Candidatus Gracilibacteria bacterium GN02-872

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Patescibacteria group; Candidatus Gracilibacteria; unclassified Candidatus Gracilibacteria

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 972 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328EDI1|A0A328EDI1_9BACT Uncharacterized protein OS=Candidatus Gracilibacteria bacterium GN02-872 OX=1912928 GN=BLD25_03380 PE=4 SV=1
MM1 pKa = 7.19ITIKK5 pKa = 10.83DD6 pKa = 3.53SLGNVIKK13 pKa = 10.21EE14 pKa = 3.88IPADD18 pKa = 3.42INKK21 pKa = 8.73TLLEE25 pKa = 4.06QLTEE29 pKa = 4.04AGIDD33 pKa = 3.47IPAACYY39 pKa = 9.79TGVCGACMCEE49 pKa = 3.89IEE51 pKa = 5.22KK52 pKa = 11.06GSDD55 pKa = 3.4KK56 pKa = 10.72INKK59 pKa = 8.87NFTGEE64 pKa = 4.0EE65 pKa = 4.33GFPTDD70 pKa = 3.27EE71 pKa = 4.57TEE73 pKa = 4.76VMTCIAGITDD83 pKa = 4.03PDD85 pKa = 3.64AEE87 pKa = 5.5IILKK91 pKa = 9.01TIYY94 pKa = 10.71

Molecular weight: 10.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328EJ61|A0A328EJ61_9BACT Uncharacterized protein OS=Candidatus Gracilibacteria bacterium GN02-872 OX=1912928 GN=BLD25_00390 PE=4 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84KK4 pKa = 9.69AKK6 pKa = 9.6IAKK9 pKa = 9.57ANKK12 pKa = 8.53LKK14 pKa = 10.81KK15 pKa = 10.2RR16 pKa = 11.84FLQAIKK22 pKa = 10.54DD23 pKa = 3.59GRR25 pKa = 11.84KK26 pKa = 8.97PKK28 pKa = 10.54NATKK32 pKa = 10.54VFNCCSICGRR42 pKa = 11.84TRR44 pKa = 11.84GYY46 pKa = 9.91LGKK49 pKa = 10.81YY50 pKa = 7.95GICRR54 pKa = 11.84ICFRR58 pKa = 11.84EE59 pKa = 3.96KK60 pKa = 11.02ANAGEE65 pKa = 4.1LPGVRR70 pKa = 11.84KK71 pKa = 10.36SSWW74 pKa = 2.83

Molecular weight: 8.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

972

0

972

307244

59

4169

316.1

36.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.336 ± 0.07

0.684 ± 0.021

5.486 ± 0.055

8.221 ± 0.089

6.259 ± 0.11

6.323 ± 0.074

1.042 ± 0.026

9.813 ± 0.096

11.13 ± 0.099

9.17 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.808 ± 0.03

6.75 ± 0.088

2.46 ± 0.045

2.473 ± 0.046

2.925 ± 0.046

6.502 ± 0.069

4.666 ± 0.061

5.277 ± 0.058

0.59 ± 0.019

4.085 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski