Salmonella virus STSR3
Average proteome isoelectric point is 7.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D5JRK0|A0A7D5JRK0_9CAUD Uncharacterized protein OS=Salmonella virus STSR3 OX=2743845 GN=STSR3_53 PE=4 SV=1
MM1 pKa = 7.1 TLEE4 pKa = 4.26 EE5 pKa = 5.03 ALGDD9 pKa = 3.75 CNYY12 pKa = 10.12 EE13 pKa = 4.1 QEE15 pKa = 4.59 CGAIFADD22 pKa = 3.74 PTTIVNDD29 pKa = 4.31 CGCDD33 pKa = 3.66 FDD35 pKa = 5.73 PNCPRR40 pKa = 11.84 CFPFNYY46 pKa = 9.2 VAAALAAAFYY56 pKa = 9.94 NLAMQSIAA64 pKa = 4.25
Molecular weight: 6.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.121
IPC2_protein 3.465
IPC_protein 3.274
Toseland 3.134
ProMoST 3.541
Dawson 3.325
Bjellqvist 3.503
Wikipedia 3.325
Rodwell 3.16
Grimsley 3.071
Solomon 3.21
Lehninger 3.16
Nozaki 3.503
DTASelect 3.605
Thurlkill 3.249
EMBOSS 3.338
Sillero 3.427
Patrickios 0.006
IPC_peptide 3.198
IPC2_peptide 3.376
IPC2.peptide.svr19 3.664
Protein with the highest isoelectric point:
>tr|A0A7D5FN15|A0A7D5FN15_9CAUD Lysozyme OS=Salmonella virus STSR3 OX=2743845 GN=STSR3_23 PE=4 SV=1
MM1 pKa = 7.79 AIVCSIKK8 pKa = 8.25 TTSQYY13 pKa = 10.75 PIRR16 pKa = 11.84 HH17 pKa = 5.97 WIIARR22 pKa = 11.84 EE23 pKa = 3.72 QLRR26 pKa = 11.84 RR27 pKa = 11.84 VNCVSEE33 pKa = 4.44 KK34 pKa = 8.44 ITRR37 pKa = 11.84 LILAARR43 pKa = 11.84 LLQALYY49 pKa = 11.18 GEE51 pKa = 4.93 IKK53 pKa = 9.97 MEE55 pKa = 3.98 LCFRR59 pKa = 11.84 RR60 pKa = 11.84 EE61 pKa = 3.8 SLRR64 pKa = 11.84 AFVIGRR70 pKa = 11.84 KK71 pKa = 9.81 GIGIEE76 pKa = 3.92 AGRR79 pKa = 11.84 VAAIAIVCSIKK90 pKa = 8.27 TTSQYY95 pKa = 10.75 PIRR98 pKa = 11.84 HH99 pKa = 5.97 WIIARR104 pKa = 11.84 EE105 pKa = 3.72 QLRR108 pKa = 11.84 RR109 pKa = 11.84 VNCVSEE115 pKa = 4.44 KK116 pKa = 8.44 ITRR119 pKa = 11.84 LILAARR125 pKa = 11.84 LLQALYY131 pKa = 11.18 GEE133 pKa = 4.93 IKK135 pKa = 9.97 MEE137 pKa = 3.97 LCFRR141 pKa = 11.84 RR142 pKa = 11.84 EE143 pKa = 3.73 YY144 pKa = 11.09 LRR146 pKa = 11.84 AFVIGRR152 pKa = 11.84 KK153 pKa = 9.81 GIGIEE158 pKa = 3.92 AGRR161 pKa = 11.84 VAAIAIVCSIKK172 pKa = 8.27 TTSQYY177 pKa = 10.75 PIRR180 pKa = 11.84 HH181 pKa = 5.97 WIIARR186 pKa = 11.84 EE187 pKa = 3.72 QLRR190 pKa = 11.84 RR191 pKa = 11.84 VNCVSEE197 pKa = 4.44 KK198 pKa = 8.44 ITRR201 pKa = 11.84 LILAARR207 pKa = 11.84 LLQALSGEE215 pKa = 4.47 INNVTGKK222 pKa = 10.55 DD223 pKa = 3.31 KK224 pKa = 10.86 TT225 pKa = 3.77
Molecular weight: 25.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.677
IPC_protein 10.555
Toseland 10.745
ProMoST 10.482
Dawson 10.833
Bjellqvist 10.57
Wikipedia 11.052
Rodwell 11.008
Grimsley 10.877
Solomon 10.965
Lehninger 10.935
Nozaki 10.76
DTASelect 10.555
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.774
Patrickios 10.716
IPC_peptide 10.979
IPC2_peptide 9.838
IPC2.peptide.svr19 8.549
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
85
0
85
14385
37
1055
169.2
19.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.682 ± 0.305
2.099 ± 0.153
4.491 ± 0.207
5.353 ± 0.314
3.907 ± 0.195
5.958 ± 0.208
2.315 ± 0.186
6.396 ± 0.239
6.931 ± 0.33
9.128 ± 0.4
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.857 ± 0.155
4.31 ± 0.142
3.066 ± 0.194
3.754 ± 0.242
7.918 ± 0.362
6.896 ± 0.257
5.874 ± 0.244
6.076 ± 0.205
1.62 ± 0.144
3.372 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here