Amino acid dipepetide frequency for Bacillus phage BtiUFT6.51-F

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.191AlaAla: 5.191 ± 1.044
0.586AlaCys: 0.586 ± 0.281
3.684AlaAsp: 3.684 ± 0.674
4.187AlaGlu: 4.187 ± 0.713
2.261AlaPhe: 2.261 ± 0.436
3.852AlaGly: 3.852 ± 0.771
0.754AlaHis: 0.754 ± 0.237
4.019AlaIle: 4.019 ± 0.488
6.698AlaLys: 6.698 ± 0.709
5.61AlaLeu: 5.61 ± 0.787
2.093AlaMet: 2.093 ± 0.372
3.517AlaAsn: 3.517 ± 0.607
1.758AlaPro: 1.758 ± 0.274
2.093AlaGln: 2.093 ± 0.475
3.098AlaArg: 3.098 ± 0.452
3.098AlaSer: 3.098 ± 0.708
4.187AlaThr: 4.187 ± 0.849
4.103AlaVal: 4.103 ± 0.788
0.586AlaTrp: 0.586 ± 0.201
2.177AlaTyr: 2.177 ± 0.453
0.0AlaXaa: 0.0 ± 0.0
Cys
0.419CysAla: 0.419 ± 0.204
0.084CysCys: 0.084 ± 0.088
1.005CysAsp: 1.005 ± 0.301
0.586CysGlu: 0.586 ± 0.219
0.67CysPhe: 0.67 ± 0.228
0.586CysGly: 0.586 ± 0.232
0.335CysHis: 0.335 ± 0.191
0.335CysIle: 0.335 ± 0.148
0.586CysLys: 0.586 ± 0.245
0.419CysLeu: 0.419 ± 0.203
0.502CysMet: 0.502 ± 0.229
0.335CysAsn: 0.335 ± 0.214
0.335CysPro: 0.335 ± 0.174
0.251CysGln: 0.251 ± 0.145
0.586CysArg: 0.586 ± 0.193
0.67CysSer: 0.67 ± 0.231
0.419CysThr: 0.419 ± 0.156
0.419CysVal: 0.419 ± 0.16
0.0CysTrp: 0.0 ± 0.0
0.335CysTyr: 0.335 ± 0.184
0.0CysXaa: 0.0 ± 0.0
Asp
3.266AspAla: 3.266 ± 0.638
0.251AspCys: 0.251 ± 0.127
2.847AspAsp: 2.847 ± 0.471
5.108AspGlu: 5.108 ± 0.643
2.344AspPhe: 2.344 ± 0.394
3.6AspGly: 3.6 ± 0.485
0.754AspHis: 0.754 ± 0.205
4.27AspIle: 4.27 ± 0.538
4.856AspLys: 4.856 ± 0.447
5.108AspLeu: 5.108 ± 0.641
2.344AspMet: 2.344 ± 0.408
2.177AspAsn: 2.177 ± 0.428
1.423AspPro: 1.423 ± 0.356
1.675AspGln: 1.675 ± 0.376
2.261AspArg: 2.261 ± 0.354
2.596AspSer: 2.596 ± 0.303
2.428AspThr: 2.428 ± 0.388
3.6AspVal: 3.6 ± 0.529
0.754AspTrp: 0.754 ± 0.204
2.512AspTyr: 2.512 ± 0.426
0.0AspXaa: 0.0 ± 0.0
Glu
5.945GluAla: 5.945 ± 0.695
0.837GluCys: 0.837 ± 0.269
3.517GluAsp: 3.517 ± 0.607
6.866GluGlu: 6.866 ± 0.663
4.187GluPhe: 4.187 ± 0.522
4.187GluGly: 4.187 ± 0.529
1.926GluHis: 1.926 ± 0.436
7.536GluIle: 7.536 ± 0.649
8.541GluLys: 8.541 ± 0.642
7.285GluLeu: 7.285 ± 0.793
2.512GluMet: 2.512 ± 0.412
4.103GluAsn: 4.103 ± 0.549
1.758GluPro: 1.758 ± 0.461
4.354GluGln: 4.354 ± 0.509
3.935GluArg: 3.935 ± 0.638
3.852GluSer: 3.852 ± 0.584
3.6GluThr: 3.6 ± 0.542
4.521GluVal: 4.521 ± 0.745
0.837GluTrp: 0.837 ± 0.285
2.261GluTyr: 2.261 ± 0.47
0.0GluXaa: 0.0 ± 0.0
Phe
2.344PheAla: 2.344 ± 0.399
0.335PheCys: 0.335 ± 0.168
2.344PheAsp: 2.344 ± 0.438
2.931PheGlu: 2.931 ± 0.522
1.172PhePhe: 1.172 ± 0.382
3.684PheGly: 3.684 ± 0.458
0.754PheHis: 0.754 ± 0.243
3.014PheIle: 3.014 ± 0.505
3.684PheLys: 3.684 ± 0.589
4.019PheLeu: 4.019 ± 0.7
1.591PheMet: 1.591 ± 0.358
3.014PheAsn: 3.014 ± 0.447
0.754PhePro: 0.754 ± 0.253
1.675PheGln: 1.675 ± 0.315
1.926PheArg: 1.926 ± 0.399
2.344PheSer: 2.344 ± 0.421
2.177PheThr: 2.177 ± 0.423
2.177PheVal: 2.177 ± 0.327
0.67PheTrp: 0.67 ± 0.233
1.423PheTyr: 1.423 ± 0.251
0.0PheXaa: 0.0 ± 0.0
Gly
2.763GlyAla: 2.763 ± 0.651
0.251GlyCys: 0.251 ± 0.14
2.763GlyAsp: 2.763 ± 0.401
5.024GlyGlu: 5.024 ± 0.591
2.512GlyPhe: 2.512 ± 0.526
2.763GlyGly: 2.763 ± 0.456
0.754GlyHis: 0.754 ± 0.227
3.6GlyIle: 3.6 ± 0.544
5.694GlyLys: 5.694 ± 0.741
4.773GlyLeu: 4.773 ± 0.749
2.512GlyMet: 2.512 ± 0.611
2.512GlyAsn: 2.512 ± 0.361
0.586GlyPro: 0.586 ± 0.203
1.758GlyGln: 1.758 ± 0.291
2.763GlyArg: 2.763 ± 0.527
3.433GlySer: 3.433 ± 0.449
2.763GlyThr: 2.763 ± 0.368
4.521GlyVal: 4.521 ± 0.564
1.005GlyTrp: 1.005 ± 0.336
2.344GlyTyr: 2.344 ± 0.362
0.0GlyXaa: 0.0 ± 0.0
His
0.837HisAla: 0.837 ± 0.282
0.419HisCys: 0.419 ± 0.205
0.502HisAsp: 0.502 ± 0.213
1.423HisGlu: 1.423 ± 0.306
0.67HisPhe: 0.67 ± 0.25
1.005HisGly: 1.005 ± 0.257
0.251HisHis: 0.251 ± 0.148
0.837HisIle: 0.837 ± 0.21
2.01HisLys: 2.01 ± 0.499
1.758HisLeu: 1.758 ± 0.375
0.586HisMet: 0.586 ± 0.188
1.005HisAsn: 1.005 ± 0.332
0.335HisPro: 0.335 ± 0.189
0.754HisGln: 0.754 ± 0.28
0.754HisArg: 0.754 ± 0.247
1.842HisSer: 1.842 ± 0.391
1.256HisThr: 1.256 ± 0.406
0.837HisVal: 0.837 ± 0.247
0.335HisTrp: 0.335 ± 0.144
0.419HisTyr: 0.419 ± 0.161
0.0HisXaa: 0.0 ± 0.0
Ile
5.777IleAla: 5.777 ± 0.65
0.586IleCys: 0.586 ± 0.195
3.433IleAsp: 3.433 ± 0.583
7.285IleGlu: 7.285 ± 0.822
2.344IlePhe: 2.344 ± 0.44
3.098IleGly: 3.098 ± 0.61
1.423IleHis: 1.423 ± 0.381
4.773IleIle: 4.773 ± 0.647
5.945IleLys: 5.945 ± 0.542
4.27IleLeu: 4.27 ± 0.543
2.093IleMet: 2.093 ± 0.47
5.191IleAsn: 5.191 ± 0.696
2.177IlePro: 2.177 ± 0.405
3.433IleGln: 3.433 ± 0.537
3.098IleArg: 3.098 ± 0.456
3.852IleSer: 3.852 ± 0.525
4.773IleThr: 4.773 ± 0.53
3.768IleVal: 3.768 ± 0.55
0.754IleTrp: 0.754 ± 0.247
2.344IleTyr: 2.344 ± 0.538
0.0IleXaa: 0.0 ± 0.0
Lys
6.615LysAla: 6.615 ± 0.669
0.67LysCys: 0.67 ± 0.231
4.521LysAsp: 4.521 ± 0.503
7.954LysGlu: 7.954 ± 0.78
3.684LysPhe: 3.684 ± 0.419
3.182LysGly: 3.182 ± 0.538
1.591LysHis: 1.591 ± 0.299
5.861LysIle: 5.861 ± 0.529
9.043LysLys: 9.043 ± 0.991
6.95LysLeu: 6.95 ± 0.714
3.014LysMet: 3.014 ± 0.539
5.526LysAsn: 5.526 ± 0.602
2.679LysPro: 2.679 ± 0.47
5.861LysGln: 5.861 ± 0.754
5.61LysArg: 5.61 ± 0.728
5.945LysSer: 5.945 ± 0.485
5.945LysThr: 5.945 ± 0.586
5.945LysVal: 5.945 ± 0.646
1.172LysTrp: 1.172 ± 0.328
3.266LysTyr: 3.266 ± 0.402
0.0LysXaa: 0.0 ± 0.0
Leu
6.196LeuAla: 6.196 ± 0.818
1.089LeuCys: 1.089 ± 0.367
4.94LeuAsp: 4.94 ± 0.6
6.531LeuGlu: 6.531 ± 0.744
2.596LeuPhe: 2.596 ± 0.467
4.438LeuGly: 4.438 ± 0.729
1.842LeuHis: 1.842 ± 0.408
5.275LeuIle: 5.275 ± 0.695
9.043LeuLys: 9.043 ± 0.984
5.275LeuLeu: 5.275 ± 0.786
1.172LeuMet: 1.172 ± 0.336
5.359LeuAsn: 5.359 ± 0.597
2.512LeuPro: 2.512 ± 0.432
3.6LeuGln: 3.6 ± 0.526
4.187LeuArg: 4.187 ± 0.555
5.61LeuSer: 5.61 ± 0.667
3.935LeuThr: 3.935 ± 0.581
4.521LeuVal: 4.521 ± 0.584
0.754LeuTrp: 0.754 ± 0.262
2.763LeuTyr: 2.763 ± 0.53
0.0LeuXaa: 0.0 ± 0.0
Met
1.34MetAla: 1.34 ± 0.31
0.251MetCys: 0.251 ± 0.155
1.675MetAsp: 1.675 ± 0.314
2.847MetGlu: 2.847 ± 0.435
1.005MetPhe: 1.005 ± 0.313
1.758MetGly: 1.758 ± 0.391
0.251MetHis: 0.251 ± 0.147
2.01MetIle: 2.01 ± 0.323
3.852MetLys: 3.852 ± 0.61
2.763MetLeu: 2.763 ± 0.508
0.921MetMet: 0.921 ± 0.289
1.758MetAsn: 1.758 ± 0.364
0.754MetPro: 0.754 ± 0.21
1.591MetGln: 1.591 ± 0.377
1.34MetArg: 1.34 ± 0.341
1.34MetSer: 1.34 ± 0.256
2.01MetThr: 2.01 ± 0.302
1.507MetVal: 1.507 ± 0.355
0.502MetTrp: 0.502 ± 0.156
0.921MetTyr: 0.921 ± 0.342
0.0MetXaa: 0.0 ± 0.0
Asn
3.935AsnAla: 3.935 ± 0.597
0.251AsnCys: 0.251 ± 0.144
2.679AsnAsp: 2.679 ± 0.547
4.689AsnGlu: 4.689 ± 0.557
2.01AsnPhe: 2.01 ± 0.439
3.517AsnGly: 3.517 ± 0.51
1.172AsnHis: 1.172 ± 0.271
4.605AsnIle: 4.605 ± 0.619
5.191AsnLys: 5.191 ± 0.631
4.187AsnLeu: 4.187 ± 0.734
1.758AsnMet: 1.758 ± 0.352
2.512AsnAsn: 2.512 ± 0.361
2.093AsnPro: 2.093 ± 0.498
2.596AsnGln: 2.596 ± 0.447
3.014AsnArg: 3.014 ± 0.494
2.931AsnSer: 2.931 ± 0.479
3.517AsnThr: 3.517 ± 0.43
3.098AsnVal: 3.098 ± 0.4
0.67AsnTrp: 0.67 ± 0.246
1.926AsnTyr: 1.926 ± 0.408
0.0AsnXaa: 0.0 ± 0.0
Pro
0.837ProAla: 0.837 ± 0.219
0.335ProCys: 0.335 ± 0.174
1.423ProAsp: 1.423 ± 0.377
2.093ProGlu: 2.093 ± 0.481
1.005ProPhe: 1.005 ± 0.333
1.256ProGly: 1.256 ± 0.329
0.837ProHis: 0.837 ± 0.192
2.679ProIle: 2.679 ± 0.515
2.093ProLys: 2.093 ± 0.487
2.177ProLeu: 2.177 ± 0.45
0.335ProMet: 0.335 ± 0.141
2.177ProAsn: 2.177 ± 0.418
1.005ProPro: 1.005 ± 0.354
0.837ProGln: 0.837 ± 0.349
0.586ProArg: 0.586 ± 0.213
2.093ProSer: 2.093 ± 0.403
2.01ProThr: 2.01 ± 0.387
1.507ProVal: 1.507 ± 0.515
0.502ProTrp: 0.502 ± 0.188
1.675ProTyr: 1.675 ± 0.356
0.0ProXaa: 0.0 ± 0.0
Gln
3.266GlnAla: 3.266 ± 0.804
0.335GlnCys: 0.335 ± 0.163
1.926GlnAsp: 1.926 ± 0.34
3.182GlnGlu: 3.182 ± 0.62
1.675GlnPhe: 1.675 ± 0.391
2.344GlnGly: 2.344 ± 0.456
0.837GlnHis: 0.837 ± 0.394
3.014GlnIle: 3.014 ± 0.421
5.443GlnLys: 5.443 ± 0.877
3.349GlnLeu: 3.349 ± 0.574
1.507GlnMet: 1.507 ± 0.345
2.177GlnAsn: 2.177 ± 0.495
1.34GlnPro: 1.34 ± 0.315
2.847GlnGln: 2.847 ± 0.507
2.093GlnArg: 2.093 ± 0.314
2.512GlnSer: 2.512 ± 0.432
2.177GlnThr: 2.177 ± 0.341
2.261GlnVal: 2.261 ± 0.385
0.502GlnTrp: 0.502 ± 0.204
2.093GlnTyr: 2.093 ± 0.339
0.0GlnXaa: 0.0 ± 0.0
Arg
2.344ArgAla: 2.344 ± 0.454
0.502ArgCys: 0.502 ± 0.201
3.517ArgAsp: 3.517 ± 0.465
3.6ArgGlu: 3.6 ± 0.561
2.344ArgPhe: 2.344 ± 0.417
3.098ArgGly: 3.098 ± 0.475
0.502ArgHis: 0.502 ± 0.172
3.266ArgIle: 3.266 ± 0.538
4.019ArgLys: 4.019 ± 0.541
4.605ArgLeu: 4.605 ± 0.568
1.507ArgMet: 1.507 ± 0.376
2.01ArgAsn: 2.01 ± 0.387
1.34ArgPro: 1.34 ± 0.318
2.512ArgGln: 2.512 ± 0.624
1.926ArgArg: 1.926 ± 0.386
1.591ArgSer: 1.591 ± 0.416
2.01ArgThr: 2.01 ± 0.489
3.098ArgVal: 3.098 ± 0.498
0.586ArgTrp: 0.586 ± 0.159
1.675ArgTyr: 1.675 ± 0.342
0.0ArgXaa: 0.0 ± 0.0
Ser
2.931SerAla: 2.931 ± 0.624
0.335SerCys: 0.335 ± 0.159
3.852SerAsp: 3.852 ± 0.457
4.438SerGlu: 4.438 ± 0.654
3.098SerPhe: 3.098 ± 0.628
3.266SerGly: 3.266 ± 0.514
1.172SerHis: 1.172 ± 0.362
5.024SerIle: 5.024 ± 0.67
4.438SerLys: 4.438 ± 0.628
4.354SerLeu: 4.354 ± 0.515
1.842SerMet: 1.842 ± 0.42
3.349SerAsn: 3.349 ± 0.57
1.926SerPro: 1.926 ± 0.468
1.842SerGln: 1.842 ± 0.336
2.344SerArg: 2.344 ± 0.38
3.266SerSer: 3.266 ± 0.455
2.931SerThr: 2.931 ± 0.568
4.103SerVal: 4.103 ± 0.666
0.251SerTrp: 0.251 ± 0.128
2.261SerTyr: 2.261 ± 0.349
0.0SerXaa: 0.0 ± 0.0
Thr
3.098ThrAla: 3.098 ± 0.612
0.335ThrCys: 0.335 ± 0.164
2.679ThrAsp: 2.679 ± 0.441
4.94ThrGlu: 4.94 ± 0.759
2.596ThrPhe: 2.596 ± 0.454
3.433ThrGly: 3.433 ± 0.592
0.921ThrHis: 0.921 ± 0.237
3.182ThrIle: 3.182 ± 0.584
5.861ThrLys: 5.861 ± 0.675
5.861ThrLeu: 5.861 ± 0.78
1.089ThrMet: 1.089 ± 0.251
2.763ThrAsn: 2.763 ± 0.647
1.591ThrPro: 1.591 ± 0.362
1.507ThrGln: 1.507 ± 0.314
1.758ThrArg: 1.758 ± 0.327
3.6ThrSer: 3.6 ± 0.375
4.354ThrThr: 4.354 ± 0.634
4.103ThrVal: 4.103 ± 0.392
0.754ThrTrp: 0.754 ± 0.207
1.842ThrTyr: 1.842 ± 0.327
0.0ThrXaa: 0.0 ± 0.0
Val
3.768ValAla: 3.768 ± 0.526
0.586ValCys: 0.586 ± 0.196
4.605ValAsp: 4.605 ± 0.605
5.191ValGlu: 5.191 ± 0.882
2.596ValPhe: 2.596 ± 0.459
3.852ValGly: 3.852 ± 0.507
0.586ValHis: 0.586 ± 0.249
3.517ValIle: 3.517 ± 0.528
4.438ValLys: 4.438 ± 0.579
4.354ValLeu: 4.354 ± 0.531
1.591ValMet: 1.591 ± 0.303
3.6ValAsn: 3.6 ± 0.497
2.01ValPro: 2.01 ± 0.358
3.182ValGln: 3.182 ± 0.415
2.093ValArg: 2.093 ± 0.366
4.354ValSer: 4.354 ± 0.461
3.349ValThr: 3.349 ± 0.542
4.103ValVal: 4.103 ± 0.721
1.005ValTrp: 1.005 ± 0.358
2.847ValTyr: 2.847 ± 0.563
0.0ValXaa: 0.0 ± 0.0
Trp
0.586TrpAla: 0.586 ± 0.18
0.167TrpCys: 0.167 ± 0.121
0.837TrpAsp: 0.837 ± 0.205
1.005TrpGlu: 1.005 ± 0.224
0.754TrpPhe: 0.754 ± 0.216
0.335TrpGly: 0.335 ± 0.182
0.167TrpHis: 0.167 ± 0.11
0.754TrpIle: 0.754 ± 0.251
1.172TrpLys: 1.172 ± 0.3
1.005TrpLeu: 1.005 ± 0.289
0.335TrpMet: 0.335 ± 0.2
0.921TrpAsn: 0.921 ± 0.264
0.084TrpPro: 0.084 ± 0.078
0.837TrpGln: 0.837 ± 0.255
0.586TrpArg: 0.586 ± 0.213
0.67TrpSer: 0.67 ± 0.177
0.586TrpThr: 0.586 ± 0.219
1.005TrpVal: 1.005 ± 0.362
0.084TrpTrp: 0.084 ± 0.076
0.335TrpTyr: 0.335 ± 0.129
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.093TyrAla: 2.093 ± 0.52
0.67TyrCys: 0.67 ± 0.219
1.423TyrAsp: 1.423 ± 0.288
2.679TyrGlu: 2.679 ± 0.458
2.512TyrPhe: 2.512 ± 0.432
1.842TyrGly: 1.842 ± 0.352
1.005TyrHis: 1.005 ± 0.294
2.931TyrIle: 2.931 ± 0.484
2.428TyrLys: 2.428 ± 0.483
3.517TyrLeu: 3.517 ± 0.738
1.005TyrMet: 1.005 ± 0.268
2.344TyrAsn: 2.344 ± 0.459
1.005TyrPro: 1.005 ± 0.309
1.675TyrGln: 1.675 ± 0.362
2.093TyrArg: 2.093 ± 0.463
1.423TyrSer: 1.423 ± 0.353
1.842TyrThr: 1.842 ± 0.435
2.428TyrVal: 2.428 ± 0.41
0.502TyrTrp: 0.502 ± 0.17
1.089TyrTyr: 1.089 ± 0.348
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (11944 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski