Acinetobacter phage ABPH49

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; unclassified Vequintavirinae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 243 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346FIM6|A0A346FIM6_9CAUD Uncharacterized protein OS=Acinetobacter phage ABPH49 OX=2282400 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 4.05YY3 pKa = 9.98VANVMVVGSNGDD15 pKa = 3.54LVAFKK20 pKa = 10.54AYY22 pKa = 10.47GPFKK26 pKa = 10.29SYY28 pKa = 10.65EE29 pKa = 4.03YY30 pKa = 10.86AAGIAQKK37 pKa = 10.84EE38 pKa = 4.4VMNLWTDD45 pKa = 3.79LCLDD49 pKa = 3.59DD50 pKa = 4.76TGLSFSSSVVFGEE63 pKa = 4.01DD64 pKa = 3.43LEE66 pKa = 5.43LEE68 pKa = 4.42ALSGPLPKK76 pKa = 10.12EE77 pKa = 4.0VPDD80 pKa = 3.85AVV82 pKa = 3.44

Molecular weight: 8.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346FIV0|A0A346FIV0_9CAUD RNA ligase 2 OS=Acinetobacter phage ABPH49 OX=2282400 PE=4 SV=1
MM1 pKa = 7.72WEE3 pKa = 4.12QIPNLVINHH12 pKa = 5.87YY13 pKa = 10.53SNPATSRR20 pKa = 11.84RR21 pKa = 11.84IRR23 pKa = 11.84SPALLISDD31 pKa = 4.72RR32 pKa = 11.84GRR34 pKa = 11.84SGYY37 pKa = 8.36TEE39 pKa = 4.37FTRR42 pKa = 11.84VTASSQLQNWLFAKK56 pKa = 10.5

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

243

0

243

39431

38

960

162.3

18.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.479 ± 0.158

1.159 ± 0.079

6.68 ± 0.121

7.375 ± 0.27

3.969 ± 0.128

7.509 ± 0.325

2.001 ± 0.085

5.658 ± 0.129

6.936 ± 0.165

7.598 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.137 ± 0.116

4.39 ± 0.1

3.812 ± 0.131

3.388 ± 0.103

5.212 ± 0.153

5.851 ± 0.16

5.486 ± 0.183

7.157 ± 0.152

1.621 ± 0.077

3.583 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski