Mycobacterium phage BigMama

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dclasvirinae; Plotvirus; Mycobacterium virus Plot

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1N588|A0A2P1N588_9CAUD Uncharacterized protein OS=Mycobacterium phage BigMama OX=2126786 GN=40 PE=4 SV=1
MM1 pKa = 7.72SEE3 pKa = 4.08LCALCGHH10 pKa = 6.41EE11 pKa = 4.76FEE13 pKa = 4.79EE14 pKa = 5.82HH15 pKa = 5.88YY16 pKa = 11.14SQGDD20 pKa = 3.66SNGRR24 pKa = 11.84LVQGCEE30 pKa = 3.97ADD32 pKa = 3.27TSTPCPAYY40 pKa = 10.15NHH42 pKa = 7.28RR43 pKa = 11.84DD44 pKa = 3.74PEE46 pKa = 4.25RR47 pKa = 11.84CEE49 pKa = 4.07CDD51 pKa = 4.35YY52 pKa = 11.42VPCDD56 pKa = 3.31CPGFEE61 pKa = 4.92RR62 pKa = 11.84EE63 pKa = 4.75PGTDD67 pKa = 3.47PDD69 pKa = 3.29ITEE72 pKa = 3.77IDD74 pKa = 3.48MYY76 pKa = 11.19DD77 pKa = 3.3PEE79 pKa = 4.42GRR81 pKa = 3.66

Molecular weight: 9.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1N5J1|A0A2P1N5J1_9CAUD Thymidylate synthase OS=Mycobacterium phage BigMama OX=2126786 GN=67 PE=4 SV=1
MM1 pKa = 7.23LRR3 pKa = 11.84YY4 pKa = 10.14YY5 pKa = 10.55FDD7 pKa = 4.05RR8 pKa = 11.84GLRR11 pKa = 11.84IFGVTVQNRR20 pKa = 11.84VDD22 pKa = 4.01DD23 pKa = 4.83AGHH26 pKa = 5.96AAGRR30 pKa = 11.84GAKK33 pKa = 9.81NNRR36 pKa = 11.84NRR38 pKa = 11.84EE39 pKa = 3.94ALQNGARR46 pKa = 11.84KK47 pKa = 8.34RR48 pKa = 11.84TLAKK52 pKa = 10.7LLGEE56 pKa = 4.54DD57 pKa = 4.05MVSSSAGFRR66 pKa = 11.84DD67 pKa = 3.44PAKK70 pKa = 10.63AVV72 pKa = 3.17

Molecular weight: 7.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

20526

34

1590

235.9

26.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.203 ± 0.334

1.004 ± 0.124

6.903 ± 0.378

6.879 ± 0.252

3.211 ± 0.121

8.297 ± 0.321

1.92 ± 0.153

4.882 ± 0.234

5.593 ± 0.268

7.595 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.09

3.888 ± 0.164

5.15 ± 0.186

3.595 ± 0.155

6.548 ± 0.353

5.988 ± 0.225

5.856 ± 0.246

6.182 ± 0.212

1.963 ± 0.124

3.094 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski