Arcticibacterium luteifluviistationis
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4329 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4GB05|A0A2Z4GB05_9BACT Dehydrogenase OS=Arcticibacterium luteifluviistationis OX=1784714 GN=DJ013_09470 PE=4 SV=1
MM1 pKa = 7.12 STLKK5 pKa = 10.19 EE6 pKa = 4.1 KK7 pKa = 11.06 NMSNTLNKK15 pKa = 10.29 LLLLAFTLFAFIHH28 pKa = 6.12 EE29 pKa = 5.3 GYY31 pKa = 9.25 SQNNADD37 pKa = 4.12 PGIGILMSPSIVIQGSTGILSATVGNYY64 pKa = 9.93 GNEE67 pKa = 4.21 TIVEE71 pKa = 3.92 NSLRR75 pKa = 11.84 VTITVGSNAEE85 pKa = 4.23 IIGIAPGSDD94 pKa = 3.51 SRR96 pKa = 11.84 WSQLSLTTGSANSIKK111 pKa = 9.91 LTNTVGGFNSFDD123 pKa = 3.37 VGDD126 pKa = 3.6 ILLTVRR132 pKa = 11.84 GNLVSAPEE140 pKa = 4.73 LILGNIVYY148 pKa = 8.49 ITAEE152 pKa = 4.15 NPLLCDD158 pKa = 4.19 GCPSPPYY165 pKa = 10.26 NVSQGNASSLNDD177 pKa = 3.37 NSQTSLATSAPVIDD191 pKa = 4.29 AVVDD195 pKa = 3.65 ATAAVSGLTGGITSALTSNDD215 pKa = 3.39 SLNGIVVVIGIDD227 pKa = 3.42 SGNVTLTGLNVPAGLTLNADD247 pKa = 3.5 GTVTVDD253 pKa = 3.18 SNTPAGNYY261 pKa = 7.98 NVEE264 pKa = 4.13 YY265 pKa = 10.38 KK266 pKa = 10.11 ICEE269 pKa = 4.15 VNNPTNCDD277 pKa = 3.32 SVISMIVVSAPIIAAVVDD295 pKa = 3.84 TTAAVSGLTGGITSALTSNDD315 pKa = 3.39 SLNGIVVVIGIDD327 pKa = 3.24 SGNVMLTGLTVPAGLTLNADD347 pKa = 3.5 GTVTVDD353 pKa = 3.18 SNTPAGNYY361 pKa = 7.98 NVEE364 pKa = 4.13 YY365 pKa = 10.38 KK366 pKa = 10.11 ICEE369 pKa = 4.15 VNNPTNCDD377 pKa = 3.32 SVISMIVVSAPIIAAVVDD395 pKa = 3.84 TTAAVSGLTGGITSALTSNDD415 pKa = 3.39 SLNGIVVVIGIDD427 pKa = 3.42 SGNVTLTGLNVPAGLTLNADD447 pKa = 3.5 GTVTVDD453 pKa = 3.18 SNTPAGNYY461 pKa = 7.98 NVEE464 pKa = 4.13 YY465 pKa = 10.38 KK466 pKa = 10.11 ICEE469 pKa = 4.15 VNNPTNCDD477 pKa = 3.32 SVISMIVVSAPIIAAVVDD495 pKa = 3.84 TTAAVSGLTGGITSALTSNDD515 pKa = 3.39 SLNGIVVVIGIDD527 pKa = 3.24 SGNVMLTGLTVPAGLTLNADD547 pKa = 3.5 GTVTVDD553 pKa = 3.18 SNTPAGNYY561 pKa = 7.98 NVEE564 pKa = 4.13 YY565 pKa = 10.38 KK566 pKa = 10.11 ICEE569 pKa = 4.15 VNNPTNCDD577 pKa = 3.32 SVISMIVVSAPIIAAVVDD595 pKa = 3.84 TTAAVSGLTGGITSALTSNDD615 pKa = 3.39 SLNGIVVVIGIDD627 pKa = 3.42 SGNVTLTGLNVPAGLTLNADD647 pKa = 3.5 GTVTVDD653 pKa = 3.18 SNTPAGNYY661 pKa = 7.98 NVEE664 pKa = 4.13 YY665 pKa = 10.38 KK666 pKa = 10.11 ICEE669 pKa = 4.15 VNNPTNCDD677 pKa = 3.3 SVISMIEE684 pKa = 4.01 VYY686 pKa = 10.36 RR687 pKa = 11.84 EE688 pKa = 4.03 LPDD691 pKa = 3.88 LSPTIDD697 pKa = 2.97 IDD699 pKa = 4.03 ALVFPIAGSAKK710 pKa = 10.29 DD711 pKa = 3.52 FVVNIGDD718 pKa = 3.8 VKK720 pKa = 10.9 GVEE723 pKa = 4.04 SDD725 pKa = 3.52 GQVVVKK731 pKa = 10.09 VSKK734 pKa = 10.98 GNAFFISYY742 pKa = 10.84 ADD744 pKa = 3.45 STSNSNANGGSVPVNNSAWIITEE767 pKa = 3.72 NASFITITLKK777 pKa = 10.79 PGVQISANTFSAIGFTITRR796 pKa = 11.84 KK797 pKa = 10.31 AGVPTQTSQPITVTIVNGTGLDD819 pKa = 3.75 SQNSNNAYY827 pKa = 8.58 STIVTAQQ834 pKa = 2.84
Molecular weight: 84.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.643
IPC_protein 3.694
Toseland 3.452
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.503
Grimsley 3.363
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 4.075
Thurlkill 3.503
EMBOSS 3.643
Sillero 3.808
Patrickios 0.439
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.734
Protein with the highest isoelectric point:
>tr|A0A2Z4GBV0|A0A2Z4GBV0_9BACT Glycosyltransferase family 2 protein OS=Arcticibacterium luteifluviistationis OX=1784714 GN=DJ013_10660 PE=4 SV=1
MM1 pKa = 7.47 AVKK4 pKa = 10.11 KK5 pKa = 10.09 LKK7 pKa = 9.13 PTTPGQRR14 pKa = 11.84 QRR16 pKa = 11.84 VAPDD20 pKa = 3.76 FSDD23 pKa = 2.84 ITTNKK28 pKa = 9.57 PEE30 pKa = 3.92 RR31 pKa = 11.84 SLIGVVKK38 pKa = 9.62 KK39 pKa = 9.32 TGGRR43 pKa = 11.84 NNEE46 pKa = 3.75 GHH48 pKa = 5.56 RR49 pKa = 11.84 TSRR52 pKa = 11.84 YY53 pKa = 8.83 IGGGHH58 pKa = 5.9 KK59 pKa = 9.68 RR60 pKa = 11.84 RR61 pKa = 11.84 YY62 pKa = 9.75 RR63 pKa = 11.84 IIDD66 pKa = 3.74 FKK68 pKa = 10.85 RR69 pKa = 11.84 DD70 pKa = 3.24 KK71 pKa = 10.69 RR72 pKa = 11.84 DD73 pKa = 3.21 MVAEE77 pKa = 4.23 VATIEE82 pKa = 3.99 YY83 pKa = 10.37 DD84 pKa = 3.44 PNRR87 pKa = 11.84 SARR90 pKa = 11.84 IALVKK95 pKa = 11.01 YY96 pKa = 9.7 EE97 pKa = 4.73 DD98 pKa = 3.68 GEE100 pKa = 4.03 KK101 pKa = 10.23 RR102 pKa = 11.84 YY103 pKa = 10.2 IIAPQGLQVGQKK115 pKa = 9.38 IEE117 pKa = 4.6 AGDD120 pKa = 3.89 SVAPEE125 pKa = 3.88 VGNCLRR131 pKa = 11.84 VGNMPLGTIIHH142 pKa = 6.6 NIEE145 pKa = 3.96 LTPGKK150 pKa = 10.16 GGQLSRR156 pKa = 11.84 SAGTYY161 pKa = 9.86 AQLLAKK167 pKa = 10.1 DD168 pKa = 3.99 GKK170 pKa = 10.57 YY171 pKa = 8.43 VTLKK175 pKa = 9.96 MPSGEE180 pKa = 3.99 MRR182 pKa = 11.84 LVLSTCVATVGSVSNPDD199 pKa = 3.27 HH200 pKa = 6.53 MNVNLGKK207 pKa = 10.39 AGRR210 pKa = 11.84 NRR212 pKa = 11.84 WLGRR216 pKa = 11.84 RR217 pKa = 11.84 PRR219 pKa = 11.84 VRR221 pKa = 11.84 GVAMNPVDD229 pKa = 3.68 HH230 pKa = 7.13 PMGGGEE236 pKa = 3.89 GRR238 pKa = 11.84 ASGGHH243 pKa = 5.11 PRR245 pKa = 11.84 SRR247 pKa = 11.84 NGVMAKK253 pKa = 10.11 GKK255 pKa = 8.35 KK256 pKa = 7.69 TRR258 pKa = 11.84 SKK260 pKa = 10.76 NKK262 pKa = 9.01 YY263 pKa = 7.5 SNRR266 pKa = 11.84 LIISKK271 pKa = 10.21 RR272 pKa = 11.84 SKK274 pKa = 10.67
Molecular weight: 30.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.853
IPC_protein 10.628
Toseland 10.935
ProMoST 10.628
Dawson 11.008
Bjellqvist 10.701
Wikipedia 11.213
Rodwell 11.301
Grimsley 11.038
Solomon 11.14
Lehninger 11.111
Nozaki 10.906
DTASelect 10.701
Thurlkill 10.921
EMBOSS 11.33
Sillero 10.935
Patrickios 11.008
IPC_peptide 11.14
IPC2_peptide 9.531
IPC2.peptide.svr19 8.565
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4329
0
4329
1600995
38
7596
369.8
41.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.742 ± 0.04
0.808 ± 0.018
5.545 ± 0.031
6.59 ± 0.043
5.146 ± 0.039
7.099 ± 0.041
1.651 ± 0.017
7.31 ± 0.035
7.227 ± 0.055
9.372 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.218 ± 0.021
5.934 ± 0.047
3.594 ± 0.022
3.15 ± 0.023
3.391 ± 0.03
7.193 ± 0.056
5.756 ± 0.082
6.188 ± 0.029
1.178 ± 0.015
3.909 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here