Amino acid dipepetide frequency for Microbacterium phage Arete

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.525AlaAla: 14.525 ± 1.519
0.119AlaCys: 0.119 ± 0.099
6.877AlaAsp: 6.877 ± 0.726
6.107AlaGlu: 6.107 ± 0.751
2.371AlaPhe: 2.371 ± 0.403
8.537AlaGly: 8.537 ± 1.109
1.423AlaHis: 1.423 ± 0.415
4.447AlaIle: 4.447 ± 0.569
3.439AlaLys: 3.439 ± 0.507
8.597AlaLeu: 8.597 ± 0.61
2.431AlaMet: 2.431 ± 0.55
4.624AlaAsn: 4.624 ± 0.658
5.099AlaPro: 5.099 ± 0.74
4.684AlaGln: 4.684 ± 0.608
6.225AlaArg: 6.225 ± 0.935
5.692AlaSer: 5.692 ± 0.866
6.522AlaThr: 6.522 ± 0.689
6.284AlaVal: 6.284 ± 0.589
1.719AlaTrp: 1.719 ± 0.281
2.134AlaTyr: 2.134 ± 0.34
0.0AlaXaa: 0.0 ± 0.0
Cys
0.474CysAla: 0.474 ± 0.207
0.0CysCys: 0.0 ± 0.0
0.119CysAsp: 0.119 ± 0.104
0.237CysGlu: 0.237 ± 0.138
0.119CysPhe: 0.119 ± 0.096
0.296CysGly: 0.296 ± 0.14
0.059CysHis: 0.059 ± 0.062
0.178CysIle: 0.178 ± 0.122
0.237CysLys: 0.237 ± 0.144
0.296CysLeu: 0.296 ± 0.155
0.0CysMet: 0.0 ± 0.0
0.237CysAsn: 0.237 ± 0.139
0.356CysPro: 0.356 ± 0.245
0.059CysGln: 0.059 ± 0.074
0.356CysArg: 0.356 ± 0.255
0.296CysSer: 0.296 ± 0.188
0.296CysThr: 0.296 ± 0.168
0.356CysVal: 0.356 ± 0.166
0.059CysTrp: 0.059 ± 0.075
0.237CysTyr: 0.237 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
7.648AspAla: 7.648 ± 0.854
0.356AspCys: 0.356 ± 0.202
3.972AspAsp: 3.972 ± 0.555
4.565AspGlu: 4.565 ± 0.527
2.371AspPhe: 2.371 ± 0.417
5.099AspGly: 5.099 ± 0.566
0.949AspHis: 0.949 ± 0.252
3.913AspIle: 3.913 ± 0.332
2.787AspLys: 2.787 ± 0.383
6.166AspLeu: 6.166 ± 0.863
1.186AspMet: 1.186 ± 0.263
2.49AspAsn: 2.49 ± 0.384
4.743AspPro: 4.743 ± 0.542
2.194AspGln: 2.194 ± 0.301
3.854AspArg: 3.854 ± 0.371
3.439AspSer: 3.439 ± 0.413
3.557AspThr: 3.557 ± 0.546
3.913AspVal: 3.913 ± 0.426
1.245AspTrp: 1.245 ± 0.401
1.719AspTyr: 1.719 ± 0.314
0.0AspXaa: 0.0 ± 0.0
Glu
5.217GluAla: 5.217 ± 0.788
0.178GluCys: 0.178 ± 0.109
3.202GluAsp: 3.202 ± 0.644
3.794GluGlu: 3.794 ± 0.571
2.846GluPhe: 2.846 ± 0.434
4.862GluGly: 4.862 ± 0.585
0.771GluHis: 0.771 ± 0.182
3.557GluIle: 3.557 ± 0.488
2.905GluLys: 2.905 ± 0.464
5.158GluLeu: 5.158 ± 0.521
1.66GluMet: 1.66 ± 0.322
2.609GluAsn: 2.609 ± 0.358
2.431GluPro: 2.431 ± 0.386
2.49GluGln: 2.49 ± 0.412
4.387GluArg: 4.387 ± 0.732
3.083GluSer: 3.083 ± 0.459
3.439GluThr: 3.439 ± 0.466
4.328GluVal: 4.328 ± 0.506
1.364GluTrp: 1.364 ± 0.32
2.075GluTyr: 2.075 ± 0.431
0.0GluXaa: 0.0 ± 0.0
Phe
2.787PheAla: 2.787 ± 0.383
0.296PheCys: 0.296 ± 0.147
3.202PheAsp: 3.202 ± 0.417
2.549PheGlu: 2.549 ± 0.387
1.304PhePhe: 1.304 ± 0.272
2.609PheGly: 2.609 ± 0.318
0.652PheHis: 0.652 ± 0.207
1.423PheIle: 1.423 ± 0.26
1.245PheLys: 1.245 ± 0.284
2.134PheLeu: 2.134 ± 0.265
0.889PheMet: 0.889 ± 0.261
1.304PheAsn: 1.304 ± 0.24
1.126PhePro: 1.126 ± 0.22
1.245PheGln: 1.245 ± 0.293
1.482PheArg: 1.482 ± 0.296
2.609PheSer: 2.609 ± 0.374
2.549PheThr: 2.549 ± 0.386
2.371PheVal: 2.371 ± 0.439
0.356PheTrp: 0.356 ± 0.142
0.949PheTyr: 0.949 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
7.174GlyAla: 7.174 ± 1.014
0.415GlyCys: 0.415 ± 0.219
4.624GlyAsp: 4.624 ± 0.65
4.624GlyGlu: 4.624 ± 0.468
2.253GlyPhe: 2.253 ± 0.346
7.174GlyGly: 7.174 ± 0.819
1.126GlyHis: 1.126 ± 0.323
3.913GlyIle: 3.913 ± 0.466
4.209GlyLys: 4.209 ± 0.813
6.225GlyLeu: 6.225 ± 0.755
1.956GlyMet: 1.956 ± 0.381
3.972GlyAsn: 3.972 ± 0.474
2.609GlyPro: 2.609 ± 0.314
3.202GlyGln: 3.202 ± 0.474
3.854GlyArg: 3.854 ± 0.538
5.158GlySer: 5.158 ± 0.588
6.284GlyThr: 6.284 ± 0.561
4.921GlyVal: 4.921 ± 0.532
2.253GlyTrp: 2.253 ± 0.311
2.905GlyTyr: 2.905 ± 0.43
0.0GlyXaa: 0.0 ± 0.0
His
1.067HisAla: 1.067 ± 0.311
0.119HisCys: 0.119 ± 0.087
0.949HisAsp: 0.949 ± 0.226
0.83HisGlu: 0.83 ± 0.266
0.534HisPhe: 0.534 ± 0.125
1.186HisGly: 1.186 ± 0.239
0.296HisHis: 0.296 ± 0.151
0.415HisIle: 0.415 ± 0.168
0.711HisLys: 0.711 ± 0.227
1.541HisLeu: 1.541 ± 0.52
0.296HisMet: 0.296 ± 0.11
0.237HisAsn: 0.237 ± 0.11
1.364HisPro: 1.364 ± 0.303
1.008HisGln: 1.008 ± 0.244
1.067HisArg: 1.067 ± 0.279
1.067HisSer: 1.067 ± 0.221
0.711HisThr: 0.711 ± 0.259
0.771HisVal: 0.771 ± 0.263
0.296HisTrp: 0.296 ± 0.136
0.771HisTyr: 0.771 ± 0.164
0.0HisXaa: 0.0 ± 0.0
Ile
4.565IleAla: 4.565 ± 0.582
0.059IleCys: 0.059 ± 0.064
3.261IleAsp: 3.261 ± 0.438
3.794IleGlu: 3.794 ± 0.559
1.126IlePhe: 1.126 ± 0.318
3.261IleGly: 3.261 ± 0.411
0.889IleHis: 0.889 ± 0.213
2.194IleIle: 2.194 ± 0.373
2.194IleLys: 2.194 ± 0.472
3.379IleLeu: 3.379 ± 0.503
1.482IleMet: 1.482 ± 0.362
2.194IleAsn: 2.194 ± 0.282
2.668IlePro: 2.668 ± 0.347
2.194IleGln: 2.194 ± 0.331
2.312IleArg: 2.312 ± 0.326
2.668IleSer: 2.668 ± 0.495
3.024IleThr: 3.024 ± 0.511
3.379IleVal: 3.379 ± 0.664
0.949IleTrp: 0.949 ± 0.207
1.186IleTyr: 1.186 ± 0.277
0.0IleXaa: 0.0 ± 0.0
Lys
4.447LysAla: 4.447 ± 0.46
0.178LysCys: 0.178 ± 0.121
2.431LysAsp: 2.431 ± 0.493
2.194LysGlu: 2.194 ± 0.51
0.83LysPhe: 0.83 ± 0.209
2.964LysGly: 2.964 ± 0.589
0.889LysHis: 0.889 ± 0.211
2.016LysIle: 2.016 ± 0.605
1.956LysLys: 1.956 ± 0.502
4.032LysLeu: 4.032 ± 0.55
1.541LysMet: 1.541 ± 0.274
1.956LysAsn: 1.956 ± 0.298
2.371LysPro: 2.371 ± 0.366
1.541LysGln: 1.541 ± 0.278
2.727LysArg: 2.727 ± 0.488
2.905LysSer: 2.905 ± 0.601
2.49LysThr: 2.49 ± 0.41
2.905LysVal: 2.905 ± 0.476
0.711LysTrp: 0.711 ± 0.173
1.364LysTyr: 1.364 ± 0.236
0.0LysXaa: 0.0 ± 0.0
Leu
6.877LeuAla: 6.877 ± 0.51
0.237LeuCys: 0.237 ± 0.144
5.573LeuAsp: 5.573 ± 0.43
5.692LeuGlu: 5.692 ± 0.537
3.202LeuPhe: 3.202 ± 0.485
5.632LeuGly: 5.632 ± 0.753
1.364LeuHis: 1.364 ± 0.313
4.209LeuIle: 4.209 ± 0.633
3.32LeuLys: 3.32 ± 0.394
4.921LeuLeu: 4.921 ± 0.483
1.779LeuMet: 1.779 ± 0.246
4.565LeuAsn: 4.565 ± 0.61
3.972LeuPro: 3.972 ± 0.475
4.091LeuGln: 4.091 ± 0.683
6.107LeuArg: 6.107 ± 0.656
4.506LeuSer: 4.506 ± 0.667
6.225LeuThr: 6.225 ± 0.554
4.862LeuVal: 4.862 ± 0.766
1.067LeuTrp: 1.067 ± 0.212
2.371LeuTyr: 2.371 ± 0.333
0.0LeuXaa: 0.0 ± 0.0
Met
3.439MetAla: 3.439 ± 0.58
0.237MetCys: 0.237 ± 0.128
1.008MetAsp: 1.008 ± 0.212
1.364MetGlu: 1.364 ± 0.269
1.364MetPhe: 1.364 ± 0.209
1.897MetGly: 1.897 ± 0.361
0.178MetHis: 0.178 ± 0.11
1.067MetIle: 1.067 ± 0.256
1.364MetLys: 1.364 ± 0.291
2.016MetLeu: 2.016 ± 0.317
0.949MetMet: 0.949 ± 0.347
0.593MetAsn: 0.593 ± 0.195
1.838MetPro: 1.838 ± 0.325
1.008MetGln: 1.008 ± 0.258
1.304MetArg: 1.304 ± 0.404
2.016MetSer: 2.016 ± 0.318
1.719MetThr: 1.719 ± 0.271
1.126MetVal: 1.126 ± 0.212
0.534MetTrp: 0.534 ± 0.172
0.534MetTyr: 0.534 ± 0.175
0.0MetXaa: 0.0 ± 0.0
Asn
4.387AsnAla: 4.387 ± 0.518
0.296AsnCys: 0.296 ± 0.185
2.609AsnAsp: 2.609 ± 0.392
1.779AsnGlu: 1.779 ± 0.398
1.245AsnPhe: 1.245 ± 0.285
4.15AsnGly: 4.15 ± 0.499
0.652AsnHis: 0.652 ± 0.215
1.956AsnIle: 1.956 ± 0.377
2.075AsnLys: 2.075 ± 0.346
4.565AsnLeu: 4.565 ± 0.631
0.949AsnMet: 0.949 ± 0.213
1.482AsnAsn: 1.482 ± 0.351
2.668AsnPro: 2.668 ± 0.314
2.075AsnGln: 2.075 ± 0.411
2.49AsnArg: 2.49 ± 0.42
3.024AsnSer: 3.024 ± 0.416
3.972AsnThr: 3.972 ± 0.672
3.439AsnVal: 3.439 ± 0.512
0.711AsnTrp: 0.711 ± 0.208
1.423AsnTyr: 1.423 ± 0.43
0.0AsnXaa: 0.0 ± 0.0
Pro
5.039ProAla: 5.039 ± 0.784
0.0ProCys: 0.0 ± 0.0
4.209ProAsp: 4.209 ± 0.54
3.024ProGlu: 3.024 ± 0.468
1.364ProPhe: 1.364 ± 0.244
3.913ProGly: 3.913 ± 0.39
0.711ProHis: 0.711 ± 0.242
2.253ProIle: 2.253 ± 0.417
2.371ProLys: 2.371 ± 0.416
3.913ProLeu: 3.913 ± 0.358
0.889ProMet: 0.889 ± 0.179
2.846ProAsn: 2.846 ± 0.463
2.668ProPro: 2.668 ± 0.677
1.897ProGln: 1.897 ± 0.413
2.312ProArg: 2.312 ± 0.42
3.083ProSer: 3.083 ± 0.404
3.557ProThr: 3.557 ± 0.432
3.794ProVal: 3.794 ± 0.443
0.889ProTrp: 0.889 ± 0.199
1.126ProTyr: 1.126 ± 0.222
0.0ProXaa: 0.0 ± 0.0
Gln
4.565GlnAla: 4.565 ± 0.722
0.296GlnCys: 0.296 ± 0.153
2.253GlnAsp: 2.253 ± 0.288
2.312GlnGlu: 2.312 ± 0.473
1.245GlnPhe: 1.245 ± 0.232
3.202GlnGly: 3.202 ± 0.473
1.304GlnHis: 1.304 ± 0.327
2.371GlnIle: 2.371 ± 0.236
1.66GlnLys: 1.66 ± 0.249
4.091GlnLeu: 4.091 ± 0.414
1.541GlnMet: 1.541 ± 0.446
2.312GlnAsn: 2.312 ± 0.505
1.897GlnPro: 1.897 ± 0.384
3.261GlnGln: 3.261 ± 0.518
2.549GlnArg: 2.549 ± 0.42
2.905GlnSer: 2.905 ± 0.453
3.202GlnThr: 3.202 ± 0.413
2.49GlnVal: 2.49 ± 0.527
0.652GlnTrp: 0.652 ± 0.179
1.245GlnTyr: 1.245 ± 0.197
0.0GlnXaa: 0.0 ± 0.0
Arg
5.632ArgAla: 5.632 ± 1.011
0.237ArgCys: 0.237 ± 0.17
4.209ArgAsp: 4.209 ± 0.446
4.15ArgGlu: 4.15 ± 0.578
2.253ArgPhe: 2.253 ± 0.329
4.684ArgGly: 4.684 ± 0.866
1.008ArgHis: 1.008 ± 0.193
2.312ArgIle: 2.312 ± 0.432
2.727ArgLys: 2.727 ± 0.382
4.921ArgLeu: 4.921 ± 0.571
1.719ArgMet: 1.719 ± 0.268
3.083ArgAsn: 3.083 ± 0.403
2.668ArgPro: 2.668 ± 0.463
2.787ArgGln: 2.787 ± 0.361
3.202ArgArg: 3.202 ± 0.472
3.32ArgSer: 3.32 ± 0.447
2.787ArgThr: 2.787 ± 0.45
3.735ArgVal: 3.735 ± 0.4
1.245ArgTrp: 1.245 ± 0.291
2.549ArgTyr: 2.549 ± 0.478
0.0ArgXaa: 0.0 ± 0.0
Ser
6.166SerAla: 6.166 ± 0.586
0.237SerCys: 0.237 ± 0.157
4.743SerAsp: 4.743 ± 0.505
3.676SerGlu: 3.676 ± 0.557
3.024SerPhe: 3.024 ± 0.465
4.802SerGly: 4.802 ± 0.993
0.771SerHis: 0.771 ± 0.214
3.024SerIle: 3.024 ± 0.377
2.312SerLys: 2.312 ± 0.473
4.506SerLeu: 4.506 ± 0.468
1.423SerMet: 1.423 ± 0.24
2.964SerAsn: 2.964 ± 0.495
2.846SerPro: 2.846 ± 0.449
3.142SerGln: 3.142 ± 0.423
3.379SerArg: 3.379 ± 0.377
4.624SerSer: 4.624 ± 0.63
3.379SerThr: 3.379 ± 0.538
5.099SerVal: 5.099 ± 0.671
1.897SerTrp: 1.897 ± 0.303
1.838SerTyr: 1.838 ± 0.299
0.0SerXaa: 0.0 ± 0.0
Thr
6.284ThrAla: 6.284 ± 0.692
0.237ThrCys: 0.237 ± 0.211
4.565ThrAsp: 4.565 ± 0.419
3.439ThrGlu: 3.439 ± 0.477
2.312ThrPhe: 2.312 ± 0.403
5.988ThrGly: 5.988 ± 0.777
1.008ThrHis: 1.008 ± 0.244
2.549ThrIle: 2.549 ± 0.488
2.609ThrLys: 2.609 ± 0.42
4.921ThrLeu: 4.921 ± 0.548
1.186ThrMet: 1.186 ± 0.235
2.549ThrAsn: 2.549 ± 0.326
3.854ThrPro: 3.854 ± 0.625
2.549ThrGln: 2.549 ± 0.385
3.617ThrArg: 3.617 ± 0.806
4.15ThrSer: 4.15 ± 0.604
5.039ThrThr: 5.039 ± 0.964
4.624ThrVal: 4.624 ± 0.479
1.008ThrTrp: 1.008 ± 0.22
2.371ThrTyr: 2.371 ± 0.495
0.0ThrXaa: 0.0 ± 0.0
Val
6.877ValAla: 6.877 ± 0.596
0.296ValCys: 0.296 ± 0.154
4.684ValAsp: 4.684 ± 0.418
3.735ValGlu: 3.735 ± 0.62
2.016ValPhe: 2.016 ± 0.318
4.921ValGly: 4.921 ± 0.533
0.593ValHis: 0.593 ± 0.179
2.253ValIle: 2.253 ± 0.344
2.49ValLys: 2.49 ± 0.488
5.336ValLeu: 5.336 ± 0.591
2.371ValMet: 2.371 ± 0.399
3.024ValAsn: 3.024 ± 0.516
3.498ValPro: 3.498 ± 0.461
3.498ValGln: 3.498 ± 0.557
4.447ValArg: 4.447 ± 0.453
5.336ValSer: 5.336 ± 0.485
3.498ValThr: 3.498 ± 0.501
4.506ValVal: 4.506 ± 0.635
0.889ValTrp: 0.889 ± 0.37
2.194ValTyr: 2.194 ± 0.324
0.0ValXaa: 0.0 ± 0.0
Trp
2.016TrpAla: 2.016 ± 0.289
0.059TrpCys: 0.059 ± 0.068
1.601TrpAsp: 1.601 ± 0.337
0.711TrpGlu: 0.711 ± 0.244
0.593TrpPhe: 0.593 ± 0.235
1.126TrpGly: 1.126 ± 0.262
0.059TrpHis: 0.059 ± 0.068
1.186TrpIle: 1.186 ± 0.215
0.771TrpLys: 0.771 ± 0.201
1.541TrpLeu: 1.541 ± 0.356
0.474TrpMet: 0.474 ± 0.16
1.423TrpAsn: 1.423 ± 0.271
0.296TrpPro: 0.296 ± 0.16
0.771TrpGln: 0.771 ± 0.178
1.423TrpArg: 1.423 ± 0.372
1.364TrpSer: 1.364 ± 0.392
1.008TrpThr: 1.008 ± 0.286
1.304TrpVal: 1.304 ± 0.445
0.178TrpTrp: 0.178 ± 0.09
0.83TrpTyr: 0.83 ± 0.22
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.668TyrAla: 2.668 ± 0.256
0.415TyrCys: 0.415 ± 0.169
2.075TyrAsp: 2.075 ± 0.36
1.779TyrGlu: 1.779 ± 0.266
0.771TyrPhe: 0.771 ± 0.239
2.549TyrGly: 2.549 ± 0.405
0.534TyrHis: 0.534 ± 0.235
1.541TyrIle: 1.541 ± 0.402
1.067TyrLys: 1.067 ± 0.312
2.312TyrLeu: 2.312 ± 0.255
0.771TyrMet: 0.771 ± 0.213
1.482TyrAsn: 1.482 ± 0.441
0.889TyrPro: 0.889 ± 0.231
1.66TyrGln: 1.66 ± 0.305
2.134TyrArg: 2.134 ± 0.309
2.609TyrSer: 2.609 ± 0.512
1.601TyrThr: 1.601 ± 0.413
2.253TyrVal: 2.253 ± 0.369
0.711TyrTrp: 0.711 ± 0.207
0.711TyrTyr: 0.711 ± 0.274
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (16868 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski