Amino acid dipepetide frequency for Oceanispirochaeta sp. M1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.344AlaAla: 6.344 ± 0.073	0.685AlaCys: 0.685 ± 0.021	3.866AlaAsp: 3.866 ± 0.05	4.889AlaGlu: 4.889 ± 0.049	2.92AlaPhe: 2.92 ± 0.043	5.74AlaGly: 5.74 ± 0.063	1.092AlaHis: 1.092 ± 0.025	4.074AlaIle: 4.074 ± 0.054	2.761AlaLys: 2.761 ± 0.045	7.699AlaLeu: 7.699 ± 0.081	1.925AlaMet: 1.925 ± 0.038	1.808AlaAsn: 1.808 ± 0.035	2.08AlaPro: 2.08 ± 0.033	1.804AlaGln: 1.804 ± 0.036	2.931AlaArg: 2.931 ± 0.05	4.421AlaSer: 4.421 ± 0.054	2.442AlaThr: 2.442 ± 0.043	5.513AlaVal: 5.513 ± 0.06	0.729AlaTrp: 0.729 ± 0.018	2.112AlaTyr: 2.112 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	0.572CysAla: 0.572 ± 0.019	0.159CysCys: 0.159 ± 0.011	0.584CysAsp: 0.584 ± 0.019	0.564CysGlu: 0.564 ± 0.016	0.477CysPhe: 0.477 ± 0.015	0.971CysGly: 0.971 ± 0.029	0.247CysHis: 0.247 ± 0.012	0.695CysIle: 0.695 ± 0.021	0.439CysLys: 0.439 ± 0.016	0.985CysLeu: 0.985 ± 0.028	0.241CysMet: 0.241 ± 0.012	0.377CysAsn: 0.377 ± 0.015	0.527CysPro: 0.527 ± 0.023	0.246CysGln: 0.246 ± 0.012	0.684CysArg: 0.684 ± 0.022	0.894CysSer: 0.894 ± 0.025	0.472CysThr: 0.472 ± 0.017	0.49CysVal: 0.49 ± 0.016	0.117CysTrp: 0.117 ± 0.009	0.37CysTyr: 0.37 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.433AspAla: 3.433 ± 0.054	0.583AspCys: 0.583 ± 0.019	3.264AspAsp: 3.264 ± 0.055	4.305AspGlu: 4.305 ± 0.053	3.216AspPhe: 3.216 ± 0.044	3.734AspGly: 3.734 ± 0.054	1.087AspHis: 1.087 ± 0.025	4.59AspIle: 4.59 ± 0.05	3.411AspLys: 3.411 ± 0.042	6.185AspLeu: 6.185 ± 0.065	1.71AspMet: 1.71 ± 0.032	2.387AspAsn: 2.387 ± 0.041	2.417AspPro: 2.417 ± 0.041	1.74AspGln: 1.74 ± 0.032	2.865AspArg: 2.865 ± 0.042	4.287AspSer: 4.287 ± 0.053	2.531AspThr: 2.531 ± 0.036	3.097AspVal: 3.097 ± 0.042	0.812AspTrp: 0.812 ± 0.025	2.462AspTyr: 2.462 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	4.895GluAla: 4.895 ± 0.058	0.523GluCys: 0.523 ± 0.019	4.26GluAsp: 4.26 ± 0.057	6.178GluGlu: 6.178 ± 0.066	2.56GluPhe: 2.56 ± 0.036	4.694GluGly: 4.694 ± 0.057	1.11GluHis: 1.11 ± 0.028	5.894GluIle: 5.894 ± 0.067	5.695GluLys: 5.695 ± 0.063	7.099GluLeu: 7.099 ± 0.078	2.185GluMet: 2.185 ± 0.035	3.955GluAsn: 3.955 ± 0.045	1.74GluPro: 1.74 ± 0.034	2.374GluGln: 2.374 ± 0.038	2.917GluArg: 2.917 ± 0.043	4.883GluSer: 4.883 ± 0.052	3.593GluThr: 3.593 ± 0.047	3.743GluVal: 3.743 ± 0.049	0.728GluTrp: 0.728 ± 0.023	2.607GluTyr: 2.607 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	2.66PheAla: 2.66 ± 0.044	0.552PheCys: 0.552 ± 0.018	2.506PheAsp: 2.506 ± 0.036	2.618PheGlu: 2.618 ± 0.037	2.587PhePhe: 2.587 ± 0.044	2.939PheGly: 2.939 ± 0.053	0.953PheHis: 0.953 ± 0.022	3.791PheIle: 3.791 ± 0.051	2.467PheLys: 2.467 ± 0.037	5.15PheLeu: 5.15 ± 0.056	1.261PheMet: 1.261 ± 0.027	2.105PheAsn: 2.105 ± 0.039	1.983PhePro: 1.983 ± 0.034	1.485PheGln: 1.485 ± 0.028	2.226PheArg: 2.226 ± 0.038	4.262PheSer: 4.262 ± 0.055	2.663PheThr: 2.663 ± 0.043	2.298PheVal: 2.298 ± 0.038	0.543PheTrp: 0.543 ± 0.019	1.827PheTyr: 1.827 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	4.599GlyAla: 4.599 ± 0.067	0.876GlyCys: 0.876 ± 0.027	3.676GlyAsp: 3.676 ± 0.053	4.476GlyGlu: 4.476 ± 0.058	3.675GlyPhe: 3.675 ± 0.053	4.98GlyGly: 4.98 ± 0.076	1.271GlyHis: 1.271 ± 0.023	6.28GlyIle: 6.28 ± 0.072	4.348GlyLys: 4.348 ± 0.044	6.832GlyLeu: 6.832 ± 0.066	2.298GlyMet: 2.298 ± 0.038	2.986GlyAsn: 2.986 ± 0.04	1.791GlyPro: 1.791 ± 0.033	1.986GlyGln: 1.986 ± 0.036	3.296GlyArg: 3.296 ± 0.047	4.814GlySer: 4.814 ± 0.055	3.647GlyThr: 3.647 ± 0.052	4.32GlyVal: 4.32 ± 0.05	0.867GlyTrp: 0.867 ± 0.025	2.763GlyTyr: 2.763 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	0.894HisAla: 0.894 ± 0.022	0.247HisCys: 0.247 ± 0.012	0.955HisAsp: 0.955 ± 0.021	1.052HisGlu: 1.052 ± 0.023	1.041HisPhe: 1.041 ± 0.025	1.169HisGly: 1.169 ± 0.028	0.465HisHis: 0.465 ± 0.016	1.404HisIle: 1.404 ± 0.029	1.068HisLys: 1.068 ± 0.023	1.824HisLeu: 1.824 ± 0.036	0.481HisMet: 0.481 ± 0.016	0.764HisAsn: 0.764 ± 0.018	0.973HisPro: 0.973 ± 0.024	0.548HisGln: 0.548 ± 0.015	0.904HisArg: 0.904 ± 0.024	1.379HisSer: 1.379 ± 0.03	0.819HisThr: 0.819 ± 0.019	0.833HisVal: 0.833 ± 0.023	0.247HisTrp: 0.247 ± 0.012	0.755HisTyr: 0.755 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	4.96IleAla: 4.96 ± 0.058	0.878IleCys: 0.878 ± 0.022	4.322IleAsp: 4.322 ± 0.052	4.956IleGlu: 4.956 ± 0.052	3.597IlePhe: 3.597 ± 0.053	4.571IleGly: 4.571 ± 0.055	1.56IleHis: 1.56 ± 0.032	6.068IleIle: 6.068 ± 0.064	4.391IleLys: 4.391 ± 0.057	8.856IleLeu: 8.856 ± 0.088	1.973IleMet: 1.973 ± 0.034	3.482IleAsn: 3.482 ± 0.045	4.095IlePro: 4.095 ± 0.053	2.503IleGln: 2.503 ± 0.04	4.024IleArg: 4.024 ± 0.047	6.443IleSer: 6.443 ± 0.068	3.957IleThr: 3.957 ± 0.052	4.107IleVal: 4.107 ± 0.055	0.719IleTrp: 0.719 ± 0.023	2.573IleTyr: 2.573 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	4.384LysAla: 4.384 ± 0.056	0.383LysCys: 0.383 ± 0.016	3.937LysAsp: 3.937 ± 0.044	5.454LysGlu: 5.454 ± 0.074	1.692LysPhe: 1.692 ± 0.032	4.37LysGly: 4.37 ± 0.054	0.93LysHis: 0.93 ± 0.025	4.814LysIle: 4.814 ± 0.056	5.135LysLys: 5.135 ± 0.058	4.961LysLeu: 4.961 ± 0.053	1.875LysMet: 1.875 ± 0.037	3.261LysAsn: 3.261 ± 0.048	1.876LysPro: 1.876 ± 0.031	1.831LysGln: 1.831 ± 0.032	2.793LysArg: 2.793 ± 0.041	4.106LysSer: 4.106 ± 0.05	3.4LysThr: 3.4 ± 0.041	3.437LysVal: 3.437 ± 0.052	0.624LysTrp: 0.624 ± 0.019	1.962LysTyr: 1.962 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	6.472LeuAla: 6.472 ± 0.078	1.065LeuCys: 1.065 ± 0.025	5.9LeuAsp: 5.9 ± 0.067	7.098LeuGlu: 7.098 ± 0.074	5.249LeuPhe: 5.249 ± 0.058	6.503LeuGly: 6.503 ± 0.074	1.7LeuHis: 1.7 ± 0.03	8.162LeuIle: 8.162 ± 0.092	7.424LeuLys: 7.424 ± 0.073	10.506LeuLeu: 10.506 ± 0.114	2.892LeuMet: 2.892 ± 0.046	4.942LeuAsn: 4.942 ± 0.054	4.368LeuPro: 4.368 ± 0.055	3.142LeuGln: 3.142 ± 0.046	4.157LeuArg: 4.157 ± 0.06	8.681LeuSer: 8.681 ± 0.083	5.304LeuThr: 5.304 ± 0.059	5.478LeuVal: 5.478 ± 0.059	1.042LeuTrp: 1.042 ± 0.03	3.502LeuTyr: 3.502 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	2.197MetAla: 2.197 ± 0.037	0.174MetCys: 0.174 ± 0.011	1.932MetAsp: 1.932 ± 0.034	2.206MetGlu: 2.206 ± 0.041	0.923MetPhe: 0.923 ± 0.022	2.127MetGly: 2.127 ± 0.039	0.389MetHis: 0.389 ± 0.014	2.318MetIle: 2.318 ± 0.039	2.534MetLys: 2.534 ± 0.035	2.224MetLeu: 2.224 ± 0.037	0.911MetMet: 0.911 ± 0.022	1.673MetAsn: 1.673 ± 0.031	1.045MetPro: 1.045 ± 0.024	0.731MetGln: 0.731 ± 0.02	1.079MetArg: 1.079 ± 0.024	1.934MetSer: 1.934 ± 0.037	1.747MetThr: 1.747 ± 0.032	1.827MetVal: 1.827 ± 0.033	0.185MetTrp: 0.185 ± 0.011	0.675MetTyr: 0.675 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.437AsnAla: 2.437 ± 0.039	0.512AsnCys: 0.512 ± 0.019	2.246AsnAsp: 2.246 ± 0.036	2.712AsnGlu: 2.712 ± 0.044	1.847AsnPhe: 1.847 ± 0.036	2.836AsnGly: 2.836 ± 0.04	0.898AsnHis: 0.898 ± 0.022	3.676AsnIle: 3.676 ± 0.054	2.755AsnLys: 2.755 ± 0.036	4.583AsnLeu: 4.583 ± 0.052	1.262AsnMet: 1.262 ± 0.024	2.065AsnAsn: 2.065 ± 0.04	2.435AsnPro: 2.435 ± 0.035	1.447AsnGln: 1.447 ± 0.029	2.418AsnArg: 2.418 ± 0.041	3.234AsnSer: 3.234 ± 0.051	2.192AsnThr: 2.192 ± 0.032	2.087AsnVal: 2.087 ± 0.035	0.608AsnTrp: 0.608 ± 0.018	1.692AsnTyr: 1.692 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	2.662ProAla: 2.662 ± 0.044	0.324ProCys: 0.324 ± 0.013	2.781ProAsp: 2.781 ± 0.045	4.013ProGlu: 4.013 ± 0.053	1.927ProPhe: 1.927 ± 0.03	3.371ProGly: 3.371 ± 0.048	0.736ProHis: 0.736 ± 0.018	2.096ProIle: 2.096 ± 0.039	1.265ProLys: 1.265 ± 0.027	4.132ProLeu: 4.132 ± 0.053	0.934ProMet: 0.934 ± 0.022	1.054ProAsn: 1.054 ± 0.027	1.282ProPro: 1.282 ± 0.029	1.076ProGln: 1.076 ± 0.026	1.469ProArg: 1.469 ± 0.026	2.583ProSer: 2.583 ± 0.041	1.363ProThr: 1.363 ± 0.028	3.253ProVal: 3.253 ± 0.048	0.562ProTrp: 0.562 ± 0.019	1.408ProTyr: 1.408 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	2.058GlnAla: 2.058 ± 0.041	0.216GlnCys: 0.216 ± 0.013	1.776GlnAsp: 1.776 ± 0.035	2.189GlnGlu: 2.189 ± 0.032	1.395GlnPhe: 1.395 ± 0.029	2.067GlnGly: 2.067 ± 0.038	0.44GlnHis: 0.44 ± 0.013	2.539GlnIle: 2.539 ± 0.038	2.329GlnLys: 2.329 ± 0.039	2.77GlnLeu: 2.77 ± 0.046	0.948GlnMet: 0.948 ± 0.022	1.647GlnAsn: 1.647 ± 0.028	0.904GlnPro: 0.904 ± 0.024	1.056GlnGln: 1.056 ± 0.032	1.339GlnArg: 1.339 ± 0.029	2.117GlnSer: 2.117 ± 0.038	1.45GlnThr: 1.45 ± 0.027	1.693GlnVal: 1.693 ± 0.03	0.36GlnTrp: 0.36 ± 0.014	1.09GlnTyr: 1.09 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	2.58ArgAla: 2.58 ± 0.039	0.44ArgCys: 0.44 ± 0.017	2.777ArgAsp: 2.777 ± 0.043	3.772ArgGlu: 3.772 ± 0.05	2.228ArgPhe: 2.228 ± 0.037	2.867ArgGly: 2.867 ± 0.047	0.75ArgHis: 0.75 ± 0.022	4.102ArgIle: 4.102 ± 0.046	3.423ArgLys: 3.423 ± 0.046	4.335ArgLeu: 4.335 ± 0.056	1.445ArgMet: 1.445 ± 0.032	2.313ArgAsn: 2.313 ± 0.041	1.395ArgPro: 1.395 ± 0.031	1.575ArgGln: 1.575 ± 0.027	2.136ArgArg: 2.136 ± 0.042	3.015ArgSer: 3.015 ± 0.043	2.224ArgThr: 2.224 ± 0.035	2.678ArgVal: 2.678 ± 0.041	0.54ArgTrp: 0.54 ± 0.018	1.767ArgTyr: 1.767 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	4.564SerAla: 4.564 ± 0.058	0.93SerCys: 0.93 ± 0.022	4.282SerAsp: 4.282 ± 0.061	4.976SerGlu: 4.976 ± 0.057	4.032SerPhe: 4.032 ± 0.048	5.927SerGly: 5.927 ± 0.066	1.361SerHis: 1.361 ± 0.028	5.73SerIle: 5.73 ± 0.062	3.219SerLys: 3.219 ± 0.045	8.393SerLeu: 8.393 ± 0.077	2.141SerMet: 2.141 ± 0.037	2.52SerAsn: 2.52 ± 0.04	2.75SerPro: 2.75 ± 0.044	2.098SerGln: 2.098 ± 0.036	4.016SerArg: 4.016 ± 0.052	5.812SerSer: 5.812 ± 0.082	3.172SerThr: 3.172 ± 0.045	4.586SerVal: 4.586 ± 0.053	1.022SerTrp: 1.022 ± 0.027	2.768SerTyr: 2.768 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	4.146ThrAla: 4.146 ± 0.055	0.337ThrCys: 0.337 ± 0.013	2.853ThrAsp: 2.853 ± 0.037	3.326ThrGlu: 3.326 ± 0.043	2.023ThrPhe: 2.023 ± 0.037	4.353ThrGly: 4.353 ± 0.056	0.848ThrHis: 0.848 ± 0.023	3.775ThrIle: 3.775 ± 0.052	2.014ThrLys: 2.014 ± 0.034	5.375ThrLeu: 5.375 ± 0.057	1.334ThrMet: 1.334 ± 0.027	1.58ThrAsn: 1.58 ± 0.032	2.203ThrPro: 2.203 ± 0.039	1.291ThrGln: 1.291 ± 0.026	1.949ThrArg: 1.949 ± 0.031	3.058ThrSer: 3.058 ± 0.047	2.347ThrThr: 2.347 ± 0.046	3.929ThrVal: 3.929 ± 0.053	0.48ThrTrp: 0.48 ± 0.018	1.395ThrTyr: 1.395 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	3.396ValAla: 3.396 ± 0.056	0.691ValCys: 0.691 ± 0.021	3.417ValAsp: 3.417 ± 0.046	4.017ValGlu: 4.017 ± 0.049	3.074ValPhe: 3.074 ± 0.045	3.278ValGly: 3.278 ± 0.04	1.073ValHis: 1.073 ± 0.029	4.668ValIle: 4.668 ± 0.057	3.605ValLys: 3.605 ± 0.048	6.884ValLeu: 6.884 ± 0.074	1.717ValMet: 1.717 ± 0.032	2.596ValAsn: 2.596 ± 0.042	2.421ValPro: 2.421 ± 0.038	1.872ValGln: 1.872 ± 0.03	2.679ValArg: 2.679 ± 0.041	4.653ValSer: 4.653 ± 0.06	2.859ValThr: 2.859 ± 0.041	3.596ValVal: 3.596 ± 0.052	0.55ValTrp: 0.55 ± 0.017	2.103ValTyr: 2.103 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	0.63TrpAla: 0.63 ± 0.018	0.12TrpCys: 0.12 ± 0.009	0.767TrpAsp: 0.767 ± 0.022	0.771TrpGlu: 0.771 ± 0.022	0.546TrpPhe: 0.546 ± 0.018	0.842TrpGly: 0.842 ± 0.023	0.207TrpHis: 0.207 ± 0.011	0.85TrpIle: 0.85 ± 0.024	0.786TrpLys: 0.786 ± 0.019	1.018TrpLeu: 1.018 ± 0.027	0.333TrpMet: 0.333 ± 0.012	0.714TrpAsn: 0.714 ± 0.022	0.356TrpPro: 0.356 ± 0.015	0.416TrpGln: 0.416 ± 0.016	0.445TrpArg: 0.445 ± 0.017	0.791TrpSer: 0.791 ± 0.022	0.59TrpThr: 0.59 ± 0.021	0.599TrpVal: 0.599 ± 0.019	0.14TrpTrp: 0.14 ± 0.011	0.416TrpTyr: 0.416 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.953TyrAla: 1.953 ± 0.036	0.415TyrCys: 0.415 ± 0.016	2.017TyrAsp: 2.017 ± 0.032	2.06TyrGlu: 2.06 ± 0.033	1.866TyrPhe: 1.866 ± 0.035	2.444TyrGly: 2.444 ± 0.04	0.705TyrHis: 0.705 ± 0.018	2.512TyrIle: 2.512 ± 0.04	2.157TyrLys: 2.157 ± 0.037	3.775TyrLeu: 3.775 ± 0.054	0.962TyrMet: 0.962 ± 0.023	1.711TyrAsn: 1.711 ± 0.035	1.651TyrPro: 1.651 ± 0.032	1.165TyrGln: 1.165 ± 0.032	2.035TyrArg: 2.035 ± 0.035	2.933TyrSer: 2.933 ± 0.046	1.84TyrThr: 1.84 ± 0.034	1.584TyrVal: 1.584 ± 0.03	0.462TyrTrp: 0.462 ± 0.017	1.522TyrTyr: 1.522 ± 0.034	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5141 proteins (1776840 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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