Arthrobacter phage Whytu

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8R2P5|A0A6G8R2P5_9CAUD Terminase OS=Arthrobacter phage Whytu OX=2713260 GN=1 PE=4 SV=1
MM1 pKa = 7.2TALFLKK7 pKa = 10.36NATVTVDD14 pKa = 4.02GEE16 pKa = 4.44DD17 pKa = 3.55ASEE20 pKa = 4.44DD21 pKa = 3.6ADD23 pKa = 3.91NVDD26 pKa = 4.61FTPTTSAATFTPISGNTQSDD46 pKa = 3.57SGATSWVCNMNIAQDD61 pKa = 3.83YY62 pKa = 7.18TAGSLFMTMFKK73 pKa = 11.0AEE75 pKa = 4.18GPVDD79 pKa = 3.98VVLKK83 pKa = 10.56PRR85 pKa = 11.84GTASGPTITATIVPVPAKK103 pKa = 10.28IGGGAGALTAAVSCQVVGKK122 pKa = 7.29PTIAPAVV129 pKa = 3.53

Molecular weight: 12.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8R2T1|A0A6G8R2T1_9CAUD Uncharacterized protein OS=Arthrobacter phage Whytu OX=2713260 GN=14 PE=4 SV=1
MM1 pKa = 7.1VVRR4 pKa = 11.84VQPSVEE10 pKa = 3.88ALDD13 pKa = 4.05AYY15 pKa = 9.86KK16 pKa = 10.74AVVLAMKK23 pKa = 10.63VIDD26 pKa = 3.74KK27 pKa = 9.81PIRR30 pKa = 11.84KK31 pKa = 9.35AINVDD36 pKa = 2.98TRR38 pKa = 11.84TTLSPVWKK46 pKa = 10.35KK47 pKa = 10.62LVTEE51 pKa = 4.2HH52 pKa = 7.28AGTLLDD58 pKa = 4.06QRR60 pKa = 11.84VLNTGTRR67 pKa = 11.84IAAGNPPAAIAGSSKK82 pKa = 10.24RR83 pKa = 11.84RR84 pKa = 11.84LSGGLVPAEE93 pKa = 4.08YY94 pKa = 10.98NRR96 pKa = 11.84MVEE99 pKa = 4.28FGVDD103 pKa = 3.45PKK105 pKa = 11.3DD106 pKa = 3.59KK107 pKa = 10.52NVPSEE112 pKa = 3.98YY113 pKa = 10.19SRR115 pKa = 11.84KK116 pKa = 7.35TKK118 pKa = 10.22SGKK121 pKa = 8.21TSTVRR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84TRR130 pKa = 11.84IGLPNARR137 pKa = 11.84EE138 pKa = 4.13KK139 pKa = 11.36GRR141 pKa = 11.84VVYY144 pKa = 9.03PAFADD149 pKa = 4.53FAPRR153 pKa = 11.84AISYY157 pKa = 7.61WVQSVVRR164 pKa = 11.84ITHH167 pKa = 5.01EE168 pKa = 4.01TLEE171 pKa = 4.37KK172 pKa = 10.64GSRR175 pKa = 3.44

Molecular weight: 19.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

4897

50

571

222.6

23.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.722 ± 0.825

0.49 ± 0.143

7.249 ± 0.385

5.105 ± 0.42

2.512 ± 0.167

9.251 ± 0.365

1.164 ± 0.192

4.493 ± 0.347

4.105 ± 0.578

7.515 ± 0.369

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.001 ± 0.126

3.267 ± 0.221

5.554 ± 0.545

2.471 ± 0.402

6.453 ± 0.73

5.269 ± 0.317

8.046 ± 0.658

8.372 ± 0.419

1.777 ± 0.289

2.185 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski