Tortoise microvirus 27
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W647|A0A4P8W647_9VIRU Major capsid protein OS=Tortoise microvirus 27 OX=2583129 PE=3 SV=1
MM1 pKa = 7.67 PFSGKK6 pKa = 9.92 LVEE9 pKa = 4.76 VKK11 pKa = 7.35 TTKK14 pKa = 10.25 PKK16 pKa = 10.2 IYY18 pKa = 10.31 SRR20 pKa = 11.84 WNRR23 pKa = 11.84 VRR25 pKa = 11.84 EE26 pKa = 4.01 EE27 pKa = 4.13 VVEE30 pKa = 4.72 DD31 pKa = 3.77 EE32 pKa = 4.8 TPSIVDD38 pKa = 3.1 PAGYY42 pKa = 10.33 VDD44 pKa = 3.59 PNKK47 pKa = 10.55 RR48 pKa = 11.84 IEE50 pKa = 4.23 EE51 pKa = 4.21 YY52 pKa = 9.92 IKK54 pKa = 10.63 AGMRR58 pKa = 11.84 IDD60 pKa = 2.87 AWNRR64 pKa = 11.84 SVFDD68 pKa = 4.05 YY69 pKa = 11.2 EE70 pKa = 4.18 NEE72 pKa = 3.82 QDD74 pKa = 3.8 GEE76 pKa = 4.35 EE77 pKa = 4.14 KK78 pKa = 10.65 FDD80 pKa = 3.42 VEE82 pKa = 4.82 EE83 pKa = 4.19 YY84 pKa = 11.11 DD85 pKa = 5.2 GINDD89 pKa = 4.28 DD90 pKa = 6.08 LEE92 pKa = 4.33 DD93 pKa = 3.73 LRR95 pKa = 11.84 KK96 pKa = 10.51 GEE98 pKa = 4.05 ISLGRR103 pKa = 11.84 YY104 pKa = 5.32 QQTMEE109 pKa = 4.27 YY110 pKa = 9.82 LYY112 pKa = 10.9 KK113 pKa = 10.52 SYY115 pKa = 11.08 LSQQDD120 pKa = 3.66 PQEE123 pKa = 4.29 VTDD126 pKa = 4.21 EE127 pKa = 4.25 QQSSNSATDD136 pKa = 3.71 NNQSATDD143 pKa = 3.7 SKK145 pKa = 9.8 EE146 pKa = 4.08 TKK148 pKa = 10.13
Molecular weight: 17.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.46
IPC2_protein 4.355
IPC_protein 4.291
Toseland 4.113
ProMoST 4.393
Dawson 4.24
Bjellqvist 4.393
Wikipedia 4.113
Rodwell 4.126
Grimsley 4.024
Solomon 4.228
Lehninger 4.19
Nozaki 4.342
DTASelect 4.507
Thurlkill 4.126
EMBOSS 4.126
Sillero 4.393
Patrickios 3.859
IPC_peptide 4.24
IPC2_peptide 4.38
IPC2.peptide.svr19 4.341
Protein with the highest isoelectric point:
>tr|A0A4V1FVX5|A0A4V1FVX5_9VIRU DNA pilot protein OS=Tortoise microvirus 27 OX=2583129 PE=4 SV=1
MM1 pKa = 7.08 NSKK4 pKa = 9.69 VRR6 pKa = 11.84 TALRR10 pKa = 11.84 IIIKK14 pKa = 9.95 ALRR17 pKa = 11.84 IAKK20 pKa = 9.82 RR21 pKa = 11.84 LSNSRR26 pKa = 11.84 GIQKK30 pKa = 9.62 CQKK33 pKa = 8.89 EE34 pKa = 4.42 LKK36 pKa = 10.42 DD37 pKa = 4.06 LIKK40 pKa = 10.54 NHH42 pKa = 6.65 PEE44 pKa = 3.29 IDD46 pKa = 3.46 VGDD49 pKa = 4.55 FIIEE53 pKa = 4.28 SGLTTQEE60 pKa = 4.01 LGLEE64 pKa = 4.18 KK65 pKa = 10.31 LVKK68 pKa = 10.38 RR69 pKa = 11.84 STGVNPISS77 pKa = 3.49
Molecular weight: 8.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.059
IPC2_protein 9.238
IPC_protein 9.238
Toseland 10.511
ProMoST 9.897
Dawson 10.555
Bjellqvist 10.101
Wikipedia 10.628
Rodwell 11.199
Grimsley 10.555
Solomon 10.599
Lehninger 10.599
Nozaki 10.482
DTASelect 10.087
Thurlkill 10.467
EMBOSS 10.877
Sillero 10.467
Patrickios 10.979
IPC_peptide 10.613
IPC2_peptide 8.492
IPC2.peptide.svr19 8.35
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1438
77
547
239.7
27.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.981 ± 1.748
1.46 ± 0.511
5.981 ± 0.729
5.633 ± 1.338
3.06 ± 0.724
7.51 ± 1.051
1.599 ± 0.27
6.328 ± 0.568
5.285 ± 2.09
7.719 ± 0.707
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.851 ± 0.246
5.772 ± 0.494
5.146 ± 0.821
6.328 ± 1.29
6.606 ± 0.248
5.424 ± 0.545
5.911 ± 0.428
6.05 ± 0.479
1.252 ± 0.199
4.103 ± 0.939
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here