Tomato yellow leaf curl Shuangbai virus - [Y4536]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato yellow leaf curl Shuangbai virus

Average proteome isoelectric point is 7.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A161GPN4|A0A161GPN4_9GEMI AC4 protein OS=Tomato yellow leaf curl Shuangbai virus - [Y4536] OX=1825931 GN=AC4 PE=3 SV=1
MM1 pKa = 7.71APPKK5 pKa = 10.28RR6 pKa = 11.84FQLNAKK12 pKa = 9.5HH13 pKa = 6.2YY14 pKa = 9.43FLTYY18 pKa = 8.51PQCSLTKK25 pKa = 10.58EE26 pKa = 4.02EE27 pKa = 5.64ALSQLQNLVTPTNKK41 pKa = 10.08KK42 pKa = 9.27YY43 pKa = 10.84IKK45 pKa = 9.66ICRR48 pKa = 11.84EE49 pKa = 3.74LHH51 pKa = 6.25EE52 pKa = 5.87DD53 pKa = 4.22GSPHH57 pKa = 6.11LHH59 pKa = 6.16VLIQFEE65 pKa = 4.37GKK67 pKa = 9.67YY68 pKa = 9.61RR69 pKa = 11.84CTNNRR74 pKa = 11.84FFDD77 pKa = 4.02LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.17VKK101 pKa = 11.17SYY103 pKa = 11.2LDD105 pKa = 3.52KK106 pKa = 11.64DD107 pKa = 3.53GDD109 pKa = 3.85TLEE112 pKa = 4.14WGEE115 pKa = 3.84FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQSANDD132 pKa = 4.12AYY134 pKa = 10.85AKK136 pKa = 10.51ALNSGSKK143 pKa = 10.2SEE145 pKa = 4.18ALNVLKK151 pKa = 10.57EE152 pKa = 4.26LAPKK156 pKa = 10.39DD157 pKa = 3.7YY158 pKa = 11.04VLQYY162 pKa = 11.35HH163 pKa = 6.21NLNSNLDD170 pKa = 4.01RR171 pKa = 11.84IFAPPIEE178 pKa = 4.54VFVCPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 4.0EE195 pKa = 4.15LEE197 pKa = 3.55KK198 pKa = 10.16WAAEE202 pKa = 4.15NVVSAAARR210 pKa = 11.84PWRR213 pKa = 11.84PKK215 pKa = 10.55SIVIEE220 pKa = 4.33GDD222 pKa = 3.1SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.26YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTSSYY311 pKa = 11.03KK312 pKa = 10.58EE313 pKa = 3.93YY314 pKa = 11.1LNEE317 pKa = 4.81DD318 pKa = 3.65KK319 pKa = 11.28NSALKK324 pKa = 10.56DD325 pKa = 3.37WAIKK329 pKa = 9.63NAEE332 pKa = 4.63FITINNPLYY341 pKa = 11.0SGTNQSATQNSQEE354 pKa = 4.27EE355 pKa = 4.3ASSQAEE361 pKa = 4.18SS362 pKa = 3.21

Molecular weight: 40.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A165U0X3|A0A165U0X3_9GEMI Replication enhancer OS=Tomato yellow leaf curl Shuangbai virus - [Y4536] OX=1825931 GN=AC3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 9.79VSRR30 pKa = 11.84AAAPIVRR37 pKa = 11.84VTKK40 pKa = 10.52ARR42 pKa = 11.84AWANRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.87PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.1SRR80 pKa = 11.84HH81 pKa = 6.13DD82 pKa = 3.26IQHH85 pKa = 6.35IGKK88 pKa = 8.97VMCVSDD94 pKa = 3.59VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.38NVHH172 pKa = 6.5RR173 pKa = 11.84DD174 pKa = 3.15RR175 pKa = 11.84YY176 pKa = 8.56QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.68KK201 pKa = 9.39FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.08YY219 pKa = 8.01EE220 pKa = 4.07NHH222 pKa = 6.29SEE224 pKa = 4.11NALMLYY230 pKa = 7.58MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight: 29.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1105

100

362

184.2

21.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.52 ± 0.94

2.534 ± 0.558

4.887 ± 0.374

4.796 ± 0.612

4.163 ± 0.418

4.253 ± 0.391

4.072 ± 0.717

5.339 ± 0.862

5.611 ± 0.939

6.606 ± 0.935

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.991 ± 0.56

6.244 ± 0.522

6.063 ± 0.559

5.158 ± 0.645

6.968 ± 1.208

7.873 ± 1.107

6.154 ± 0.984

6.244 ± 1.241

1.629 ± 0.241

3.891 ± 0.426
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski