Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2752 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q0VTK1|Q0VTK1_ALCBS Uncharacterized protein OS=Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2) OX=393595 GN=ABO_0094 PE=4 SV=1
MM1 pKa = 7.42 NNFTRR6 pKa = 11.84 IISIAAISGISSIAIANTGEE26 pKa = 4.19 VIFNGVVSDD35 pKa = 4.03 TTCTLDD41 pKa = 3.81 VSQQGIAQADD51 pKa = 3.69 NTVTLDD57 pKa = 4.4 PITTAEE63 pKa = 4.23 FLASDD68 pKa = 4.14 TAYY71 pKa = 10.13 KK72 pKa = 9.67 ATGFSLVATDD82 pKa = 5.56 AGSATCNLTGKK93 pKa = 9.62 GASVQWTGQLYY104 pKa = 9.8 VGHH107 pKa = 7.11 NILQNIATGGAANVGMQLLEE127 pKa = 4.32 SDD129 pKa = 3.58 GTTPVTVNGSPSIIADD145 pKa = 3.38 ASGASLDD152 pKa = 3.96 FNVGYY157 pKa = 10.46 AATDD161 pKa = 3.34 ATAVTEE167 pKa = 4.21 GQVRR171 pKa = 11.84 SIANYY176 pKa = 10.31 SIVFNN181 pKa = 4.57
Molecular weight: 18.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.808
IPC_protein 3.757
Toseland 3.541
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.465
Solomon 3.757
Lehninger 3.706
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.897
Patrickios 0.769
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.787
Protein with the highest isoelectric point:
>sp|Q0VKY4|UREF_ALCBS Urease accessory protein UreF OS=Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2) OX=393595 GN=ureF PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.04 RR14 pKa = 11.84 AHH16 pKa = 6.12 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.27 NGRR28 pKa = 11.84 KK29 pKa = 8.98 ILAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.95 GRR39 pKa = 11.84 KK40 pKa = 8.8 RR41 pKa = 11.84 LAAA44 pKa = 4.42
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2752
0
2752
907762
33
3600
329.9
36.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.054 ± 0.057
0.996 ± 0.017
5.821 ± 0.036
6.047 ± 0.044
3.64 ± 0.032
7.782 ± 0.04
2.343 ± 0.027
4.945 ± 0.033
3.834 ± 0.039
11.093 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.644 ± 0.022
3.346 ± 0.027
4.703 ± 0.028
4.542 ± 0.036
6.168 ± 0.041
5.744 ± 0.033
5.068 ± 0.029
7.221 ± 0.041
1.452 ± 0.019
2.557 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here