Tea plant necrotic ring blotch virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Kitaviridae; Blunervirus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386QUK4|A0A386QUK4_9VIRU p24 OS=Tea plant necrotic ring blotch virus OX=2419939 PE=4 SV=1
MM1 pKa = 7.57FRR3 pKa = 11.84FRR5 pKa = 11.84DD6 pKa = 3.6LLTCVVVFIGSVEE19 pKa = 4.58EE20 pKa = 4.18IPQSLEE26 pKa = 3.87HH27 pKa = 6.33ICPYY31 pKa = 9.89EE32 pKa = 3.62IRR34 pKa = 11.84YY35 pKa = 9.9DD36 pKa = 3.98LVTHH40 pKa = 6.67NYY42 pKa = 9.72VSSNVYY48 pKa = 9.42KK49 pKa = 10.71LRR51 pKa = 11.84PKK53 pKa = 10.11VHH55 pKa = 6.63DD56 pKa = 3.94VYY58 pKa = 11.3RR59 pKa = 11.84KK60 pKa = 9.97LNSLTPVCRR69 pKa = 11.84RR70 pKa = 11.84AGEE73 pKa = 4.08KK74 pKa = 10.66LIDD77 pKa = 5.18DD78 pKa = 4.17ISYY81 pKa = 9.26TPTNFEE87 pKa = 4.39SLCVNNDD94 pKa = 3.13RR95 pKa = 11.84NPRR98 pKa = 11.84DD99 pKa = 3.36FNDD102 pKa = 3.36RR103 pKa = 11.84VIVFDD108 pKa = 4.57DD109 pKa = 3.51SCGQIHH115 pKa = 7.05RR116 pKa = 11.84SVNVPYY122 pKa = 10.54EE123 pKa = 3.69VGTYY127 pKa = 10.15GNCSWVCSTQVSSEE141 pKa = 3.72RR142 pKa = 11.84LTIVNGSCDD151 pKa = 3.88LSNHH155 pKa = 5.63PLRR158 pKa = 11.84VIDD161 pKa = 3.93HH162 pKa = 6.43SGVGYY167 pKa = 8.95VLSRR171 pKa = 11.84ITHH174 pKa = 6.43TILGLVEE181 pKa = 5.39DD182 pKa = 5.3FVSTVILTGFRR193 pKa = 11.84VLFEE197 pKa = 4.83LVFSLISTCYY207 pKa = 10.42DD208 pKa = 2.97VLEE211 pKa = 4.22PLKK214 pKa = 10.19PFEE217 pKa = 4.18VVMVSLLLFPYY228 pKa = 10.21VDD230 pKa = 2.81SCYY233 pKa = 10.43RR234 pKa = 11.84ILVGLFVFITMMVLPFF250 pKa = 4.39

Molecular weight: 28.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386QVR9|A0A386QVR9_9VIRU p22 OS=Tea plant necrotic ring blotch virus OX=2419939 PE=4 SV=1
MM1 pKa = 7.35FKK3 pKa = 10.78SIDD6 pKa = 3.88DD7 pKa = 5.12EE8 pKa = 4.18IDD10 pKa = 3.16KK11 pKa = 10.82LKK13 pKa = 10.68KK14 pKa = 9.66GRR16 pKa = 11.84KK17 pKa = 7.5EE18 pKa = 3.6KK19 pKa = 11.05EE20 pKa = 4.03KK21 pKa = 10.06ILKK24 pKa = 10.53ANFHH28 pKa = 5.69EE29 pKa = 4.77HH30 pKa = 5.51VKK32 pKa = 10.69SSGSIGEE39 pKa = 4.61LSPEE43 pKa = 4.67AIGSLNSKK51 pKa = 9.94YY52 pKa = 9.91EE53 pKa = 3.76AYY55 pKa = 10.3RR56 pKa = 11.84DD57 pKa = 3.79LKK59 pKa = 10.67SAYY62 pKa = 10.31ALDD65 pKa = 3.58TKK67 pKa = 11.01KK68 pKa = 10.82RR69 pKa = 11.84IDD71 pKa = 3.44EE72 pKa = 4.18LNRR75 pKa = 11.84IKK77 pKa = 10.5RR78 pKa = 11.84VSVNSYY84 pKa = 11.17ALDD87 pKa = 3.53PVYY90 pKa = 10.62RR91 pKa = 11.84EE92 pKa = 3.82SYY94 pKa = 10.13KK95 pKa = 10.54YY96 pKa = 10.52LRR98 pKa = 11.84QKK100 pKa = 10.7SKK102 pKa = 11.28EE103 pKa = 3.98PVKK106 pKa = 10.37TDD108 pKa = 3.19GQSNSACPRR117 pKa = 11.84RR118 pKa = 11.84HH119 pKa = 5.99CALFF123 pKa = 3.9

Molecular weight: 14.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4165

123

1861

595.0

67.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.994 ± 0.446

1.969 ± 0.306

6.771 ± 0.512

5.45 ± 0.231

4.706 ± 0.358

4.418 ± 0.321

2.449 ± 0.047

5.474 ± 0.351

6.074 ± 0.567

8.836 ± 0.762

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.425 ± 0.152

4.778 ± 0.429

4.97 ± 0.353

2.593 ± 0.282

5.69 ± 0.234

8.908 ± 0.269

5.09 ± 0.432

9.244 ± 0.802

0.672 ± 0.088

4.49 ± 0.421
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski