Actinobacteria bacterium OV320

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; unclassified Actinomycetia

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9056 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1GK75|A0A0N1GK75_9ACTN ABC-type transporter integral membrane subunit OS=Actinobacteria bacterium OV320 OX=1592329 GN=OV320_7245 PE=3 SV=1
MM1 pKa = 7.81SIPPPPGPHH10 pKa = 5.88QPQDD14 pKa = 3.84PYY16 pKa = 11.35QPPQPQSPYY25 pKa = 9.04PQDD28 pKa = 3.62PFAPPPQDD36 pKa = 3.86PYY38 pKa = 11.7AQGPYY43 pKa = 10.17GPQGPQAPMGPGPMGPGPMGPGPGPMGPYY72 pKa = 8.93PHH74 pKa = 7.58PSYY77 pKa = 10.58PQAPYY82 pKa = 10.61GAVPYY87 pKa = 8.8PVWGQGYY94 pKa = 9.06SPYY97 pKa = 10.63GRR99 pKa = 11.84PSPVNGVAIAALVLGLLCFLPGVGLLLGIIALVQIRR135 pKa = 11.84KK136 pKa = 9.57RR137 pKa = 11.84GEE139 pKa = 3.57RR140 pKa = 11.84GKK142 pKa = 11.14AMAIVGTVLSSVGLAVWVLALSTGGASDD170 pKa = 3.57FWDD173 pKa = 4.08GFKK176 pKa = 10.72EE177 pKa = 4.31GASGNSSFALAKK189 pKa = 10.13GDD191 pKa = 3.93CFDD194 pKa = 4.22VPGEE198 pKa = 4.19TFDD201 pKa = 3.85EE202 pKa = 4.34DD203 pKa = 4.44VYY205 pKa = 11.81DD206 pKa = 4.06VDD208 pKa = 5.54EE209 pKa = 4.46VDD211 pKa = 4.93CSGEE215 pKa = 3.54HH216 pKa = 6.53DD217 pKa = 3.83AEE219 pKa = 4.55VFASVPLTGDD229 pKa = 3.45SFPGDD234 pKa = 3.42DD235 pKa = 4.44HH236 pKa = 6.23VTDD239 pKa = 3.76VADD242 pKa = 4.43DD243 pKa = 3.47KK244 pKa = 11.7CYY246 pKa = 10.12TLQDD250 pKa = 4.01AYY252 pKa = 11.05AMDD255 pKa = 4.25PWALTDD261 pKa = 4.07EE262 pKa = 4.14VDD264 pKa = 4.09IYY266 pKa = 11.52YY267 pKa = 7.57LTPTADD273 pKa = 2.79SWSWGDD279 pKa = 4.3RR280 pKa = 11.84EE281 pKa = 4.37ITCVFANVDD290 pKa = 3.61EE291 pKa = 5.11KK292 pKa = 11.33GTLTGSLRR300 pKa = 11.84ADD302 pKa = 3.72ASTLDD307 pKa = 3.66PDD309 pKa = 3.55QFAFLKK315 pKa = 10.93AMAAVDD321 pKa = 3.68DD322 pKa = 4.28VLFEE326 pKa = 4.23EE327 pKa = 4.77PEE329 pKa = 4.1EE330 pKa = 4.06YY331 pKa = 10.71AEE333 pKa = 5.31EE334 pKa = 4.41DD335 pKa = 3.52LASNRR340 pKa = 11.84TWAGDD345 pKa = 3.39TGEE348 pKa = 4.34VTGNQADD355 pKa = 3.62QLGSHH360 pKa = 6.4LWEE363 pKa = 4.59EE364 pKa = 4.63PDD366 pKa = 4.41RR367 pKa = 11.84EE368 pKa = 4.36APLAALVKK376 pKa = 10.64DD377 pKa = 3.43MRR379 pKa = 11.84TASKK383 pKa = 10.06EE384 pKa = 3.39WAAAAKK390 pKa = 10.22AADD393 pKa = 3.39ADD395 pKa = 4.13GFYY398 pKa = 10.85EE399 pKa = 5.18HH400 pKa = 7.27YY401 pKa = 10.55DD402 pKa = 3.22KK403 pKa = 11.04AYY405 pKa = 9.93KK406 pKa = 10.33YY407 pKa = 10.85VDD409 pKa = 3.18GATTVTARR417 pKa = 11.84KK418 pKa = 9.59ALGLATTVPTYY429 pKa = 11.03DD430 pKa = 3.86EE431 pKa = 5.75DD432 pKa = 4.39SDD434 pKa = 4.15SGSGGTGGGDD444 pKa = 3.25SDD446 pKa = 5.04SGLDD450 pKa = 3.39VV451 pKa = 3.72

Molecular weight: 47.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N1GRG6|A0A0N1GRG6_9ACTN Putative anti-sigma regulatory factor serine/threonine protein kinase OS=Actinobacteria bacterium OV320 OX=1592329 GN=OV320_6689 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 11.13GRR40 pKa = 11.84TNLSAA45 pKa = 4.57

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9056

0

9056

2938333

30

7352

324.5

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.522 ± 0.045

0.781 ± 0.007

6.019 ± 0.021

5.622 ± 0.024

2.711 ± 0.015

9.455 ± 0.025

2.307 ± 0.012

3.11 ± 0.017

2.168 ± 0.021

10.237 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.681 ± 0.011

1.817 ± 0.017

6.104 ± 0.026

2.791 ± 0.016

7.973 ± 0.029

5.196 ± 0.027

6.358 ± 0.026

8.471 ± 0.025

1.544 ± 0.01

2.135 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski