Cohnella lupini

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Cohnella

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5622 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D9IQ77|A0A3D9IQ77_9BACL Nucleoside-binding protein OS=Cohnella lupini OX=1294267 GN=DFP95_103175 PE=3 SV=1
MM1 pKa = 7.46AVIICPWCQSEE12 pKa = 4.5IIQEE16 pKa = 4.09EE17 pKa = 4.49GQEE20 pKa = 4.07PEE22 pKa = 4.77KK23 pKa = 10.71ICPVCDD29 pKa = 3.65NEE31 pKa = 4.2LDD33 pKa = 3.86GYY35 pKa = 9.62RR36 pKa = 11.84TLKK39 pKa = 10.91LNIGEE44 pKa = 4.19EE45 pKa = 4.3DD46 pKa = 3.62EE47 pKa = 6.4DD48 pKa = 5.09DD49 pKa = 6.1DD50 pKa = 5.64EE51 pKa = 6.99DD52 pKa = 5.59EE53 pKa = 5.22EE54 pKa = 6.18DD55 pKa = 5.38FDD57 pKa = 6.39DD58 pKa = 5.02EE59 pKa = 4.41DD60 pKa = 4.51RR61 pKa = 11.84EE62 pKa = 4.26YY63 pKa = 11.03EE64 pKa = 4.19DD65 pKa = 5.05RR66 pKa = 11.84SSAPMVSIDD75 pKa = 5.16EE76 pKa = 4.91DD77 pKa = 3.82IAWVDD82 pKa = 3.84DD83 pKa = 4.06EE84 pKa = 5.65SLIEE88 pKa = 4.46PDD90 pKa = 4.13EE91 pKa = 4.2EE92 pKa = 3.93LQQFEE97 pKa = 4.62EE98 pKa = 4.62TVEE101 pKa = 3.89QLLNEE106 pKa = 4.12QEE108 pKa = 4.54VVPEE112 pKa = 4.34CPSCHH117 pKa = 6.27EE118 pKa = 3.92YY119 pKa = 10.14MIEE122 pKa = 4.12FGEE125 pKa = 4.26QLVSAPQFLPRR136 pKa = 11.84TAEE139 pKa = 4.02ALGEE143 pKa = 4.4TVLEE147 pKa = 4.4APFTLTMYY155 pKa = 9.72VCPSCFTVQSFLGDD169 pKa = 3.48SHH171 pKa = 6.9RR172 pKa = 11.84HH173 pKa = 3.79QLVRR177 pKa = 11.84RR178 pKa = 11.84LSQAPGSGEE187 pKa = 3.79RR188 pKa = 11.84EE189 pKa = 3.85RR190 pKa = 11.84EE191 pKa = 4.01VV192 pKa = 3.14

Molecular weight: 22.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D9IT36|A0A3D9IT36_9BACL Acetoin utilization transport system permease protein OS=Cohnella lupini OX=1294267 GN=DFP95_102352 PE=4 SV=1
MM1 pKa = 6.94IVVRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84VKK9 pKa = 10.35AGARR13 pKa = 11.84LVPRR17 pKa = 11.84YY18 pKa = 9.66RR19 pKa = 11.84GRR21 pKa = 11.84LAGAPSVGGAKK32 pKa = 10.0RR33 pKa = 11.84KK34 pKa = 9.24ILKK37 pKa = 9.01TSLWLRR43 pKa = 11.84KK44 pKa = 9.56GKK46 pKa = 10.23

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5622

0

5622

1863763

28

4595

331.5

36.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.225 ± 0.038

0.684 ± 0.01

5.366 ± 0.028

6.549 ± 0.036

4.178 ± 0.023

7.564 ± 0.028

1.913 ± 0.013

6.744 ± 0.031

5.147 ± 0.027

9.968 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.718 ± 0.017

4.023 ± 0.025

4.1 ± 0.021

3.462 ± 0.019

5.276 ± 0.033

6.716 ± 0.026

5.477 ± 0.036

7.126 ± 0.027

1.342 ± 0.014

3.422 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski