Fusarium poae virus 1-240374
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4ZA50|A0A1B4ZA50_9VIRU RNA-dependent RNA polymerase OS=Fusarium poae virus 1-240374 OX=1849534 PE=4 SV=1
MM1 pKa = 7.2 SQQNNARR8 pKa = 11.84 VSTASRR14 pKa = 11.84 TSSVEE19 pKa = 3.41 LRR21 pKa = 11.84 TPAAIAAIAKK31 pKa = 9.59 RR32 pKa = 11.84 FAPVVFQTDD41 pKa = 3.83 DD42 pKa = 3.77 TPVPPSNADD51 pKa = 2.94 YY52 pKa = 9.79 FARR55 pKa = 11.84 MNMPSNIEE63 pKa = 4.18 LDD65 pKa = 3.6 SGDD68 pKa = 3.91 YY69 pKa = 9.97 ATIDD73 pKa = 3.42 VQFDD77 pKa = 3.17 TRR79 pKa = 11.84 FIMNYY84 pKa = 9.55 ILYY87 pKa = 9.28 HH88 pKa = 5.16 ATNRR92 pKa = 11.84 APEE95 pKa = 4.2 MNFKK99 pKa = 9.78 GHH101 pKa = 7.34 PYY103 pKa = 8.65 ATPLSYY109 pKa = 10.79 AGYY112 pKa = 10.11 CLTLLYY118 pKa = 10.69 TFMLAVDD125 pKa = 3.69 VTTRR129 pKa = 11.84 PEE131 pKa = 4.3 KK132 pKa = 9.75 SWHH135 pKa = 5.87 AARR138 pKa = 11.84 FMSDD142 pKa = 3.07 AEE144 pKa = 4.27 RR145 pKa = 11.84 KK146 pKa = 9.65 DD147 pKa = 3.75 LYY149 pKa = 11.15 DD150 pKa = 3.22 VLLSSRR156 pKa = 11.84 VPTWFVDD163 pKa = 3.67 LLIEE167 pKa = 4.18 FAPVFDD173 pKa = 4.12 PRR175 pKa = 11.84 RR176 pKa = 11.84 NNILMVPTLAGYY188 pKa = 9.0 IHH190 pKa = 6.96 NIDD193 pKa = 4.0 FGRR196 pKa = 11.84 TFPPSIFYY204 pKa = 10.4 SAHH207 pKa = 6.03 NLLASTRR214 pKa = 11.84 TNLDD218 pKa = 3.26 PDD220 pKa = 3.83 NVIDD224 pKa = 4.54 SLMGTPLINHH234 pKa = 6.41 GTNAYY239 pKa = 7.58 TVSNYY244 pKa = 10.67 LGTWYY249 pKa = 10.11 DD250 pKa = 3.63 AGHH253 pKa = 6.8 HH254 pKa = 6.4 PNFVNQDD261 pKa = 3.28 YY262 pKa = 9.77 MSFFNPLVGRR272 pKa = 11.84 FLTQRR277 pKa = 11.84 PTFARR282 pKa = 11.84 LPFSPEE288 pKa = 3.59 DD289 pKa = 3.54 LADD292 pKa = 3.71 NGIGNIYY299 pKa = 7.68 TTFLLASDD307 pKa = 4.47 EE308 pKa = 4.65 NISLANTLYY317 pKa = 10.13 TAMSTFILSDD327 pKa = 3.67 EE328 pKa = 4.58 PNAPQLGSVLSSLSGSLLLSHH349 pKa = 7.09 SIEE352 pKa = 5.29 PITLPTWTKK361 pKa = 8.58 ATYY364 pKa = 10.64 VSDD367 pKa = 3.59 IDD369 pKa = 3.88 PSLVSDD375 pKa = 3.73 SKK377 pKa = 11.14 FVSDD381 pKa = 4.17 HH382 pKa = 6.2 NLFSRR387 pKa = 11.84 TPKK390 pKa = 10.25 YY391 pKa = 10.38 EE392 pKa = 3.88 KK393 pKa = 9.85 STLFPNDD400 pKa = 2.99 GKK402 pKa = 9.5 TITSLYY408 pKa = 10.5 YY409 pKa = 10.53 LVTKK413 pKa = 10.27 KK414 pKa = 10.56 KK415 pKa = 10.02 HH416 pKa = 6.15 DD417 pKa = 4.31 ANDD420 pKa = 3.43 NPTSHH425 pKa = 7.23 LSFKK429 pKa = 10.9 GKK431 pKa = 10.38 DD432 pKa = 3.24 SVTPYY437 pKa = 10.98 VLYY440 pKa = 9.31 FQPYY444 pKa = 8.43 DD445 pKa = 3.62 VSPSSLGLTIAAGLKK460 pKa = 9.86 IEE462 pKa = 4.23 LAEE465 pKa = 3.93 ISGFTVPVEE474 pKa = 4.18 HH475 pKa = 7.41 PEE477 pKa = 4.87 SSLLDD482 pKa = 3.63 NNSQYY487 pKa = 10.91 LQSAIRR493 pKa = 11.84 ANLIAPVNDD502 pKa = 3.24 RR503 pKa = 11.84 STAAEE508 pKa = 3.84 NSSLNLARR516 pKa = 11.84 SGLDD520 pKa = 2.98 STLQGITASFISTCKK535 pKa = 10.26 SVLPFFDD542 pKa = 4.57 NEE544 pKa = 4.22 GNNLGDD550 pKa = 3.73 STDD553 pKa = 3.37 LSKK556 pKa = 11.2 FGLTPEE562 pKa = 4.28 LHH564 pKa = 6.51 HH565 pKa = 6.43 VSPFAGFNVKK575 pKa = 10.33 AGTNGQLLSTEE586 pKa = 4.18 KK587 pKa = 10.64 SIHH590 pKa = 5.69 LWSSYY595 pKa = 10.17 RR596 pKa = 11.84 VVHH599 pKa = 5.64 TKK601 pKa = 10.43 KK602 pKa = 10.58 NPAARR607 pKa = 11.84 DD608 pKa = 3.29 ISFIASFRR616 pKa = 11.84 PIYY619 pKa = 8.34 GTNVTLSRR627 pKa = 11.84 SKK629 pKa = 10.85 NPSLIIPHH637 pKa = 6.89
Molecular weight: 70.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.705
IPC2_protein 5.614
IPC_protein 5.69
Toseland 6.224
ProMoST 5.995
Dawson 5.97
Bjellqvist 5.97
Wikipedia 5.995
Rodwell 5.957
Grimsley 6.453
Solomon 5.97
Lehninger 5.97
Nozaki 6.249
DTASelect 6.453
Thurlkill 6.453
EMBOSS 6.415
Sillero 6.338
Patrickios 3.91
IPC_peptide 5.982
IPC2_peptide 6.351
IPC2.peptide.svr19 6.263
Protein with the highest isoelectric point:
>tr|A0A1B4ZA50|A0A1B4ZA50_9VIRU RNA-dependent RNA polymerase OS=Fusarium poae virus 1-240374 OX=1849534 PE=4 SV=1
MM1 pKa = 7.52 LANIRR6 pKa = 11.84 DD7 pKa = 3.95 YY8 pKa = 11.13 FHH10 pKa = 7.4 EE11 pKa = 4.44 KK12 pKa = 9.0 LTRR15 pKa = 11.84 LLYY18 pKa = 9.99 DD19 pKa = 3.26 HH20 pKa = 7.49 KK21 pKa = 10.78 IFQSNSKK28 pKa = 11.0 DD29 pKa = 3.28 PDD31 pKa = 3.39 LTLEE35 pKa = 4.13 AHH37 pKa = 6.56 HH38 pKa = 6.79 SSDD41 pKa = 3.21 IEE43 pKa = 4.12 RR44 pKa = 11.84 IYY46 pKa = 11.22 KK47 pKa = 9.85 SIHH50 pKa = 5.92 YY51 pKa = 9.6 DD52 pKa = 3.59 FNRR55 pKa = 11.84 SPAPTDD61 pKa = 3.39 YY62 pKa = 10.42 EE63 pKa = 3.96 AQYY66 pKa = 11.49 QSIKK70 pKa = 10.51 HH71 pKa = 5.63 ILEE74 pKa = 4.86 DD75 pKa = 3.63 KK76 pKa = 10.58 QSQQGFPHH84 pKa = 7.08 EE85 pKa = 4.43 YY86 pKa = 9.97 YY87 pKa = 10.25 RR88 pKa = 11.84 LHH90 pKa = 7.04 EE91 pKa = 4.51 SPIPDD96 pKa = 3.99 DD97 pKa = 5.1 RR98 pKa = 11.84 IPPSGIKK105 pKa = 10.19 LLPFEE110 pKa = 4.54 YY111 pKa = 10.56 KK112 pKa = 10.65 SMNVVTATPEE122 pKa = 4.01 VPEE125 pKa = 4.58 SGFKK129 pKa = 9.18 IHH131 pKa = 6.9 PRR133 pKa = 11.84 IEE135 pKa = 3.62 RR136 pKa = 11.84 LLRR139 pKa = 11.84 SKK141 pKa = 10.78 YY142 pKa = 9.3 PQYY145 pKa = 10.38 LQYY148 pKa = 10.14 VRR150 pKa = 11.84 KK151 pKa = 7.66 YY152 pKa = 8.1 TRR154 pKa = 11.84 PLGTTNATVSDD165 pKa = 4.28 FFKK168 pKa = 10.55 PQTPSQPVEE177 pKa = 3.79 PTRR180 pKa = 11.84 INHH183 pKa = 5.16 VMSHH187 pKa = 5.22 VMKK190 pKa = 10.8 KK191 pKa = 8.65 MAITPYY197 pKa = 10.55 LPLHH201 pKa = 6.2 FVDD204 pKa = 3.53 TQYY207 pKa = 11.79 DD208 pKa = 3.77 KK209 pKa = 11.49 RR210 pKa = 11.84 PLANGTGYY218 pKa = 10.71 HH219 pKa = 5.67 NRR221 pKa = 11.84 RR222 pKa = 11.84 SHH224 pKa = 5.82 EE225 pKa = 4.14 MNIHH229 pKa = 6.49 ALFSHH234 pKa = 6.39 PKK236 pKa = 8.32 EE237 pKa = 4.24 YY238 pKa = 10.4 EE239 pKa = 3.87 SKK241 pKa = 8.84 RR242 pKa = 11.84 TSKK245 pKa = 10.71 GYY247 pKa = 9.76 YY248 pKa = 9.12 VNAFLEE254 pKa = 4.58 SARR257 pKa = 11.84 SLIHH261 pKa = 6.68 WIKK264 pKa = 10.88 LYY266 pKa = 10.97 GNPFRR271 pKa = 11.84 HH272 pKa = 6.43 CPSDD276 pKa = 3.31 LAQSLRR282 pKa = 11.84 EE283 pKa = 4.03 FFLQRR288 pKa = 11.84 PTMLFTRR295 pKa = 11.84 NHH297 pKa = 5.57 ISDD300 pKa = 3.52 RR301 pKa = 11.84 DD302 pKa = 4.03 GILKK306 pKa = 9.32 QRR308 pKa = 11.84 PVYY311 pKa = 10.63 AVDD314 pKa = 5.09 DD315 pKa = 4.18 LFLTIEE321 pKa = 4.17 SMLTFPAHH329 pKa = 5.69 VIARR333 pKa = 11.84 KK334 pKa = 9.29 PEE336 pKa = 3.9 CCIMYY341 pKa = 10.3 GLEE344 pKa = 4.27 TIRR347 pKa = 11.84 GSNQILDD354 pKa = 4.81 KK355 pKa = 10.76 IASDD359 pKa = 3.62 YY360 pKa = 11.53 KK361 pKa = 11.26 SFFTIDD367 pKa = 2.65 WSGFDD372 pKa = 4.51 QRR374 pKa = 11.84 LPWVIVKK381 pKa = 9.66 LFFTEE386 pKa = 4.23 YY387 pKa = 9.97 IPRR390 pKa = 11.84 LLVVNHH396 pKa = 6.92 GYY398 pKa = 11.03 APTYY402 pKa = 9.02 EE403 pKa = 4.25 YY404 pKa = 10.34 PSYY407 pKa = 10.98 PDD409 pKa = 3.35 LTTNDD414 pKa = 3.23 MVSRR418 pKa = 11.84 LTNLLTFLATWYY430 pKa = 10.46 FNMVFVTADD439 pKa = 2.87 GFSYY443 pKa = 10.54 VRR445 pKa = 11.84 EE446 pKa = 4.19 HH447 pKa = 6.82 AGVPSGMLNTQFLDD461 pKa = 3.44 SFGNLFLLIDD471 pKa = 3.82 GLIEE475 pKa = 4.54 FGSTDD480 pKa = 4.17 AEE482 pKa = 4.06 IDD484 pKa = 4.38 DD485 pKa = 3.94 ILLFIMGDD493 pKa = 3.62 DD494 pKa = 3.56 NSAFTTWSITHH505 pKa = 7.0 LEE507 pKa = 4.04 QFVSFFEE514 pKa = 4.66 TYY516 pKa = 10.23 ALSRR520 pKa = 11.84 YY521 pKa = 9.99 GMVLSKK527 pKa = 9.46 TKK529 pKa = 10.69 SIITTLRR536 pKa = 11.84 HH537 pKa = 5.96 KK538 pKa = 10.54 IEE540 pKa = 4.17 TLSYY544 pKa = 9.4 QCNFGHH550 pKa = 7.22 PRR552 pKa = 11.84 RR553 pKa = 11.84 PIGKK557 pKa = 9.07 LVAQLCFPEE566 pKa = 4.83 RR567 pKa = 11.84 GPRR570 pKa = 11.84 PKK572 pKa = 10.75 YY573 pKa = 8.73 MSARR577 pKa = 11.84 AVGMAWASCGQDD589 pKa = 2.84 KK590 pKa = 9.23 TFHH593 pKa = 6.55 DD594 pKa = 4.92 FCRR597 pKa = 11.84 DD598 pKa = 3.13 VYY600 pKa = 11.3 HH601 pKa = 6.92 EE602 pKa = 4.75 FNDD605 pKa = 4.1 DD606 pKa = 3.93 RR607 pKa = 11.84 ADD609 pKa = 4.03 LDD611 pKa = 3.62 EE612 pKa = 4.4 SAYY615 pKa = 11.34 LHH617 pKa = 6.24 IQSHH621 pKa = 6.05 LPGYY625 pKa = 10.41 LKK627 pKa = 9.66 IDD629 pKa = 3.35 EE630 pKa = 4.52 SVRR633 pKa = 11.84 QIVDD637 pKa = 3.74 FQVFPSQQTVYY648 pKa = 9.09 HH649 pKa = 5.79 TVSRR653 pKa = 11.84 WKK655 pKa = 10.9 GPLSYY660 pKa = 10.33 QPKK663 pKa = 8.42 WDD665 pKa = 3.55 LAHH668 pKa = 6.9 FVNQPDD674 pKa = 3.99 VVPPDD679 pKa = 3.4 SVTLYY684 pKa = 10.05 EE685 pKa = 4.5 VWSEE689 pKa = 4.44 LNFTPPILHH698 pKa = 6.6 NLFF701 pKa = 4.17
Molecular weight: 81.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.297
IPC2_protein 6.249
IPC_protein 6.376
Toseland 6.795
ProMoST 6.766
Dawson 6.722
Bjellqvist 6.707
Wikipedia 6.751
Rodwell 6.722
Grimsley 6.985
Solomon 6.737
Lehninger 6.737
Nozaki 7.0
DTASelect 7.19
Thurlkill 7.22
EMBOSS 7.19
Sillero 7.117
Patrickios 3.91
IPC_peptide 6.751
IPC2_peptide 6.971
IPC2.peptide.svr19 6.913
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1338
637
701
669.0
76.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.904 ± 1.101
0.673 ± 0.242
6.203 ± 0.055
3.961 ± 0.661
6.054 ± 0.272
4.185 ± 0.142
3.737 ± 0.615
5.68 ± 0.231
4.111 ± 0.444
9.791 ± 0.385
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.018 ± 0.197
5.082 ± 1.126
6.876 ± 0.021
2.99 ± 0.747
4.933 ± 0.575
9.193 ± 1.213
7.25 ± 0.723
5.306 ± 0.021
1.046 ± 0.177
5.007 ± 0.519
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here