Streptococcus satellite phage Javan152

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZF34|A0A4D5ZF34_9VIRU Integrase OS=Streptococcus satellite phage Javan152 OX=2558544 GN=JavanS152_0001 PE=3 SV=1
MM1 pKa = 7.93DD2 pKa = 5.55FSDD5 pKa = 5.66FLNKK9 pKa = 9.27KK10 pKa = 8.11QKK12 pKa = 10.01EE13 pKa = 4.11WDD15 pKa = 3.62EE16 pKa = 4.17SHH18 pKa = 7.64PIPDD22 pKa = 4.37FSAMSDD28 pKa = 3.39EE29 pKa = 4.1KK30 pKa = 11.24LLYY33 pKa = 10.5QLMSEE38 pKa = 4.38ALVSEE43 pKa = 4.67KK44 pKa = 10.12FAKK47 pKa = 10.04EE48 pKa = 3.65LSS50 pKa = 3.62

Molecular weight: 5.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZF44|A0A4D5ZF44_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan152 OX=2558544 GN=JavanS152_0011 PE=4 SV=1
MM1 pKa = 7.92KK2 pKa = 9.25ITEE5 pKa = 4.18YY6 pKa = 10.59KK7 pKa = 9.86KK8 pKa = 10.3KK9 pKa = 10.54NGTIVYY15 pKa = 9.09RR16 pKa = 11.84ANIYY20 pKa = 10.61LGIDD24 pKa = 3.32VVTGKK29 pKa = 10.22KK30 pKa = 10.2VKK32 pKa = 9.86TSITGRR38 pKa = 11.84TRR40 pKa = 11.84KK41 pKa = 8.72EE42 pKa = 3.89VKK44 pKa = 10.12QKK46 pKa = 9.84TKK48 pKa = 10.35EE49 pKa = 3.77AQQAFKK55 pKa = 11.32ANGQTVTEE63 pKa = 4.06ILSLKK68 pKa = 8.87TYY70 pKa = 10.82KK71 pKa = 10.45EE72 pKa = 3.98LANLWLEE79 pKa = 4.4SYY81 pKa = 10.77QLTVKK86 pKa = 9.72PQSFISAKK94 pKa = 10.49GILSNYY100 pKa = 8.81LLPAFGDD107 pKa = 3.65VKK109 pKa = 10.78LDD111 pKa = 3.72KK112 pKa = 11.09LSLPYY117 pKa = 9.81IQSVINNISSRR128 pKa = 11.84LVNYY132 pKa = 10.97AMVHH136 pKa = 6.12SINKK140 pKa = 9.95RR141 pKa = 11.84ILQYY145 pKa = 10.83GVSLQLIPTNPARR158 pKa = 11.84DD159 pKa = 3.57VILPKK164 pKa = 10.37VSRR167 pKa = 11.84EE168 pKa = 3.46EE169 pKa = 3.81SKK171 pKa = 10.9AIKK174 pKa = 10.35FIDD177 pKa = 3.85SNILKK182 pKa = 10.55ALMSYY187 pKa = 9.22MEE189 pKa = 4.35NVSNQDD195 pKa = 2.79YY196 pKa = 10.33RR197 pKa = 11.84YY198 pKa = 8.62YY199 pKa = 10.66FDD201 pKa = 3.66YY202 pKa = 11.62VMYY205 pKa = 10.67SLLLATGCRR214 pKa = 11.84FGEE217 pKa = 4.36ACALEE222 pKa = 4.16WSDD225 pKa = 5.9IDD227 pKa = 4.04FCSNTININKK237 pKa = 9.76SYY239 pKa = 10.16NRR241 pKa = 11.84NVRR244 pKa = 11.84LVGDD248 pKa = 4.31PKK250 pKa = 10.73SKK252 pKa = 10.85AGIRR256 pKa = 11.84VISVDD261 pKa = 3.2QKK263 pKa = 9.97TINLLRR269 pKa = 11.84LYY271 pKa = 10.67KK272 pKa = 10.3NRR274 pKa = 11.84QRR276 pKa = 11.84QLFAEE281 pKa = 4.51VGNGSPKK288 pKa = 10.36VVFSTPTKK296 pKa = 9.35EE297 pKa = 4.08YY298 pKa = 10.3QDD300 pKa = 2.71ISTRR304 pKa = 11.84QEE306 pKa = 3.84ALNKK310 pKa = 9.12YY311 pKa = 9.62LNAISCPRR319 pKa = 11.84FTFHH323 pKa = 7.96AFRR326 pKa = 11.84HH327 pKa = 4.69THH329 pKa = 6.92ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.52RR346 pKa = 11.84LGHH349 pKa = 6.62AKK351 pKa = 10.36LAMTMDD357 pKa = 4.64TYY359 pKa = 11.86SHH361 pKa = 7.07LSKK364 pKa = 10.81AKK366 pKa = 8.9EE367 pKa = 4.01KK368 pKa = 10.63EE369 pKa = 3.89AVSYY373 pKa = 9.52YY374 pKa = 10.59EE375 pKa = 4.03KK376 pKa = 10.7AINGLL381 pKa = 3.67

Molecular weight: 43.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2390

50

381

159.3

18.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.77 ± 0.578

0.962 ± 0.347

5.146 ± 0.547

10.293 ± 0.968

4.644 ± 0.597

3.138 ± 0.321

1.632 ± 0.276

7.782 ± 0.374

10.753 ± 0.415

10.586 ± 0.476

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.211

6.695 ± 0.656

2.134 ± 0.263

4.351 ± 0.469

4.142 ± 0.518

6.695 ± 0.481

4.937 ± 0.607

3.933 ± 0.554

0.879 ± 0.197

4.393 ± 0.444
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski