Aggregatibacter segnis ATCC 33393

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Aggregatibacter; Aggregatibacter segnis

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1956 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6KWX3|E6KWX3_9PAST Protein of hypothetical function DUF1508 OS=Aggregatibacter segnis ATCC 33393 OX=888057 GN=HMPREF9064_0667 PE=3 SV=1
MM1 pKa = 7.33AVVGLFYY8 pKa = 11.0GSDD11 pKa = 3.2TGNTEE16 pKa = 4.47NIAKK20 pKa = 9.24MIQKK24 pKa = 9.54QLGSEE29 pKa = 4.08LVDD32 pKa = 3.05IRR34 pKa = 11.84DD35 pKa = 3.37IAKK38 pKa = 10.28SSKK41 pKa = 10.35EE42 pKa = 3.95DD43 pKa = 3.06IEE45 pKa = 6.2AYY47 pKa = 10.41DD48 pKa = 3.8FLLFGIPTWYY58 pKa = 10.39YY59 pKa = 11.58GEE61 pKa = 4.69AQCDD65 pKa = 3.26WDD67 pKa = 5.24DD68 pKa = 4.13FFPTLEE74 pKa = 4.64QIDD77 pKa = 4.09FTDD80 pKa = 3.2KK81 pKa = 10.68LVAIFGCGDD90 pKa = 3.19QEE92 pKa = 5.04DD93 pKa = 4.44YY94 pKa = 11.9ADD96 pKa = 4.07YY97 pKa = 10.93FCDD100 pKa = 3.82AMGTIRR106 pKa = 11.84NIVEE110 pKa = 3.6PHH112 pKa = 5.42GAIIVGHH119 pKa = 6.86WPTEE123 pKa = 3.93GYY125 pKa = 8.74TFEE128 pKa = 4.54SSQALVDD135 pKa = 4.33DD136 pKa = 3.93NTFVGLCIDD145 pKa = 4.19EE146 pKa = 4.82DD147 pKa = 3.95RR148 pKa = 11.84QPEE151 pKa = 4.34LTSEE155 pKa = 4.4RR156 pKa = 11.84VNKK159 pKa = 8.71WVKK162 pKa = 9.57QVYY165 pKa = 10.64DD166 pKa = 4.08EE167 pKa = 4.4MCLAEE172 pKa = 4.39LAA174 pKa = 4.24

Molecular weight: 19.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6KZD6|E6KZD6_9PAST Uncharacterized protein OS=Aggregatibacter segnis ATCC 33393 OX=888057 GN=HMPREF9064_1478 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84SRR14 pKa = 11.84THH16 pKa = 6.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1956

0

1956

601326

37

2245

307.4

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.578 ± 0.061

1.022 ± 0.019

5.03 ± 0.042

6.29 ± 0.058

4.445 ± 0.045

6.677 ± 0.059

2.19 ± 0.028

6.643 ± 0.053

6.114 ± 0.057

10.64 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.417 ± 0.026

4.745 ± 0.049

3.814 ± 0.036

4.943 ± 0.052

4.53 ± 0.043

5.652 ± 0.043

5.197 ± 0.037

6.72 ± 0.049

1.184 ± 0.023

3.168 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski