Clostridium tagluense
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4934 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A401UG75|A0A401UG75_9CLOT ATP synthase subunit alpha OS=Clostridium tagluense OX=360422 GN=atpA_2 PE=3 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.46 SVIKK6 pKa = 9.8 VCNMNTSDD14 pKa = 5.6 DD15 pKa = 3.48 ISNINVAISNNQGVIACQINSEE37 pKa = 4.29 SKK39 pKa = 10.28 EE40 pKa = 3.62 IEE42 pKa = 4.33 IIYY45 pKa = 10.5 DD46 pKa = 3.6 DD47 pKa = 4.48 YY48 pKa = 11.45 FVSLDD53 pKa = 3.55 NLIEE57 pKa = 4.58 SIEE60 pKa = 4.1 DD61 pKa = 2.78 AGYY64 pKa = 11.15 AVII67 pKa = 5.63
Molecular weight: 7.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.808
IPC_protein 3.681
Toseland 3.49
ProMoST 3.872
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.414
Solomon 3.643
Lehninger 3.605
Nozaki 3.834
DTASelect 3.999
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.808
Patrickios 0.604
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|A0A401UT22|A0A401UT22_9CLOT Uncharacterized protein OS=Clostridium tagluense OX=360422 GN=Ctaglu_42870 PE=4 SV=1
MM1 pKa = 7.57 GNLKK5 pKa = 10.46 NKK7 pKa = 9.65 LAQFMYY13 pKa = 10.44 GRR15 pKa = 11.84 YY16 pKa = 8.36 GTDD19 pKa = 2.7 QLYY22 pKa = 10.13 NALIVVYY29 pKa = 9.05 FVLIVANAFISSAIIRR45 pKa = 11.84 ILMSAVLIWTIFRR58 pKa = 11.84 ALSRR62 pKa = 11.84 NVYY65 pKa = 8.56 KK66 pKa = 10.62 RR67 pKa = 11.84 RR68 pKa = 11.84 MEE70 pKa = 3.87 NEE72 pKa = 3.53 KK73 pKa = 10.46 FMKK76 pKa = 9.26 IWKK79 pKa = 7.97 PVKK82 pKa = 10.44 GSGSLMLRR90 pKa = 11.84 RR91 pKa = 11.84 IKK93 pKa = 10.05 EE94 pKa = 4.03 IKK96 pKa = 6.27 THH98 pKa = 6.4 RR99 pKa = 11.84 FRR101 pKa = 11.84 KK102 pKa = 9.8 CPHH105 pKa = 6.02 CKK107 pKa = 9.6 KK108 pKa = 10.39 VLRR111 pKa = 11.84 LPRR114 pKa = 11.84 TRR116 pKa = 11.84 GKK118 pKa = 10.1 HH119 pKa = 4.52 IVKK122 pKa = 10.1 CPCCNKK128 pKa = 9.91 EE129 pKa = 4.07 FEE131 pKa = 4.51 LQILLL136 pKa = 4.21
Molecular weight: 16.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.853
IPC_protein 10.379
Toseland 10.818
ProMoST 10.409
Dawson 10.906
Bjellqvist 10.555
Wikipedia 11.067
Rodwell 11.33
Grimsley 10.935
Solomon 10.979
Lehninger 10.95
Nozaki 10.804
DTASelect 10.555
Thurlkill 10.804
EMBOSS 11.199
Sillero 10.833
Patrickios 11.052
IPC_peptide 10.994
IPC2_peptide 9.575
IPC2.peptide.svr19 8.482
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4934
0
4934
1433567
39
2876
290.5
32.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.7 ± 0.039
1.271 ± 0.016
5.379 ± 0.026
7.179 ± 0.038
4.411 ± 0.029
6.304 ± 0.032
1.403 ± 0.015
10.044 ± 0.039
9.141 ± 0.04
8.969 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.778 ± 0.018
6.215 ± 0.032
2.699 ± 0.019
2.544 ± 0.017
3.2 ± 0.023
6.424 ± 0.027
5.14 ± 0.03
6.426 ± 0.035
0.711 ± 0.011
4.061 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here