Lactococcus phage 62502

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vedamuthuvirus; unclassified Vedamuthuvirus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8BL98|A0A1P8BL98_9CAUD Head-tail adapter protein OS=Lactococcus phage 62502 OX=1868852 GN=DS62502_40 PE=4 SV=1
MM1 pKa = 8.08RR2 pKa = 11.84KK3 pKa = 7.78MTDD6 pKa = 2.99KK7 pKa = 11.14LISLVNDD14 pKa = 3.12WRR16 pKa = 11.84GGFYY20 pKa = 10.77LMPEE24 pKa = 4.1DD25 pKa = 4.04TPEE28 pKa = 3.89EE29 pKa = 3.99EE30 pKa = 4.03LRR32 pKa = 11.84EE33 pKa = 4.04VEE35 pKa = 5.18VYY37 pKa = 10.96CDD39 pKa = 3.23TCGDD43 pKa = 3.54NDD45 pKa = 4.7SIIGQFSNWNQLKK58 pKa = 10.45KK59 pKa = 11.08EE60 pKa = 4.06MTDD63 pKa = 3.5DD64 pKa = 4.56EE65 pKa = 5.2GWCPYY70 pKa = 10.28SDD72 pKa = 5.14EE73 pKa = 4.2YY74 pKa = 10.46LQSVFEE80 pKa = 4.85EE81 pKa = 4.86DD82 pKa = 3.67DD83 pKa = 3.23QQ84 pKa = 6.2

Molecular weight: 9.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8BLB4|A0A1P8BLB4_9CAUD Distal tail protein OS=Lactococcus phage 62502 OX=1868852 GN=DS62502_47 PE=4 SV=1
MM1 pKa = 7.72LFQIQIQITKK11 pKa = 10.36CFGRR15 pKa = 11.84DD16 pKa = 2.96KK17 pKa = 11.44APFSKK22 pKa = 10.51ARR24 pKa = 11.84ALFLCYY30 pKa = 10.22NIVGMFVRR38 pKa = 11.84FHH40 pKa = 7.12PIPRR44 pKa = 11.84VKK46 pKa = 10.41PFFGVAFFVCCKK58 pKa = 8.66FTTILSEE65 pKa = 4.18TT66 pKa = 3.81

Molecular weight: 7.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

9602

44

733

184.7

20.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.54 ± 0.531

0.562 ± 0.103

6.332 ± 0.348

7.103 ± 0.498

4.603 ± 0.302

6.686 ± 0.468

1.281 ± 0.175

7.186 ± 0.367

9.134 ± 0.421

7.738 ± 0.376

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.458 ± 0.153

6.27 ± 0.339

2.666 ± 0.18

4.624 ± 0.44

3.749 ± 0.25

5.936 ± 0.271

6.113 ± 0.34

5.863 ± 0.299

1.25 ± 0.138

3.905 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski