Streptococcus phage CHPC577

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L2JXM1|A0A1L2JXM1_9CAUD Replication protein OS=Streptococcus phage CHPC577 OX=1913081 GN=CHPC577_0037 PE=4 SV=1
MM1 pKa = 7.11VNWVDD6 pKa = 4.59ADD8 pKa = 4.03GNDD11 pKa = 3.35ITDD14 pKa = 4.04GADD17 pKa = 3.2QEE19 pKa = 5.11FKK21 pKa = 11.28AGMFFSFAGDD31 pKa = 3.56EE32 pKa = 4.15NNITDD37 pKa = 3.82TGEE40 pKa = 3.65GGYY43 pKa = 10.61YY44 pKa = 9.76GGYY47 pKa = 8.0YY48 pKa = 9.41YY49 pKa = 10.76RR50 pKa = 11.84RR51 pKa = 11.84FEE53 pKa = 4.06FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L2JXL6|A0A1L2JXL6_9CAUD Lysin OS=Streptococcus phage CHPC577 OX=1913081 GN=CHPC577_0022 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 9.22TKK5 pKa = 10.5RR6 pKa = 11.84GVCANCHH13 pKa = 4.61TVFEE17 pKa = 4.53VSKK20 pKa = 10.01KK21 pKa = 6.75QRR23 pKa = 11.84RR24 pKa = 11.84KK25 pKa = 8.8IKK27 pKa = 8.88EE28 pKa = 4.09GKK30 pKa = 9.71SVFCSEE36 pKa = 4.16TCSLEE41 pKa = 4.41KK42 pKa = 10.69YY43 pKa = 9.93GKK45 pKa = 8.13TKK47 pKa = 9.25ITISEE52 pKa = 4.39IPCCRR57 pKa = 11.84CGKK60 pKa = 9.2MFTPTYY66 pKa = 9.91SQYY69 pKa = 11.19KK70 pKa = 7.81RR71 pKa = 11.84YY72 pKa = 9.99KK73 pKa = 8.85YY74 pKa = 10.7NDD76 pKa = 3.28YY77 pKa = 10.92VSNSFCSNEE86 pKa = 3.99CRR88 pKa = 11.84WKK90 pKa = 10.76KK91 pKa = 10.58EE92 pKa = 3.92YY93 pKa = 10.4LCKK96 pKa = 10.86YY97 pKa = 8.47HH98 pKa = 7.64DD99 pKa = 5.0DD100 pKa = 4.01YY101 pKa = 12.19VSVFVNGKK109 pKa = 9.91EE110 pKa = 3.7ILLDD114 pKa = 3.54FDD116 pKa = 4.36VFEE119 pKa = 5.64KK120 pKa = 9.7YY121 pKa = 8.83TKK123 pKa = 9.47TLYY126 pKa = 9.5VHH128 pKa = 6.45NDD130 pKa = 2.31KK131 pKa = 10.86RR132 pKa = 11.84SNYY135 pKa = 8.89NPVHH139 pKa = 6.14VFEE142 pKa = 5.31KK143 pKa = 10.71GKK145 pKa = 8.86KK146 pKa = 6.8TLSRR150 pKa = 11.84LIMSVTDD157 pKa = 3.33KK158 pKa = 11.24NKK160 pKa = 10.87YY161 pKa = 8.69IDD163 pKa = 4.86HH164 pKa = 7.04INGNPLDD171 pKa = 3.85NRR173 pKa = 11.84RR174 pKa = 11.84SNLRR178 pKa = 11.84VVSHH182 pKa = 5.96QEE184 pKa = 3.05NMMNKK189 pKa = 8.25KK190 pKa = 8.41TYY192 pKa = 10.6KK193 pKa = 10.75NNTSKK198 pKa = 10.74IKK200 pKa = 10.53GVNLNKK206 pKa = 10.19KK207 pKa = 8.12GLWVARR213 pKa = 11.84IQVRR217 pKa = 11.84NQRR220 pKa = 11.84IFLGSSKK227 pKa = 10.56DD228 pKa = 3.26KK229 pKa = 11.18SVAEE233 pKa = 4.0RR234 pKa = 11.84LRR236 pKa = 11.84IEE238 pKa = 4.16AEE240 pKa = 3.57KK241 pKa = 10.62KK242 pKa = 10.51YY243 pKa = 10.45FGKK246 pKa = 10.57YY247 pKa = 8.25DD248 pKa = 3.53RR249 pKa = 11.84KK250 pKa = 10.2YY251 pKa = 11.25LKK253 pKa = 10.64

Molecular weight: 29.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10472

38

916

209.4

23.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.197 ± 0.363

0.802 ± 0.134

5.987 ± 0.226

6.961 ± 0.483

4.45 ± 0.24

6.436 ± 0.415

1.117 ± 0.123

6.981 ± 0.376

8.833 ± 0.422

7.553 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.179

6.799 ± 0.396

2.807 ± 0.18

3.762 ± 0.237

4.24 ± 0.314

6.57 ± 0.356

6.236 ± 0.259

6.293 ± 0.298

1.604 ± 0.157

4.001 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski