Mycobacterium phage Curiosium

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6U0A3|A0A5J6U0A3_9CAUD Uncharacterized protein OS=Mycobacterium phage Curiosium OX=2599859 GN=79 PE=4 SV=1
MM1 pKa = 7.28SRR3 pKa = 11.84HH4 pKa = 5.55YY5 pKa = 10.17CTGDD9 pKa = 3.82DD10 pKa = 4.15CWHH13 pKa = 6.05CQPRR17 pKa = 11.84IDD19 pKa = 3.62QAEE22 pKa = 4.17YY23 pKa = 10.87EE24 pKa = 4.19RR25 pKa = 11.84DD26 pKa = 3.66CYY28 pKa = 11.44GDD30 pKa = 4.92DD31 pKa = 5.57DD32 pKa = 4.77YY33 pKa = 11.95PDD35 pKa = 4.53YY36 pKa = 11.71YY37 pKa = 11.27DD38 pKa = 3.5GTT40 pKa = 4.14

Molecular weight: 4.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TUM7|A0A5J6TUM7_9CAUD Uncharacterized protein OS=Mycobacterium phage Curiosium OX=2599859 GN=2 PE=4 SV=1
MM1 pKa = 7.37ACVTFRR7 pKa = 11.84LPSATAQLYY16 pKa = 9.66PNGPGAPKK24 pKa = 9.49WGVGAVPIPGVDD36 pKa = 3.15QGRR39 pKa = 11.84AASYY43 pKa = 9.78PRR45 pKa = 11.84APVNVSPVAVIPTVAPMRR63 pKa = 11.84LCAIRR68 pKa = 11.84CSPLALAGHH77 pKa = 6.7HH78 pKa = 6.58ASTGRR83 pKa = 11.84SAALLWSTRR92 pKa = 11.84SGDD95 pKa = 3.35GHH97 pKa = 7.41LLTDD101 pKa = 3.26QAPRR105 pKa = 11.84EE106 pKa = 3.99RR107 pKa = 11.84RR108 pKa = 11.84TRR110 pKa = 11.84MTEE113 pKa = 3.53QAPATEE119 pKa = 5.11APTEE123 pKa = 4.23TPAMDD128 pKa = 4.09PAARR132 pKa = 11.84NAALQAISMGDD143 pKa = 3.4KK144 pKa = 10.34GAHH147 pKa = 5.6TPTVYY152 pKa = 10.58TPMNAAEE159 pKa = 4.59AARR162 pKa = 11.84KK163 pKa = 8.03PRR165 pKa = 11.84RR166 pKa = 11.84RR167 pKa = 11.84PTHH170 pKa = 5.83KK171 pKa = 8.98ATRR174 pKa = 11.84PVVYY178 pKa = 9.85EE179 pKa = 4.44PYY181 pKa = 10.23SEE183 pKa = 4.04QQ184 pKa = 3.54

Molecular weight: 19.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

19399

40

1375

188.3

20.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.609 ± 0.482

1.211 ± 0.145

6.609 ± 0.319

5.773 ± 0.327

2.552 ± 0.167

8.985 ± 0.418

2.134 ± 0.178

3.882 ± 0.166

2.99 ± 0.18

8.33 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.104

2.67 ± 0.193

5.536 ± 0.222

3.428 ± 0.151

7.387 ± 0.371

4.892 ± 0.224

6.021 ± 0.198

8.191 ± 0.261

2.093 ± 0.108

2.376 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski