Palaemonetes sp. common grass shrimp associated circular virus
Average proteome isoelectric point is 8.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1RL70|A0A0K1RL70_9CIRC Putative capsid protein OS=Palaemonetes sp. common grass shrimp associated circular virus OX=1692259 PE=4 SV=1
MM1 pKa = 7.33 AAKK4 pKa = 9.89 RR5 pKa = 11.84 DD6 pKa = 3.86 SAVKK10 pKa = 10.14 RR11 pKa = 11.84 WCFTLNNYY19 pKa = 8.28 TDD21 pKa = 4.45 ADD23 pKa = 4.03 CEE25 pKa = 4.08 ALKK28 pKa = 10.87 EE29 pKa = 4.05 KK30 pKa = 9.96 LTTDD34 pKa = 3.18 TCSRR38 pKa = 11.84 AIVGKK43 pKa = 10.32 EE44 pKa = 3.38 KK45 pKa = 11.23 GEE47 pKa = 4.05 NGTPHH52 pKa = 6.39 LQGFVSLKK60 pKa = 7.08 TRR62 pKa = 11.84 KK63 pKa = 9.48 RR64 pKa = 11.84 LSAMKK69 pKa = 9.67 TFLSPRR75 pKa = 11.84 YY76 pKa = 9.46 HH77 pKa = 6.8 FEE79 pKa = 3.75 QAKK82 pKa = 8.4 GTDD85 pKa = 3.66 EE86 pKa = 4.65 QNTEE90 pKa = 4.11 YY91 pKa = 10.5 CSKK94 pKa = 10.73 EE95 pKa = 3.63 GDD97 pKa = 3.64 VLIDD101 pKa = 2.96 VGEE104 pKa = 4.16 NVKK107 pKa = 10.47 GRR109 pKa = 11.84 GDD111 pKa = 3.11 KK112 pKa = 11.26 GGGFNEE118 pKa = 4.3 GANIKK123 pKa = 10.2 RR124 pKa = 11.84 VVYY127 pKa = 10.31 HH128 pKa = 6.54 LLEE131 pKa = 4.98 GKK133 pKa = 10.08 DD134 pKa = 4.06 KK135 pKa = 11.01 EE136 pKa = 5.41 ALLQDD141 pKa = 3.74 EE142 pKa = 4.97 KK143 pKa = 11.32 MLGAYY148 pKa = 7.45 MKK150 pKa = 9.3 YY151 pKa = 9.92 KK152 pKa = 10.52 RR153 pKa = 11.84 SITEE157 pKa = 3.62 MVSEE161 pKa = 4.38 VKK163 pKa = 10.4 SEE165 pKa = 4.06 QEE167 pKa = 4.06 TKK169 pKa = 10.44 RR170 pKa = 11.84 LKK172 pKa = 10.31 QDD174 pKa = 3.37 LDD176 pKa = 3.69 NATLRR181 pKa = 11.84 PWQRR185 pKa = 11.84 EE186 pKa = 3.8 LKK188 pKa = 10.05 EE189 pKa = 4.05 YY190 pKa = 9.91 LTGDD194 pKa = 3.09 PHH196 pKa = 6.94 DD197 pKa = 4.34 RR198 pKa = 11.84 HH199 pKa = 7.2 IIWYY203 pKa = 9.54 IDD205 pKa = 3.35 GQGNNGKK212 pKa = 9.11 SWFAKK217 pKa = 9.65 YY218 pKa = 9.85 CVAEE222 pKa = 4.37 LGAIRR227 pKa = 11.84 LEE229 pKa = 4.2 NAKK232 pKa = 8.62 TADD235 pKa = 3.42 LTHH238 pKa = 7.17 AYY240 pKa = 9.89 NGEE243 pKa = 3.94 RR244 pKa = 11.84 VVIFDD249 pKa = 4.88 LSRR252 pKa = 11.84 TTEE255 pKa = 3.75 GHH257 pKa = 5.82 FNYY260 pKa = 10.36 GALEE264 pKa = 4.28 SIKK267 pKa = 10.36 NGCVFSSKK275 pKa = 10.46 YY276 pKa = 10.42 DD277 pKa = 3.4 SRR279 pKa = 11.84 QKK281 pKa = 9.99 MFPIPHH287 pKa = 6.29 VIVFANWGPDD297 pKa = 3.06 RR298 pKa = 11.84 SKK300 pKa = 10.92 LSEE303 pKa = 4.33 DD304 pKa = 2.84 RR305 pKa = 11.84 WVTKK309 pKa = 8.48 TWSFEE314 pKa = 3.86 QEE316 pKa = 4.04 TFQTFF321 pKa = 2.92
Molecular weight: 36.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.036
IPC2_protein 7.468
IPC_protein 7.395
Toseland 7.161
ProMoST 7.892
Dawson 8.112
Bjellqvist 8.361
Wikipedia 8.039
Rodwell 8.126
Grimsley 7.22
Solomon 8.214
Lehninger 8.229
Nozaki 8.565
DTASelect 8.17
Thurlkill 8.244
EMBOSS 8.346
Sillero 8.536
Patrickios 4.546
IPC_peptide 8.214
IPC2_peptide 7.293
IPC2.peptide.svr19 7.528
Protein with the highest isoelectric point:
>tr|A0A0K1RL70|A0A0K1RL70_9CIRC Putative capsid protein OS=Palaemonetes sp. common grass shrimp associated circular virus OX=1692259 PE=4 SV=1
MM1 pKa = 7.49 LPFAVEE7 pKa = 4.43 LAQNAYY13 pKa = 10.06 AAYY16 pKa = 9.4 EE17 pKa = 4.04 LGKK20 pKa = 10.3 QGYY23 pKa = 8.85 EE24 pKa = 3.99 YY25 pKa = 11.08 YY26 pKa = 10.43 KK27 pKa = 10.58 DD28 pKa = 3.61 YY29 pKa = 11.38 SPYY32 pKa = 9.98 VYY34 pKa = 10.29 RR35 pKa = 11.84 AGRR38 pKa = 11.84 GVKK41 pKa = 9.79 RR42 pKa = 11.84 KK43 pKa = 10.17 LGAFLQNEE51 pKa = 4.21 QDD53 pKa = 4.33 LDD55 pKa = 3.83 DD56 pKa = 4.58 SPAMRR61 pKa = 11.84 RR62 pKa = 11.84 MRR64 pKa = 11.84 RR65 pKa = 11.84 YY66 pKa = 9.46 SRR68 pKa = 11.84 RR69 pKa = 11.84 GMLMRR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 FRR78 pKa = 11.84 RR79 pKa = 11.84 RR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 11.84 VFRR85 pKa = 11.84 RR86 pKa = 11.84 SRR88 pKa = 11.84 RR89 pKa = 11.84 VFRR92 pKa = 11.84 RR93 pKa = 11.84 RR94 pKa = 11.84 RR95 pKa = 11.84 AFRR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 SGVITSLLRR109 pKa = 11.84 FEE111 pKa = 4.81 TDD113 pKa = 2.8 IKK115 pKa = 11.0 LDD117 pKa = 3.88 SNGVANNSLYY127 pKa = 11.1 FHH129 pKa = 7.04 MGTLRR134 pKa = 11.84 TKK136 pKa = 10.25 INSAYY141 pKa = 9.77 IHH143 pKa = 6.33 MFDD146 pKa = 3.63 EE147 pKa = 5.27 FKK149 pKa = 10.62 ILSISVVMRR158 pKa = 11.84 HH159 pKa = 6.41 DD160 pKa = 4.08 KK161 pKa = 11.26 NMSATDD167 pKa = 3.6 NAITEE172 pKa = 4.4 LWWTYY177 pKa = 11.61 DD178 pKa = 3.23 PDD180 pKa = 3.56 LTGRR184 pKa = 11.84 KK185 pKa = 8.58 IDD187 pKa = 3.51 MDD189 pKa = 4.3 NIKK192 pKa = 9.25 MVRR195 pKa = 11.84 RR196 pKa = 11.84 ARR198 pKa = 11.84 SVHH201 pKa = 6.31 LGPMGKK207 pKa = 9.38 KK208 pKa = 9.51 VLKK211 pKa = 10.0 CYY213 pKa = 10.16 PIFVNPTKK221 pKa = 10.99 LEE223 pKa = 4.19 GGEE226 pKa = 3.98 QQQTISSRR234 pKa = 11.84 GSAYY238 pKa = 10.09 NRR240 pKa = 11.84 PWFDD244 pKa = 3.12 AAGLNVGYY252 pKa = 10.32 SDD254 pKa = 5.09 AMKK257 pKa = 10.23 SHH259 pKa = 6.75 NGIAWALEE267 pKa = 3.99 GQPGRR272 pKa = 11.84 GLRR275 pKa = 11.84 LDD277 pKa = 3.55 YY278 pKa = 10.6 AIRR281 pKa = 11.84 IAWRR285 pKa = 11.84 GRR287 pKa = 11.84 RR288 pKa = 11.84 DD289 pKa = 3.31 GQTYY293 pKa = 9.5 TGDD296 pKa = 3.33 SS297 pKa = 3.48
Molecular weight: 34.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.955
IPC_protein 10.891
Toseland 10.745
ProMoST 10.599
Dawson 10.877
Bjellqvist 10.657
Wikipedia 11.14
Rodwell 10.965
Grimsley 10.935
Solomon 11.023
Lehninger 10.965
Nozaki 10.716
DTASelect 10.657
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.804
Patrickios 10.613
IPC_peptide 11.023
IPC2_peptide 9.692
IPC2.peptide.svr19 8.296
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
618
297
321
309.0
35.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.796 ± 0.419
1.133 ± 0.546
6.149 ± 0.061
5.987 ± 1.798
4.207 ± 0.114
7.929 ± 0.104
2.104 ± 0.288
4.207 ± 0.348
7.443 ± 1.642
7.443 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.074 ± 0.894
4.531 ± 0.105
2.589 ± 0.303
3.236 ± 0.141
10.032 ± 3.051
5.987 ± 0.282
5.502 ± 1.003
5.016 ± 0.207
1.942 ± 0.177
4.693 ± 0.708
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here