Desulfoluna spongiiphila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Desulfoluna

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5238 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5D6N5|A0A1G5D6N5_9DELT Long-chain fatty acid transport protein OS=Desulfoluna spongiiphila OX=419481 GN=SAMN05216233_10410 PE=3 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84KK3 pKa = 8.87PLGPIGTLLCRR14 pKa = 11.84CLSYY18 pKa = 10.93LAGSPARR25 pKa = 11.84TGIRR29 pKa = 11.84GLSLLVALLALPCVASGMEE48 pKa = 4.31ALSEE52 pKa = 4.02HH53 pKa = 5.38QMKK56 pKa = 8.8GTTAQAGISIAMDD69 pKa = 3.45DD70 pKa = 3.6VTFYY74 pKa = 10.96HH75 pKa = 6.67HH76 pKa = 7.14VDD78 pKa = 3.25AFRR81 pKa = 11.84LTDD84 pKa = 4.08PDD86 pKa = 4.24DD87 pKa = 4.2PSGYY91 pKa = 9.22MEE93 pKa = 4.33FQDD96 pKa = 4.41IEE98 pKa = 4.53SLTTIDD104 pKa = 4.75VGTSDD109 pKa = 4.15VNGDD113 pKa = 3.48NTIGCITLDD122 pKa = 3.82LFTVSDD128 pKa = 3.76ATSPINGKK136 pKa = 8.77PLLYY140 pKa = 10.64LVGADD145 pKa = 2.86IDD147 pKa = 4.31YY148 pKa = 11.49NKK150 pKa = 10.63DD151 pKa = 3.22LTIGAINVCGTDD163 pKa = 2.89IGSAAVDD170 pKa = 3.76NYY172 pKa = 11.52AMPSFHH178 pKa = 7.25LYY180 pKa = 10.51LGAHH184 pKa = 6.82DD185 pKa = 4.99CGVDD189 pKa = 3.46LEE191 pKa = 4.62FGARR195 pKa = 11.84ITMDD199 pKa = 2.99QFSYY203 pKa = 9.23GTSPDD208 pKa = 3.52DD209 pKa = 3.43TLTLSGITLAGSFTDD224 pKa = 4.61NPADD228 pKa = 3.96TPSDD232 pKa = 3.54PSTWQAGGEE241 pKa = 4.15FVLGDD246 pKa = 3.57IASGNPLTIDD256 pKa = 3.3VATDD260 pKa = 3.7TTASWADD267 pKa = 3.68DD268 pKa = 3.74ADD270 pKa = 4.27GNPIPNPRR278 pKa = 11.84LGSGFIAINMPMEE291 pKa = 4.48GSLRR295 pKa = 11.84IEE297 pKa = 3.86NMGFGTNDD305 pKa = 3.31FGALALDD312 pKa = 4.74NIQAHH317 pKa = 6.57KK318 pKa = 10.54LYY320 pKa = 10.44IEE322 pKa = 4.11IPGRR326 pKa = 11.84GLGVPP331 pKa = 4.21

Molecular weight: 34.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5ALR7|A0A1G5ALR7_9DELT Phosphoglycolate phosphatase OS=Desulfoluna spongiiphila OX=419481 GN=SAMN05216233_101285 PE=4 SV=1
MM1 pKa = 7.5RR2 pKa = 11.84WRR4 pKa = 11.84WLSALALMVRR14 pKa = 11.84RR15 pKa = 11.84LVYY18 pKa = 9.84PPRR21 pKa = 11.84CRR23 pKa = 11.84VCGVLLPWEE32 pKa = 4.17RR33 pKa = 11.84VGGSSSLHH41 pKa = 6.29SGDD44 pKa = 3.92LASMFHH50 pKa = 7.16GEE52 pKa = 4.36LCHH55 pKa = 7.23VMCATCRR62 pKa = 11.84AGVQEE67 pKa = 4.73PSGQRR72 pKa = 11.84CTACGQPLSGVVFDD86 pKa = 5.45PPLCGPCMDD95 pKa = 5.33PDD97 pKa = 3.89RR98 pKa = 11.84PLLRR102 pKa = 11.84MTALYY107 pKa = 10.52LHH109 pKa = 7.34DD110 pKa = 5.59GGPAMAVRR118 pKa = 11.84ALKK121 pKa = 10.49YY122 pKa = 9.77KK123 pKa = 10.41NKK125 pKa = 9.92RR126 pKa = 11.84ALAPPMGRR134 pKa = 11.84LLFARR139 pKa = 11.84ALRR142 pKa = 11.84AHH144 pKa = 7.28LDD146 pKa = 3.76GEE148 pKa = 4.35AKK150 pKa = 10.27RR151 pKa = 11.84WDD153 pKa = 3.38IDD155 pKa = 3.76LVVPVPLHH163 pKa = 5.63GKK165 pKa = 8.57RR166 pKa = 11.84LKK168 pKa = 10.92ARR170 pKa = 11.84GFNQASVLLAAFEE183 pKa = 4.46KK184 pKa = 10.57EE185 pKa = 3.51AGLRR189 pKa = 11.84VCHH192 pKa = 6.32RR193 pKa = 11.84LLRR196 pKa = 11.84RR197 pKa = 11.84RR198 pKa = 11.84RR199 pKa = 11.84PTTPQAGLSRR209 pKa = 11.84RR210 pKa = 11.84ARR212 pKa = 11.84LTNVKK217 pKa = 10.04GAFEE221 pKa = 4.26LAPGRR226 pKa = 11.84MVEE229 pKa = 4.08GLKK232 pKa = 10.65VLLVDD237 pKa = 4.96DD238 pKa = 5.09VYY240 pKa = 9.5TTGSTLAACAALLKK254 pKa = 10.5RR255 pKa = 11.84HH256 pKa = 5.92GATEE260 pKa = 3.98VSALVFARR268 pKa = 11.84RR269 pKa = 11.84DD270 pKa = 3.42MTHH273 pKa = 6.05

Molecular weight: 30.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5238

0

5238

1765537

25

4215

337.1

37.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.365 ± 0.04

1.309 ± 0.015

5.541 ± 0.031

6.559 ± 0.033

4.171 ± 0.023

8.205 ± 0.03

2.204 ± 0.016

5.694 ± 0.032

4.939 ± 0.034

9.879 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.898 ± 0.018

3.122 ± 0.021

4.582 ± 0.023

2.684 ± 0.016

5.762 ± 0.029

5.924 ± 0.026

5.876 ± 0.028

7.391 ± 0.032

1.133 ± 0.014

2.762 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski