Hymenobacter crusticola

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5735 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A243WC56|A0A243WC56_9BACT Aspartate kinase OS=Hymenobacter crusticola OX=1770526 GN=BXP70_15450 PE=4 SV=1
MM1 pKa = 7.78LSTLTYY7 pKa = 9.16TALPSQEE14 pKa = 4.14KK15 pKa = 10.15LQAICKK21 pKa = 9.65AVAVLDD27 pKa = 4.05ALNSPEE33 pKa = 3.95YY34 pKa = 9.84EE35 pKa = 3.94YY36 pKa = 10.73RR37 pKa = 11.84YY38 pKa = 10.45YY39 pKa = 11.01SYY41 pKa = 11.72NKK43 pKa = 8.7GWAEE47 pKa = 3.88GEE49 pKa = 4.53EE50 pKa = 4.26VFEE53 pKa = 4.28MQDD56 pKa = 3.46GEE58 pKa = 4.48GDD60 pKa = 3.23QMLILFRR67 pKa = 11.84AEE69 pKa = 3.71GCVINGYY76 pKa = 10.61ASDD79 pKa = 4.11YY80 pKa = 11.21EE81 pKa = 4.26EE82 pKa = 4.45SDD84 pKa = 3.4KK85 pKa = 11.72NQLTRR90 pKa = 11.84GLPDD94 pKa = 3.2VFDD97 pKa = 3.76EE98 pKa = 5.65FIFGEE103 pKa = 4.39PVNSIGTTFCLWYY116 pKa = 9.44TPAHH120 pKa = 6.54KK121 pKa = 9.62WQIGQLEE128 pKa = 4.18TDD130 pKa = 3.51EE131 pKa = 5.61DD132 pKa = 4.32GSEE135 pKa = 3.98NFLDD139 pKa = 3.82IFDD142 pKa = 5.19GNPHH146 pKa = 6.62TYY148 pKa = 9.9IEE150 pKa = 4.39WASEE154 pKa = 4.33YY155 pKa = 10.81YY156 pKa = 10.65DD157 pKa = 3.9EE158 pKa = 5.76DD159 pKa = 3.46QDD161 pKa = 4.56RR162 pKa = 11.84PAPSLDD168 pKa = 3.19AVTSIYY174 pKa = 9.92QGKK177 pKa = 8.58PLTKK181 pKa = 10.18DD182 pKa = 2.91LALAIVPHH190 pKa = 7.05VEE192 pKa = 3.64DD193 pKa = 3.49WQQLEE198 pKa = 4.57RR199 pKa = 11.84DD200 pKa = 3.94LQEE203 pKa = 4.03IGYY206 pKa = 8.75PYY208 pKa = 10.89DD209 pKa = 3.7FNN211 pKa = 4.94

Molecular weight: 24.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A243W8M4|A0A243W8M4_9BACT Uncharacterized protein OS=Hymenobacter crusticola OX=1770526 GN=BXP70_24500 PE=4 SV=1
MM1 pKa = 7.57LKK3 pKa = 10.57FLGGPVAGAAAGLPPSLIWGLTVAGMMTSVVVVSGLGRR41 pKa = 11.84AWALHH46 pKa = 4.11QRR48 pKa = 11.84QRR50 pKa = 11.84RR51 pKa = 11.84QLRR54 pKa = 11.84GKK56 pKa = 9.04PLFSKK61 pKa = 10.33RR62 pKa = 11.84SRR64 pKa = 11.84GIVRR68 pKa = 11.84IFRR71 pKa = 11.84RR72 pKa = 11.84FGMPGIAFLTPILFSPIGGTVIATQLHH99 pKa = 5.21VPRR102 pKa = 11.84TRR104 pKa = 11.84ILFHH108 pKa = 6.11MLWSAAFWAAALTMLACRR126 pKa = 11.84FSHH129 pKa = 7.16LSIFHH134 pKa = 6.56HH135 pKa = 6.84

Molecular weight: 14.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5735

0

5735

2016218

26

2915

351.6

38.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.886 ± 0.038

0.697 ± 0.009

4.943 ± 0.024

5.147 ± 0.036

4.136 ± 0.019

7.457 ± 0.035

2.046 ± 0.017

4.548 ± 0.027

4.279 ± 0.035

10.748 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.821 ± 0.015

4.167 ± 0.033

4.996 ± 0.022

4.905 ± 0.026

5.613 ± 0.027

5.852 ± 0.027

6.58 ± 0.04

7.169 ± 0.026

1.296 ± 0.014

3.713 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski