Yersinia phage vB_YenP_ISAO8
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H4TGZ7|A0A0H4TGZ7_9CAUD Uncharacterized protein OS=Yersinia phage vB_YenP_ISAO8 OX=1675027 PE=4 SV=1
MM1 pKa = 7.63 AKK3 pKa = 9.59 KK4 pKa = 10.05 QRR6 pKa = 11.84 YY7 pKa = 8.62 LNGTIFYY14 pKa = 9.94 RR15 pKa = 11.84 FDD17 pKa = 3.3 GTALRR22 pKa = 11.84 PWVQQVNGVQAGPFATLEE40 pKa = 4.16 DD41 pKa = 3.68 AQAALDD47 pKa = 4.23 SSNDD51 pKa = 3.27 PDD53 pKa = 3.9 NFDD56 pKa = 5.48 LIGSLEE62 pKa = 3.92 MRR64 pKa = 11.84 KK65 pKa = 9.85 VKK67 pKa = 10.7 LNEE70 pKa = 4.07 QPDD73 pKa = 3.91 GTTQCEE79 pKa = 4.54 SFCMAEE85 pKa = 3.86 EE86 pKa = 4.66 TPFDD90 pKa = 3.68 VYY92 pKa = 11.39 GLYY95 pKa = 11.01 GRR97 pKa = 11.84 TTDD100 pKa = 4.83 GYY102 pKa = 10.88 LLHH105 pKa = 6.89 LADD108 pKa = 4.15 YY109 pKa = 10.75 DD110 pKa = 3.56 RR111 pKa = 11.84 YY112 pKa = 10.27 AAVRR116 pKa = 11.84 NQYY119 pKa = 11.38 ALLIDD124 pKa = 3.83 RR125 pKa = 11.84 LGNGPVDD132 pKa = 3.51 YY133 pKa = 10.85 RR134 pKa = 11.84 EE135 pKa = 4.23 LLEE138 pKa = 4.61 AVQQ141 pKa = 4.25
Molecular weight: 15.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.385
IPC2_protein 4.482
IPC_protein 4.406
Toseland 4.215
ProMoST 4.533
Dawson 4.368
Bjellqvist 4.52
Wikipedia 4.279
Rodwell 4.228
Grimsley 4.126
Solomon 4.368
Lehninger 4.317
Nozaki 4.482
DTASelect 4.698
Thurlkill 4.24
EMBOSS 4.291
Sillero 4.52
Patrickios 3.401
IPC_peptide 4.368
IPC2_peptide 4.495
IPC2.peptide.svr19 4.479
Protein with the highest isoelectric point:
>tr|A0A0H4U2C1|A0A0H4U2C1_9CAUD Uncharacterized protein OS=Yersinia phage vB_YenP_ISAO8 OX=1675027 PE=4 SV=1
MM1 pKa = 7.48 TYY3 pKa = 10.85 KK4 pKa = 10.42 NRR6 pKa = 11.84 NASPEE11 pKa = 3.88 YY12 pKa = 9.93 RR13 pKa = 11.84 KK14 pKa = 10.47 AIAARR19 pKa = 11.84 TARR22 pKa = 11.84 RR23 pKa = 11.84 EE24 pKa = 3.63 AAARR28 pKa = 11.84 AKK30 pKa = 10.65 LEE32 pKa = 3.92 QDD34 pKa = 2.94 QMQWTTEE41 pKa = 3.74 GDD43 pKa = 3.73 FSHH46 pKa = 7.23 LAGNNTMKK54 pKa = 10.26 FRR56 pKa = 11.84 RR57 pKa = 11.84 GG58 pKa = 3.12
Molecular weight: 6.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.736
IPC_protein 10.599
Toseland 10.76
ProMoST 10.482
Dawson 10.847
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.052
Grimsley 10.906
Solomon 10.994
Lehninger 10.965
Nozaki 10.73
DTASelect 10.57
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 10.847
IPC_peptide 10.994
IPC2_peptide 9.355
IPC2.peptide.svr19 8.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
12760
46
930
277.4
30.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.82 ± 0.626
0.862 ± 0.133
6.168 ± 0.192
6.552 ± 0.348
3.597 ± 0.219
7.547 ± 0.287
1.975 ± 0.158
4.53 ± 0.238
6.23 ± 0.278
7.908 ± 0.32
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.346 ± 0.189
4.522 ± 0.252
4.013 ± 0.221
4.514 ± 0.4
5.752 ± 0.283
5.282 ± 0.297
5.666 ± 0.284
6.544 ± 0.279
1.74 ± 0.195
3.433 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here