Nematocida parisii (strain ERTm3) (Nematode killer fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Microsporidia incertae sedis; Nematocida; Nematocida parisii

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2724 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3EG79|I3EG79_NEMP3 Uncharacterized protein OS=Nematocida parisii (strain ERTm3) OX=935791 GN=NEQG_01670 PE=4 SV=1
MM1 pKa = 7.6EE2 pKa = 5.43NFMSNYY8 pKa = 9.91DD9 pKa = 3.91EE10 pKa = 4.25QPNTIDD16 pKa = 3.26TPGFEE21 pKa = 4.68TEE23 pKa = 4.33TIVNNDD29 pKa = 3.76EE30 pKa = 3.81LTIIQNIEE38 pKa = 3.8QNVYY42 pKa = 10.54SVSLDD47 pKa = 3.47TAVEE51 pKa = 4.24CGVEE55 pKa = 4.11QIPVDD60 pKa = 3.86PKK62 pKa = 10.4IEE64 pKa = 3.91QLISAFASEE73 pKa = 4.32NRR75 pKa = 11.84FITTNYY81 pKa = 10.12SSNEE85 pKa = 3.54QLIRR89 pKa = 11.84DD90 pKa = 3.98VLSSLEE96 pKa = 4.06KK97 pKa = 10.59KK98 pKa = 10.43GVLFAEE104 pKa = 5.46DD105 pKa = 4.39DD106 pKa = 3.98LSTLSTYY113 pKa = 10.92LNEE116 pKa = 4.17YY117 pKa = 9.69GPLVTDD123 pKa = 3.61VSMSTVLNDD132 pKa = 3.49TNLVTEE138 pKa = 4.86VILTLSEE145 pKa = 3.8NAAEE149 pKa = 3.96ISEE152 pKa = 4.35LNGEE156 pKa = 4.09KK157 pKa = 10.39VLRR160 pKa = 11.84IVSSVDD166 pKa = 3.22LEE168 pKa = 4.29SAYY171 pKa = 11.1SYY173 pKa = 10.92MKK175 pKa = 10.77VCTANQAIVDD185 pKa = 3.87AQSNIFNDD193 pKa = 3.23ICSSLTINMPNLTEE207 pKa = 4.72KK208 pKa = 10.32IVEE211 pKa = 4.24LVEE214 pKa = 4.24TAAEE218 pKa = 3.9PAINAMNN225 pKa = 4.03

Molecular weight: 24.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3ED24|I3ED24_NEMP3 Uncharacterized protein OS=Nematocida parisii (strain ERTm3) OX=935791 GN=NEQG_02690 PE=4 SV=1
MM1 pKa = 7.49VNTQTAIQTAMKK13 pKa = 9.34TVTEE17 pKa = 4.34TATQAINFIRR27 pKa = 11.84EE28 pKa = 4.33SVSEE32 pKa = 4.0ISLDD36 pKa = 4.0GIKK39 pKa = 10.38DD40 pKa = 3.76IIYY43 pKa = 10.48ASTDD47 pKa = 3.22EE48 pKa = 5.29QIDD51 pKa = 3.6LRR53 pKa = 11.84NRR55 pKa = 11.84CILISIAAITLIIVTCVYY73 pKa = 10.44CIIRR77 pKa = 11.84NKK79 pKa = 10.32LKK81 pKa = 10.07AAKK84 pKa = 9.7ARR86 pKa = 11.84RR87 pKa = 11.84QAGQTEE93 pKa = 4.39AEE95 pKa = 4.06RR96 pKa = 11.84QEE98 pKa = 4.18EE99 pKa = 4.47KK100 pKa = 10.66EE101 pKa = 3.82KK102 pKa = 11.11SRR104 pKa = 11.84CKK106 pKa = 9.6KK107 pKa = 9.28TSTSKK112 pKa = 9.97CSRR115 pKa = 11.84YY116 pKa = 9.34NRR118 pKa = 11.84RR119 pKa = 11.84STYY122 pKa = 7.86TTDD125 pKa = 3.0INRR128 pKa = 11.84STSTGCQASTSRR140 pKa = 11.84RR141 pKa = 11.84RR142 pKa = 11.84KK143 pKa = 7.83NTSRR147 pKa = 11.84CKK149 pKa = 9.59KK150 pKa = 9.24TSTCKK155 pKa = 10.23CSRR158 pKa = 11.84KK159 pKa = 8.53KK160 pKa = 9.31TSRR163 pKa = 11.84CNKK166 pKa = 6.83KK167 pKa = 7.31TRR169 pKa = 11.84RR170 pKa = 11.84RR171 pKa = 11.84KK172 pKa = 9.8KK173 pKa = 7.99NTSRR177 pKa = 11.84RR178 pKa = 11.84SKK180 pKa = 10.68KK181 pKa = 8.37KK182 pKa = 8.36TRR184 pKa = 11.84RR185 pKa = 11.84CRR187 pKa = 3.3

Molecular weight: 21.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2724

0

2724

955631

41

3274

350.8

40.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.85 ± 0.047

1.772 ± 0.021

4.74 ± 0.032

7.532 ± 0.059

4.036 ± 0.035

4.393 ± 0.042

2.042 ± 0.02

9.315 ± 0.052

8.384 ± 0.048

9.182 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.605 ± 0.02

5.952 ± 0.058

3.375 ± 0.037

3.066 ± 0.028

4.214 ± 0.033

8.238 ± 0.059

5.675 ± 0.032

5.641 ± 0.036

0.665 ± 0.01

4.323 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski