Tortoise microvirus 27

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4P8W647|A0A4P8W647_9VIRU Major capsid protein OS=Tortoise microvirus 27 OX=2583129 PE=3 SV=1
MM1 pKa = 7.67PFSGKK6 pKa = 9.92LVEE9 pKa = 4.76VKK11 pKa = 7.35TTKK14 pKa = 10.25PKK16 pKa = 10.2IYY18 pKa = 10.31SRR20 pKa = 11.84WNRR23 pKa = 11.84VRR25 pKa = 11.84EE26 pKa = 4.01EE27 pKa = 4.13VVEE30 pKa = 4.72DD31 pKa = 3.77EE32 pKa = 4.8TPSIVDD38 pKa = 3.1PAGYY42 pKa = 10.33VDD44 pKa = 3.59PNKK47 pKa = 10.55RR48 pKa = 11.84IEE50 pKa = 4.23EE51 pKa = 4.21YY52 pKa = 9.92IKK54 pKa = 10.63AGMRR58 pKa = 11.84IDD60 pKa = 2.87AWNRR64 pKa = 11.84SVFDD68 pKa = 4.05YY69 pKa = 11.2EE70 pKa = 4.18NEE72 pKa = 3.82QDD74 pKa = 3.8GEE76 pKa = 4.35EE77 pKa = 4.14KK78 pKa = 10.65FDD80 pKa = 3.42VEE82 pKa = 4.82EE83 pKa = 4.19YY84 pKa = 11.11DD85 pKa = 5.2GINDD89 pKa = 4.28DD90 pKa = 6.08LEE92 pKa = 4.33DD93 pKa = 3.73LRR95 pKa = 11.84KK96 pKa = 10.51GEE98 pKa = 4.05ISLGRR103 pKa = 11.84YY104 pKa = 5.32QQTMEE109 pKa = 4.27YY110 pKa = 9.82LYY112 pKa = 10.9KK113 pKa = 10.52SYY115 pKa = 11.08LSQQDD120 pKa = 3.66PQEE123 pKa = 4.29VTDD126 pKa = 4.21EE127 pKa = 4.25QQSSNSATDD136 pKa = 3.71NNQSATDD143 pKa = 3.7SKK145 pKa = 9.8EE146 pKa = 4.08TKK148 pKa = 10.13

Molecular weight:
17.25 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4V1FVX5|A0A4V1FVX5_9VIRU DNA pilot protein OS=Tortoise microvirus 27 OX=2583129 PE=4 SV=1
MM1 pKa = 7.08NSKK4 pKa = 9.69VRR6 pKa = 11.84TALRR10 pKa = 11.84IIIKK14 pKa = 9.95ALRR17 pKa = 11.84IAKK20 pKa = 9.82RR21 pKa = 11.84LSNSRR26 pKa = 11.84GIQKK30 pKa = 9.62CQKK33 pKa = 8.89EE34 pKa = 4.42LKK36 pKa = 10.42DD37 pKa = 4.06LIKK40 pKa = 10.54NHH42 pKa = 6.65PEE44 pKa = 3.29IDD46 pKa = 3.46VGDD49 pKa = 4.55FIIEE53 pKa = 4.28SGLTTQEE60 pKa = 4.01LGLEE64 pKa = 4.18KK65 pKa = 10.31LVKK68 pKa = 10.38RR69 pKa = 11.84STGVNPISS77 pKa = 3.49

Molecular weight:
8.63 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1438

77

547

239.7

27.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.981 ± 1.748

1.46 ± 0.511

5.981 ± 0.729

5.633 ± 1.338

3.06 ± 0.724

7.51 ± 1.051

1.599 ± 0.27

6.328 ± 0.568

5.285 ± 2.09

7.719 ± 0.707

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.851 ± 0.246

5.772 ± 0.494

5.146 ± 0.821

6.328 ± 1.29

6.606 ± 0.248

5.424 ± 0.545

5.911 ± 0.428

6.05 ± 0.479

1.252 ± 0.199

4.103 ± 0.939

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski