Pteropus associated gemycircularvirus 8

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus ptero8

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTQ2|A0A140CTQ2_9VIRU RepA OS=Pteropus associated gemycircularvirus 8 OX=1985402 PE=3 SV=1
MM1 pKa = 7.72APFILKK7 pKa = 9.72NVRR10 pKa = 11.84RR11 pKa = 11.84ALLTYY16 pKa = 7.65SQCGTLDD23 pKa = 3.44PFAVSDD29 pKa = 3.63HH30 pKa = 6.2FSNLGAEE37 pKa = 4.53CIVGRR42 pKa = 11.84EE43 pKa = 3.75RR44 pKa = 11.84HH45 pKa = 5.94ADD47 pKa = 3.43GGLHH51 pKa = 5.93LHH53 pKa = 6.03VFVDD57 pKa = 4.61FGRR60 pKa = 11.84QFSSRR65 pKa = 11.84KK66 pKa = 7.81TDD68 pKa = 3.11VFDD71 pKa = 4.59VGGHH75 pKa = 6.1HH76 pKa = 7.25PNIAKK81 pKa = 10.36CGRR84 pKa = 11.84TPWKK88 pKa = 10.45AYY90 pKa = 10.52DD91 pKa = 3.69YY92 pKa = 10.6AIKK95 pKa = 10.92DD96 pKa = 3.52GDD98 pKa = 4.09VVAGGLDD105 pKa = 3.48RR106 pKa = 11.84PIEE109 pKa = 4.01EE110 pKa = 4.85SGDD113 pKa = 3.54GSGTIDD119 pKa = 3.9STWHH123 pKa = 6.33SILAAEE129 pKa = 4.46TPEE132 pKa = 4.2EE133 pKa = 4.41FWALCHH139 pKa = 7.01DD140 pKa = 4.97LAPRR144 pKa = 11.84DD145 pKa = 3.72LARR148 pKa = 11.84SFPSLQKK155 pKa = 10.22YY156 pKa = 9.88CDD158 pKa = 2.84WRR160 pKa = 11.84YY161 pKa = 10.12RR162 pKa = 11.84PVTRR166 pKa = 11.84PYY168 pKa = 8.77CTPDD172 pKa = 3.0GYY174 pKa = 11.41EE175 pKa = 4.13FDD177 pKa = 3.55TSRR180 pKa = 11.84APEE183 pKa = 4.1LNEE186 pKa = 3.56WLLQANLGLRR196 pKa = 11.84GNWDD200 pKa = 2.89QGMCC204 pKa = 4.51

Molecular weight: 22.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTQ1|A0A140CTQ1_9VIRU Replication-associated protein OS=Pteropus associated gemycircularvirus 8 OX=1985402 PE=3 SV=1
MM1 pKa = 7.48PRR3 pKa = 11.84YY4 pKa = 7.13ATRR7 pKa = 11.84RR8 pKa = 11.84ISGRR12 pKa = 11.84YY13 pKa = 9.36SKK15 pKa = 9.62TKK17 pKa = 8.85QANRR21 pKa = 11.84YY22 pKa = 6.18KK23 pKa = 10.74AKK25 pKa = 10.62YY26 pKa = 7.84KK27 pKa = 10.37RR28 pKa = 11.84KK29 pKa = 8.77YY30 pKa = 8.04KK31 pKa = 10.68AKK33 pKa = 9.6RR34 pKa = 11.84KK35 pKa = 8.95YY36 pKa = 11.0GGMSKK41 pKa = 10.56KK42 pKa = 10.48KK43 pKa = 10.28ILDD46 pKa = 3.1LTAIKK51 pKa = 10.42KK52 pKa = 9.71RR53 pKa = 11.84DD54 pKa = 3.51AMLVSTNITLASPSGSTSFNNNGAVLGPNRR84 pKa = 11.84TYY86 pKa = 10.53FIPWIATWRR95 pKa = 11.84DD96 pKa = 2.86NSTVSGGAQTGTRR109 pKa = 11.84FDD111 pKa = 3.59TATRR115 pKa = 11.84TATTCYY121 pKa = 9.61MRR123 pKa = 11.84GLKK126 pKa = 9.11EE127 pKa = 3.71QIYY130 pKa = 9.44FIQNDD135 pKa = 3.66GSQWMWRR142 pKa = 11.84RR143 pKa = 11.84ICFKK147 pKa = 10.04YY148 pKa = 10.16RR149 pKa = 11.84GPVVYY154 pKa = 9.86QQSKK158 pKa = 10.15AGYY161 pKa = 7.42TLAVEE166 pKa = 4.66TSNGFQRR173 pKa = 11.84LYY175 pKa = 11.62NNINDD180 pKa = 3.75SSGANMLNTMIGIMFRR196 pKa = 11.84GQVGVDD202 pKa = 2.95YY203 pKa = 11.39GNFFNAPTDD212 pKa = 3.49NTRR215 pKa = 11.84CEE217 pKa = 3.68IVYY220 pKa = 10.73DD221 pKa = 3.84KK222 pKa = 10.86TRR224 pKa = 11.84SLATGNQNGYY234 pKa = 7.54SRR236 pKa = 11.84RR237 pKa = 11.84VSLWHH242 pKa = 6.47GMNKK246 pKa = 9.65NLVYY250 pKa = 10.85DD251 pKa = 4.21DD252 pKa = 5.5DD253 pKa = 4.32EE254 pKa = 6.95AGGAIAAGNASVSDD268 pKa = 3.49KK269 pKa = 11.15RR270 pKa = 11.84GMGDD274 pKa = 3.56YY275 pKa = 10.64YY276 pKa = 11.16VVDD279 pKa = 3.65MFICEE284 pKa = 4.31APQSSSSQLIVVPEE298 pKa = 3.81ATLYY302 pKa = 9.06WHH304 pKa = 7.08EE305 pKa = 4.26KK306 pKa = 9.3

Molecular weight: 34.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

842

204

332

280.7

31.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.838 ± 0.003

2.257 ± 0.502

7.126 ± 1.017

3.682 ± 0.655

4.513 ± 0.551

9.145 ± 0.142

2.613 ± 0.88

4.513 ± 0.421

4.869 ± 0.961

7.363 ± 1.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.781 ± 0.799

4.513 ± 1.19

4.632 ± 0.9

3.088 ± 0.381

6.888 ± 0.382

6.888 ± 0.473

5.819 ± 0.892

5.107 ± 0.077

2.732 ± 0.342

4.632 ± 0.851
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski