Maize streak virus genotype C (isolate Set) (MSV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus; Maize streak virus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O40987|REPA_MSVSE Replication-associated protein A OS=Maize streak virus genotype C (isolate Set) OX=268344 GN=C1 PE=3 SV=1
MM1 pKa = 7.36TSSSSNRR8 pKa = 11.84PFSHH12 pKa = 7.21RR13 pKa = 11.84SPNTFLTYY21 pKa = 8.92PQCPEE26 pKa = 3.58QPEE29 pKa = 4.67IISQRR34 pKa = 11.84IWDD37 pKa = 4.54LCSHH41 pKa = 6.45WTPLYY46 pKa = 9.99IICARR51 pKa = 11.84EE52 pKa = 3.66AHH54 pKa = 7.0RR55 pKa = 11.84DD56 pKa = 3.48GNQCLHH62 pKa = 6.96ALIQTEE68 pKa = 4.39KK69 pKa = 10.47PVRR72 pKa = 11.84TTDD75 pKa = 3.35SRR77 pKa = 11.84FFDD80 pKa = 3.22IDD82 pKa = 3.48GFHH85 pKa = 7.33PNIQSAISPNKK96 pKa = 8.69VRR98 pKa = 11.84DD99 pKa = 4.03YY100 pKa = 10.01ITKK103 pKa = 10.03EE104 pKa = 3.48PLALFEE110 pKa = 5.16RR111 pKa = 11.84GTFIPRR117 pKa = 11.84KK118 pKa = 9.71KK119 pKa = 10.54SFLGNSSKK127 pKa = 11.17GNSDD131 pKa = 3.42KK132 pKa = 11.17KK133 pKa = 10.07PSKK136 pKa = 10.64DD137 pKa = 3.17EE138 pKa = 3.66IMRR141 pKa = 11.84DD142 pKa = 3.68IISHH146 pKa = 5.65ATSKK150 pKa = 10.77QEE152 pKa = 3.99YY153 pKa = 9.69LSMVQKK159 pKa = 10.52SLPYY163 pKa = 10.04DD164 pKa = 3.27WSTKK168 pKa = 8.97LQYY171 pKa = 10.45FEE173 pKa = 4.94YY174 pKa = 10.26SANKK178 pKa = 9.66LFPDD182 pKa = 3.26IQEE185 pKa = 4.21EE186 pKa = 4.74FINPHH191 pKa = 6.03PTSEE195 pKa = 4.43PDD197 pKa = 3.53LLCNEE202 pKa = 5.12SIKK205 pKa = 11.07DD206 pKa = 3.33WLQPNIYY213 pKa = 9.48QVSPQAYY220 pKa = 10.21LLLQPNCYY228 pKa = 10.43SIDD231 pKa = 3.73DD232 pKa = 5.18AISDD236 pKa = 5.02LEE238 pKa = 4.18WLDD241 pKa = 4.88DD242 pKa = 3.91LTSKK246 pKa = 10.89QIMEE250 pKa = 4.24QGSAASTSSVQQGQEE265 pKa = 3.86NLHH268 pKa = 6.08GPEE271 pKa = 3.88AA272 pKa = 4.43

Molecular weight: 31.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O40986|REP_MSVSE Replication-associated protein OS=Maize streak virus genotype C (isolate Set) OX=268344 GN=C1/C2 PE=3 SV=2
MM1 pKa = 6.95STSKK5 pKa = 10.63RR6 pKa = 11.84KK7 pKa = 9.69RR8 pKa = 11.84ADD10 pKa = 3.09EE11 pKa = 4.2AQWNKK16 pKa = 10.41RR17 pKa = 11.84STKK20 pKa = 10.13KK21 pKa = 10.39KK22 pKa = 10.36GSAPQAKK29 pKa = 9.74KK30 pKa = 10.39PGGKK34 pKa = 8.73VEE36 pKa = 4.34KK37 pKa = 9.84PSLQIQTLLHH47 pKa = 6.55SGDD50 pKa = 3.49TMITVPSGGVCDD62 pKa = 6.17LINTYY67 pKa = 11.02ARR69 pKa = 11.84GSDD72 pKa = 3.04EE73 pKa = 4.39GNRR76 pKa = 11.84HH77 pKa = 4.54TSEE80 pKa = 3.79TLTYY84 pKa = 10.21KK85 pKa = 10.8VGVDD89 pKa = 3.04YY90 pKa = 11.43HH91 pKa = 6.46FVADD95 pKa = 4.09AASCKK100 pKa = 9.91YY101 pKa = 10.2SNRR104 pKa = 11.84GTGVMWLVYY113 pKa = 9.39DD114 pKa = 4.05TTPGGNAPTTQDD126 pKa = 2.42IFAYY130 pKa = 9.83PSALKK135 pKa = 10.25AWPTTWKK142 pKa = 10.31VSRR145 pKa = 11.84EE146 pKa = 3.85LCHH149 pKa = 6.49RR150 pKa = 11.84FVVKK154 pKa = 10.41RR155 pKa = 11.84RR156 pKa = 11.84WLFTMEE162 pKa = 3.72TDD164 pKa = 3.84GRR166 pKa = 11.84IGSDD170 pKa = 3.47TPPSNQSWPPCKK182 pKa = 10.15RR183 pKa = 11.84NVDD186 pKa = 4.1FHH188 pKa = 7.43KK189 pKa = 9.79FTSGLGVRR197 pKa = 11.84TQWKK201 pKa = 8.76NVTDD205 pKa = 4.02GGVGAIQRR213 pKa = 11.84GALYY217 pKa = 10.5LVIAPGNGITFTAHH231 pKa = 5.19GQTRR235 pKa = 11.84LYY237 pKa = 9.73FKK239 pKa = 10.92SVGNQQ244 pKa = 2.8

Molecular weight: 26.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

971

101

354

242.8

27.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.84 ± 0.856

2.06 ± 0.241

5.458 ± 0.379

5.252 ± 0.883

3.708 ± 0.175

5.561 ± 1.522

2.575 ± 0.166

6.282 ± 0.901

6.488 ± 0.96

7.003 ± 0.894

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.751 ± 0.231

4.428 ± 0.524

7.621 ± 0.727

5.046 ± 0.567

5.046 ± 0.443

9.063 ± 0.794

6.797 ± 1.056

4.531 ± 1.081

2.472 ± 0.245

4.016 ± 0.474
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski