pKa = 10.52 EE12 pKa = 4.88 GLASWCMSRR21 pKa = 11.84 RR22 pKa = 11.84 KK23 pKa = 8.47 GTKK26 pKa = 9.76 DD27 pKa = 3.18 PNFQDD32 pKa = 3.53 LCRR35 pKa = 11.84 LARR38 pKa = 11.84 HH39 pKa = 6.51 CGLCEE44 pKa = 3.79 PPKK47 pKa = 10.8 CLDD50 pKa = 3.34 SALTEE55 pKa = 4.28 FLNSFDD61 pKa = 4.25 PPSCVEE67 pKa = 4.01 HH68 pKa = 7.69 DD69 pKa = 4.41 NILKK73 pKa = 10.02 EE74 pKa = 4.39 RR75 pKa = 11.84 IVHH78 pKa = 7.08 SIQQANRR85 pKa = 11.84 MVAVYY90 pKa = 10.27 TDD92 pKa = 3.03 ILKK95 pKa = 10.61 FYY97 pKa = 10.36 GVLGRR102 pKa = 11.84 LHH104 pKa = 6.55 PKK106 pKa = 8.68 GWLWYY111 pKa = 9.74 GVLGLLEE118 pKa = 5.75 KK119 pKa = 10.25 YY120 pKa = 10.32 LKK122 pKa = 10.25 WSTADD127 pKa = 3.45 FLARR131 pKa = 11.84 TLDD134 pKa = 4.33 QEE136 pKa = 4.91 DD137 pKa = 4.7 LPPNPGFEE145 pKa = 4.47 LGLPFTGPAASHH157 pKa = 6.83 LKK159 pKa = 9.88 RR160 pKa = 11.84 VCLFNRR166 pKa = 11.84 KK167 pKa = 9.18 RR168 pKa = 11.84 GLRR171 pKa = 11.84 STYY174 pKa = 10.76 AVALAYY180 pKa = 10.35 SLYY183 pKa = 9.5 QGKK186 pKa = 10.16 GGSLPVSAEE195 pKa = 3.7 FLEE198 pKa = 4.54 NSVEE202 pKa = 4.06 RR203 pKa = 11.84 AVNALCTPRR212 pKa = 11.84 TGMTEE217 pKa = 4.4 DD218 pKa = 3.33 IFDD221 pKa = 3.77 VMSEE225 pKa = 3.96 QVRR228 pKa = 11.84 RR229 pKa = 11.84 TVRR232 pKa = 11.84 EE233 pKa = 3.91 CFPRR237 pKa = 11.84 HH238 pKa = 4.97 HH239 pKa = 6.9 SEE241 pKa = 3.69 RR242 pKa = 11.84 ARR244 pKa = 11.84 PGSHH248 pKa = 7.41 RR249 pKa = 11.84 IPSLRR254 pKa = 11.84 SSFQNSRR261 pKa = 11.84 KK262 pKa = 9.44 DD263 pKa = 3.04 WGAFGHH269 pKa = 6.34 IVSASISIGFPYY281 pKa = 10.93 LLGHH285 pKa = 6.18 WHH287 pKa = 6.89 HH288 pKa = 6.46 KK289 pKa = 8.86 TNIEE293 pKa = 3.99 LVYY296 pKa = 10.78 GPSDD300 pKa = 3.81 PEE302 pKa = 4.38 DD303 pKa = 3.29 VQLDD307 pKa = 3.84 YY308 pKa = 10.98 EE309 pKa = 4.2 QSYY312 pKa = 10.19 RR313 pKa = 11.84 RR314 pKa = 11.84 AVLMGRR320 pKa = 11.84 CRR322 pKa = 11.84 CYY324 pKa = 10.21 PVGLLEE330 pKa = 4.16 PFKK333 pKa = 11.21 VRR335 pKa = 11.84 VITRR339 pKa = 11.84 GDD341 pKa = 3.03 ADD343 pKa = 3.76 MYY345 pKa = 10.73 HH346 pKa = 7.53 LARR349 pKa = 11.84 RR350 pKa = 11.84 WQKK353 pKa = 9.5 TIHH356 pKa = 5.9 GCLRR360 pKa = 11.84 QHH362 pKa = 6.69 PTFQLIGGPIDD373 pKa = 3.65 WSALNHH379 pKa = 5.75 LSEE382 pKa = 4.62 RR383 pKa = 11.84 TDD385 pKa = 3.13 ICDD388 pKa = 3.21 DD389 pKa = 4.26 RR390 pKa = 11.84 YY391 pKa = 10.03 WVSADD396 pKa = 3.41 YY397 pKa = 10.41 TSATDD402 pKa = 3.75 NLDD405 pKa = 3.75 PEE407 pKa = 4.66 LSRR410 pKa = 11.84 VCMEE414 pKa = 5.24 EE415 pKa = 3.11 ICQRR419 pKa = 11.84 LGVPLEE425 pKa = 4.37 DD426 pKa = 3.34 QLVLLRR432 pKa = 11.84 TLTDD436 pKa = 3.13 HH437 pKa = 7.68 DD438 pKa = 5.11 LYY440 pKa = 11.24 EE441 pKa = 4.39 EE442 pKa = 4.31 SGDD445 pKa = 4.88 LIGSQAWGQLMGSPSSFPILCLINAAATRR474 pKa = 11.84 FALEE478 pKa = 3.7 LANWKK483 pKa = 9.96 VGEE486 pKa = 4.27 YY487 pKa = 10.79 KK488 pKa = 10.18 LTDD491 pKa = 3.59 LPMLFNGDD499 pKa = 3.55 DD500 pKa = 3.32 AAFRR504 pKa = 11.84 ANEE507 pKa = 3.8 QEE509 pKa = 3.88 YY510 pKa = 8.91 TIWKK514 pKa = 9.71 GITKK518 pKa = 10.2 SVGLEE523 pKa = 3.54 FSVGKK528 pKa = 10.31 NFFHH532 pKa = 7.47 KK533 pKa = 10.45 RR534 pKa = 11.84 FLILNSAVHH543 pKa = 6.39 EE544 pKa = 4.28 DD545 pKa = 3.34 VGRR548 pKa = 11.84 VDD550 pKa = 3.62 FFGRR554 pKa = 11.84 RR555 pKa = 11.84 FSRR558 pKa = 11.84 LEE560 pKa = 3.7 PVVTLSSSLLYY571 pKa = 10.65 GQVRR575 pKa = 11.84 VQSASQNDD583 pKa = 3.92 RR584 pKa = 11.84 THH586 pKa = 7.16 LCQSDD591 pKa = 3.88 LSVGRR596 pKa = 11.84 SLGKK600 pKa = 9.08 MCEE603 pKa = 4.12 DD604 pKa = 3.88 LVAGHH609 pKa = 6.07 SEE611 pKa = 3.97 EE612 pKa = 5.07 QRR614 pKa = 11.84 DD615 pKa = 3.77 FLIKK619 pKa = 10.52 RR620 pKa = 11.84 FIQVNKK626 pKa = 9.27 TLLAKK631 pKa = 10.31 CPKK634 pKa = 8.8 GQAWCIPRR642 pKa = 11.84 RR643 pKa = 11.84 LGGLGLPAPSGYY655 pKa = 9.96 EE656 pKa = 3.67 PRR658 pKa = 11.84 PPQAKK663 pKa = 9.43 LAAYY667 pKa = 9.08 LATRR671 pKa = 11.84 QLDD674 pKa = 3.84 DD675 pKa = 4.71 PDD677 pKa = 4.12 LRR679 pKa = 11.84 DD680 pKa = 3.59 YY681 pKa = 11.12 LYY683 pKa = 10.23 PDD685 pKa = 3.09 QPDD688 pKa = 3.73 FLRR691 pKa = 11.84 AYY693 pKa = 9.63 ISYY696 pKa = 10.33 RR697 pKa = 11.84 QDD699 pKa = 2.72 LCRR702 pKa = 11.84 KK703 pKa = 9.98 LGIEE707 pKa = 4.13 STNEE711 pKa = 3.57 QVVTLEE717 pKa = 4.84 LPDD720 pKa = 3.79 LALWAPMGAAGGDD733 pKa = 3.21 KK734 pKa = 10.59 TEE736 pKa = 4.03 LVKK739 pKa = 10.89 RR740 pKa = 11.84 SIRR743 pKa = 11.84 AWEE746 pKa = 4.02 SLKK749 pKa = 10.83 KK750 pKa = 10.36 RR751 pKa = 11.84 GLATSLHH758 pKa = 5.44 AMKK761 pKa = 9.8 MDD763 pKa = 5.04 TIRR766 pKa = 11.84 HH767 pKa = 4.41 WTQEE771 pKa = 3.75 PRR773 pKa = 11.84 NIADD777 pKa = 3.36 VSSWTVSWW785 pKa = 3.5
Molecular weight: 89.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.746
IPC2_protein 6.927
IPC_protein 6.839
Toseland 6.737
ProMoST 7.38
Dawson 7.454
Bjellqvist 7.775
Wikipedia 7.38
Rodwell 7.468
Grimsley 6.825
Solomon 7.468
Lehninger 7.497
Nozaki 8.068
DTASelect 7.702
Thurlkill 7.746
EMBOSS 7.732
Sillero 7.995
Patrickios 4.24
IPC_peptide 7.468
IPC2_peptide 7.322
IPC2.peptide.svr19 7.027
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
785
785
785
785.0
89.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.752 ± 0.0
2.675 ± 0.0
6.242 ± 0.0
5.605 ± 0.0
3.822 ± 0.0
6.879 ± 0.0
3.057 ± 0.0
3.949 ± 0.0
4.204 ± 0.0
11.975 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.783 ± 0.0
2.803 ± 0.0
5.478 ± 0.0
3.822 ± 0.0
7.898 ± 0.0
7.389 ± 0.0
4.459 ± 0.0
5.605 ± 0.0
2.293 ± 0.0
3.312 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here